Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print April 26, 2006
Mol. Biol. Cell 10.1091/mbc.E05-11-1008

A more recent version of this article appeared on July 1, 2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E05-11-1008v1
17/7/2976    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frampton, J.
Right arrow Articles by Lehmann, A. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frampton, J.
Right arrow Articles by Lehmann, A. R.

Submitted on November 3, 2005
Revised on March 24, 2006
Accepted on April 13, 2006

Postreplication Repair and PCNA Modification in Schizosaccharomyces pombe

Jonathan Frampton,*{dagger} Anja Irmisch,* Catherine M. Green,* Andrea Neiss,{ddagger}{sect} Michelle Trickey,*|| Helle D. Ulrich,{ddagger}Kanji Furuya,* Felicity Z. Watts,* Antony M. Carr,* and Alan R. Lehmann*

*Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, United Kingdom; {ddagger}Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany; Clare Hall Laboratories, Cancer Research UK, South Mimms, Herts EN6 3LD, United Kingdom

Monitoring Editor: Orna Cohen-Fix

Ubiquitination of PCNA plays a crucial role in regulating replication past DNA damage in eukaryotes, but the detailed mechanisms appear to vary in different organisms. We have examined the modification of PCNA in Schizosaccharomyces pombe. We find that, in response to UV irradiation, PCNA is mono- and poly-ubiquitinated in a manner similar to that in S. cerevisiae. However in undamaged S. pombe cells, PCNA is ubiquitinated in S phase, whereas in S. cerevisiae it is sumoylated. Furthermore we find that, unlike in S. cerevisiae, mutants defective in ubiquitination of PCNA are also sensitive to ionising radiation, and PCNA is ubiquitinated following exposure of cells to ionising radiation, in a similar manner to the response to UV-irradiation. We show that PCNA modification and cell-cycle checkpoints represent two independent signals in response to DNA damage. Finally, we unexpectedly find that PCNA is ubiquitinated in response to DNA damage when cells are arrested in G2.

Present addresses: {dagger}AbCam, Cambridge Science Park, Milton Road, Cambridge, United Kingdom; ||Marie Curie Institute, The Chart, Oxted, Surrey RH8 0TL, United Kingdom; {sect}Heidelberg University Biochemistry Centre, 69120 Heidelberg, Germany.

Address correspondence to: Alan R. Lehmann (a.r.lehmann{at}sussex.ac.uk)




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Motegi, H.-J. Liaw, K.-Y. Lee, H. P. Roest, A. Maas, X. Wu, H. Moinova, S. D. Markowitz, H. Ding, J. H. J. Hoeijmakers, et al.
Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks
PNAS, August 26, 2008; 105(34): 12411 - 12416.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
X. H. Yang, B. Shiotani, M. Classon, and L. Zou
Chk1 and Claspin potentiate PCNA ubiquitination
Genes & Dev., May 1, 2008; 22(9): 1147 - 1152.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
Y. Tsuji, K. Watanabe, K. Araki, M. Shinohara, Y. Yamagata, T. Tsurimoto, F. Hanaoka, K.-i. Yamamura, M. Yamaizumi, and S. Tateishi
Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks.
Genes Cells, April 1, 2008; 13(4): 343 - 354.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
I. Chiolo, M. Saponaro, A. Baryshnikova, J.-H. Kim, Y.-S. Seo, and G. Liberi
The Human F-Box DNA Helicase FBH1 Faces Saccharomyces cerevisiae Srs2 and Postreplication Repair Pathway Roles
Mol. Cell. Biol., November 1, 2007; 27(21): 7439 - 7450.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kosoy, T. M. Calonge, E. A. Outwin, and M. J. O'Connell
Fission Yeast Rnf4 Homologs Are Required for DNA Repair
J. Biol. Chem., July 13, 2007; 282(28): 20388 - 20394.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. M. Lee, S. Nizza, T. Hayes, K. L. Bass, A. Irmisch, J. M. Murray, and M. J. O'Connell
Brc1-Mediated Rescue of Smc5/6 Deficiency: Requirement for Multiple Nucleases and a Novel Rad18 Function
Genetics, April 1, 2007; 175(4): 1585 - 1595.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. J. Chang, P. J. Lupardus, and K. A. Cimprich
Monoubiquitination of Proliferating Cell Nuclear Antigen Induced by Stalled Replication Requires Uncoupling of DNA Polymerase and Mini-chromosome Maintenance Helicase Activities
J. Biol. Chem., October 27, 2006; 281(43): 32081 - 32088.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.