Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print April 5, 2006
Mol. Biol. Cell 10.1091/mbc.E06-01-0005

A more recent version of this article appeared on June 1, 2006
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E06-01-0005v1
17/6/2770    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moissoglu, K.
Right arrow Articles by Schwartz, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moissoglu, K.
Right arrow Articles by Schwartz, M. A.

Submitted on January 3, 2006
Revised on March 14, 2006
Accepted on March 29, 2006

In Vivo Dynamics of Rac-Membrane Interactions

Konstadinos Moissoglu,* Boris M. Slepchenko,{dagger} Nahum Meller,* Alan F. Horwitz,{ddagger} and Martin A. Schwartz*{sect}||¶

*Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908; {dagger}Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030; Departments of {ddagger}Cell Biology, {sect}Microbiology, and ||Biomedical Engineering and Mellon Prostate Cancer Research Center, University of Virginia, Charlottesville, VA 22908

Monitoring Editor: Anne Ridley

The small GTPase Rac cycles between the membrane and the cytosol as it is activated by nucleotide exchange factors (GEFs) and inactivated by GTPase-activating proteins (GAPs). Solubility in the cytosol is conferred by binding of Rac to guanine-nucleotide dissociation inhibitors (GDIs). To analyze the in vivo dynamics of Rac, we developed a photobleaching method to measure the dissociation rate constant (Koff) of membrane-bound GFP-Rac. We find that Koff is 0.048 s-1 for wtRac and ~10-fold less (0.004 s-1) for G12VRac. Thus, the major route for dissociation is conversion of membrane-bound GTP-Rac to GDP-Rac, however, dissociation of GTP-Rac occurs at a detectable rate. Overexpression of the GEF Tiam1 unexpectedly decreased Koff for wtRac, most likely by converting membrane-bound GDP-Rac back to GTP-Rac. Both overexpression and shRNA-mediated suppression of RhoGDI strongly affected the amount of membrane-bound Rac but surprisingly had only slight effects on Koff. These results indicate that the RhoGDI controls Rac function mainly through effects on activation and/or membrane association.

Address correspondence to: Martin A. Schwartz (maschwartz{at}virginia.edu)




This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
S. Nola, M. Sebbagh, S. Marchetto, N. Osmani, C. Nourry, S. Audebert, C. Navarro, R. Rachel, M. Montcouquiol, N. Sans, et al.
Scrib regulates PAK activity during the cell migration process
Hum. Mol. Genet., November 15, 2008; 17(22): 3552 - 3565.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. P. Desai, K. E. Chapman, and C. M. Waters
Mechanical stretch decreases migration of alveolar epithelial cells through mechanisms involving Rac1 and Tiam1
Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L958 - L965.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Ugolev, Y. Berdichevsky, C. Weinbaum, and E. Pick
Dissociation of Rac1(GDP){middle dot}RhoGDI Complexes by the Cooperative Action of Anionic Liposomes Containing Phosphatidylinositol 3,4,5-Trisphosphate, Rac Guanine Nucleotide Exchange Factor, and GTP
J. Biol. Chem., August 8, 2008; 283(32): 22257 - 22271.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. C. Chae, J. H. Kim, K. L. Kim, H. W. Kim, H. Y. Lee, W. D. Heo, T. Meyer, P.-G. Suh, and S. H. Ryu
Phospholipase D Activity Regulates Integrin-mediated Cell Spreading and Migration by Inducing GTP-Rac Translocation to the Plasma Membrane
Mol. Biol. Cell, July 1, 2008; 19(7): 3111 - 3123.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
E. Syriani, A. Gomez-Cabrero, M. Bosch, A. Moya, E. Abad, A. Gual, X. Gasull, and M. Morales
Profilin induces lamellipodia by growth factor-independent mechanism
FASEB J, May 1, 2008; 22(5): 1581 - 1596.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-M. Kweon, Y. J. Cho, P. Minoo, J. Groffen, and N. Heisterkamp
Activity of the Bcr GTPase-activating Domain Is Regulated through Direct Protein/Protein Interaction with the Rho Guanine Nucleotide Dissociation Inhibitor
J. Biol. Chem., February 8, 2008; 283(6): 3023 - 3030.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
F. Chianale, S. Cutrupi, E. Rainero, G. Baldanzi, P. E. Porporato, S. Traini, N. Filigheddu, V. F. Gnocchi, M. M. Santoro, O. Parolini, et al.
Diacylglycerol Kinase-{alpha} Mediates Hepatocyte Growth Factor-induced Epithelial Cell Scatter by Regulating Rac Activation and Membrane Ruffling
Mol. Biol. Cell, December 1, 2007; 18(12): 4859 - 4871.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. M. Haugh
Membrane-Binding/Modification Model of Signaling Protein Activation and Analysis of Its Control by Cell Morphology
Biophys. J., June 1, 2007; 92(11): L93 - L95.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Lakhe-Reddy, S. Khan, M. Konieczkowski, G. Jarad, K. L. Wu, L. F. Reichardt, Y. Takai, L. A. Bruggeman, B. Wang, J. R. Sedor, et al.
beta8 Integrin Binds Rho GDP Dissociation Inhibitor-1 and Activates Rac1 to Inhibit Mesangial Cell Myofibroblast Differentiation
J. Biol. Chem., July 14, 2006; 281(28): 19688 - 19699.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.