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MBC in Press, published online ahead of print May 3, 2006
Mol. Biol. Cell 10.1091/mbc.E06-03-0170

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Submitted on March 6, 2006
Revised on April 17, 2006
Accepted on April 24, 2006

Cellular Analyses of the Mitotic Region in the C. elegans Adult Germ Line

Sarah L. Crittenden,* Kimberly A. Leonhard,{dagger} Dana T. Byrd,{dagger} and Judith Kimble*{dagger}{ddagger}{sect}

*Howard Hughes Medical Institute, {dagger}Department of Biochemistry, {ddagger}Laboratory of Molecular Biology, and {sect}Department of Genetics, University of Wisconsin-Madison, Madison, WI 53706

Monitoring Editor: Jean Schwarzbauer

The C. elegans germ line provides a model for understanding how signaling from a stem cell niche promotes continued mitotic divisions at the expense of differentiation. Here we report cellular analyses designed to identify germline stem cells within the germline mitotic region of adult hermaphrodites. Our results support several conclusions. First, all germ cells within the mitotic region are actively cycling, as visualized by BrdU labeling. No quiescent cells were found. Second, germ cells in the mitotic region lose BrdU label uniformly, either by movement of labeled cells into the meiotic region or by dilution, probably due to replication. No label-retaining cells were found in the mitotic region. Third, the distal tip cell niche extends processes that nearly encircle adjacent germ cells, a phenomenon that is likely to anchor the distal-most germ cells within the niche. Fourth, germline mitoses are not oriented reproducibly, even within the immediate confines of the niche. We propose that germ cells in the distal-most rows of the mitotic region serve as stem cells and more proximal germ cells embark on the path to differentiation. We also propose that C. elegans adult germline stem cells are maintained by proximity to the niche rather than by programmed asymmetric divisions.

Address correspondence to: Sarah L. Crittenden (slcritte{at}wisc.edu)




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