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MBC in Press, published online ahead of print February 15, 2006
Mol. Biol. Cell 10.1091/mbc.E05-10-0991

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Submitted on October 28, 2005
Accepted on February 3, 2006

Ran Is Required prior to Metaphase for Spindle Assembly and Chromosome Alignment and after Metaphase for Chromosome Segregation and Spindle Midbody Organization

Rosalind V. Silverman-Gavrila and Andrew Wilde

Department of Medical Genetics and Microbiology, University of Toronto, M5S 1A8 Toronto, Ontario, Canada

Monitoring Editor: Yixian Zheng

The Ran pathway has been shown to have a role in spindle assembly. However, the extent of the Ran pathway’s role in mitosis in vivo is unclear. We report that perturbation of the Ran pathway disrupted multiple steps of mitosis in syncytial Drosophila embryos and uncovered new mitotic processes which are regulated by Ran. During the onset of mitosis the Ran pathway is required for the production, organization and targeting of centrosomally nucleated microtubules to chromosomes. However, Ran’s role is not restricted to microtubule organization, as Ran is also required for the alignment of chromosomes at the metaphase plate. In addition, the Ran pathway is required for post-metaphase events including chromosome segregation and the assembly of the microtubule midbody. The Ran pathway mediates these mitotic events, in part, by facilitating the correct targeting of the kinase Aurora A and the kinesins KLP61F and KLP3A to spindles.

Address correspondence to: Andrew Wilde (andrew.wilde{at}utoronto.ca)




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