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MBC in Press, published online ahead of print May 10, 2006
Mol. Biol. Cell 10.1091/mbc.E05-09-0906

A more recent version of this article appeared on July 1, 2006
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Submitted on October 3, 2005
Revised on April 11, 2006
Accepted on April 27, 2006

Aurora-B/AIM-1 Regulates the Dynamic Behavior of HP1{alpha} at the G2-M Transition

Yasuhiko Terada*{dagger}

*Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455; {dagger}Louis Pasteur Center for Medical Research, Department of Molecular Biology, Sakyo-ku, Kyoto 606, Japan

Monitoring Editor: Kerry Bloom

HP1 plays an important role in heterochromatin formation, and undergoes large-scale, progressive dissociation from heterochromatin in prophase cells. However, the mechanisms regulating the dynamic behavior of HP1 are poorly understood. In this study, the role of Aurora-B was investigated with respect to the dynamic behavior of HP1{alpha}. Mammalian Aurora-B, AIM-1, colocalizes with HP1{alpha} to the heterochromatin in G2. Depletion of Aurora-B/AIM-1 inhibited dissociation of HP1{alpha} from the chromosome arms at the G2-M transition. In addition, depletion of INCENP led to aberrant cellular localization of Aurora-B/AIM-1, but did not affect heterochromatin targeting of HP1{alpha}. It was proposed in the binary switch hypothesis, that phosphorylation of histone H3 at Ser-10 negatively regulates the binding of HP1{alpha} to the adjacent methylated Lys-9. However, Aurora-B/AIM-1-mediated phosphorylation of H3 induced dissociation of the HP1{alpha} chromodomain, but not of the intact protein in vitro, indicating that the center and/or C-terminal domain of HP1{alpha} interferes with the effect of H3 phosphorylation on HP1{alpha} dissociation. Interestingly, Lys-9 methyltransferase SUV39H1 is abnormally localized along the metaphase chromosome arms in Aurora-B/AIM-1 depleted cells. In conclusion, these results showed that Aurora-B/AIM-1 is necessary for regulated histone modifications involved in binding of HP1{alpha} by the N-terminus of histone H3 during mitosis.

Address correspondence to: Yasuhiko Terada (terad002{at}tc.umn.edu)




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Y. Terada and Y. Yasuda
Human Immunodeficiency Virus Type 1 Vpr Induces G2 Checkpoint Activation by Interacting with the Splicing Factor SAP145
Mol. Cell. Biol., November 1, 2006; 26(21): 8149 - 8158.
[Abstract] [Full Text] [PDF]


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