Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print June 21, 2006
Mol. Biol. Cell 10.1091/mbc.E06-02-0145

A more recent version of this article appeared on September 1, 2006
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E06-02-0145v1
17/9/3756    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kondo-Okamoto, N.
Right arrow Articles by Okamoto, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kondo-Okamoto, N.
Right arrow Articles by Okamoto, K.

Submitted on February 17, 2006
Revised on June 7, 2006
Accepted on June 8, 2006

The Novel F-Box Protein Mfb1p Regulates Mitochondrial Connectivity and Exhibits Asymmetric Localization in Yeast

Noriko Kondo-Okamoto,* Kentaro Ohkuni,{dagger} Katsumi Kitagawa,{dagger} J. Michael McCaffery,{ddagger} Janet M. Shaw,* and Koji Okamoto*

*Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112; {dagger}Department of Molecular Pharmacology, St. Jude Children’s Research Hospital, Memphis, TN 38105; {ddagger}Integrated Imaging Center, Department of Biology, Johns Hopkins University, Baltimore, MD 21218

Monitoring Editor: Sandra Schmid

Although it is clear that mitochondrial morphogenesis is a complex process involving multiple proteins in eukaryotic cells, little is known about regulatory molecules that modulate mitochondrial network formation. Here we report the identification of a new yeast mitochondrial morphology gene called MFB1 (YDR219C). MFB1 encodes an F-box protein family member, many of which function in SCF (Skp1-Cdc53/Cullin-F-box protein) ubiquitin ligase complexes. F-box proteins also act in nonSCF complexes whose functions are not well understood. While cells lacking Mfb1p contain abnormally short mitochondrial tubules, Mfb1p is not essential for known pathways that determine mitochondrial morphology and dynamics. Mfb1p is peripherally associated with the mitochondrial surface. Coimmunoprecipitation assays reveal that Mfb1p interacts with Skp1p in an F-box-dependent manner. However, Mfb1p does not coimmunoprecipitate with Cdc53p. The F-box motif is not essential for Mfb1p-mediated mitochondrial network formation. These observations suggest that Mfb1p acts in a complex lacking Cdc53p required for mitochondrial morphogenesis. During budding, Mfb1p asymmetrically localizes to mother cell mitochondria. By contrast, Skp1p accumulates in the daughter cell cytoplasm. Mfb1p mother cell-specific asymmetry depends on the F-box motif, suggesting that Skp1p down-regulates Mfb1p mitochondrial association in buds. We propose that Mfb1p operates in a novel pathway regulating mitochondrial tubular connectivity.

Address correspondence to: Janet M. Shaw (shaw{at}biochem.utah.edu) or Koji Okamoto (koji{at}biochem.utah.edu)




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
S. Gandre-Babbe and A. M. van der Bliek
The Novel Tail-anchored Membrane Protein Mff Controls Mitochondrial and Peroxisomal Fission in Mammalian Cells
Mol. Biol. Cell, June 1, 2008; 19(6): 2402 - 2412.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. H.M. Ho, E. Giraud, R. Lister, D. Thirkettle-Watts, J. Low, R. Clifton, K. A. Howell, C. Carrie, T. Donald, and J. Whelan
Characterization of the Regulatory and Expression Context of an Alternative Oxidase Gene Provides Insights into Cyanide-Insensitive Respiration during Growth and Development
Plant Physiology, April 1, 2007; 143(4): 1519 - 1533.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Durr, M. Escobar-Henriques, S. Merz, S. Geimer, T. Langer, and B. Westermann
Nonredundant Roles of Mitochondria-associated F-Box Proteins Mfb1 and Mdm30 in Maintenance of Mitochondrial Morphology in Yeast
Mol. Biol. Cell, September 1, 2006; 17(9): 3745 - 3755.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.