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MBC in Press, published online ahead of print August 16, 2006
Mol. Biol. Cell 10.1091/mbc.E06-03-0232

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Submitted on March 24, 2006
Revised on July 31, 2006
Accepted on August 4, 2006

The Neurospora crassa WC-1-dependent Blue Light Response Requires Acetylation of Histone H3 Lysine 14 by NGF-1

Benedetto Grimaldi,*{dagger} Pierluca Coiro,*{ddagger} Patrizia Filetici,{sect} Emanuela Berge,||¶ Joseph R. Dobosy,||# Michael Freitag,||@ Eric U. Selker,|| and Paola Ballario*{sect}

*Dipartimento di Genetica e Biologia Molecolare and {sect}Istituto di Biologia e Patologia Molecolari del Consiglio Nazionale Delle Ricerche, Università di Roma "La Sapienza," 00185 Roma, Italy; ||Institute of Molecular Biology, University of Oregon, Eugene, OR 97403

Monitoring Editor: William Tansey

Blue light-induced transcription in Neurospora crassa is regulated by the White Collar-1 (WC-1) photoreceptor. We report that residue K14 of histone H3 associated with the light-inducible albino-3 (al-3) promoter becomes transiently acetylated after photoinduction. This acetylation depends on WC-1. The relevance of this chromatin modification was directly evaluated in vivo by construction of a Neurospora strain with a mutated histone H3 gene (hH3K14Q). This strain phenocopies a wc-1 blind mutant and shows a strong reduction of light-induced transcriptional activation of both al-3 and vivid (vvd), another light inducible gene. We mutated ngf-1 (Neurospora GCN Five), which encodes a homologue of the yeast HAT Gcn5p, to generate a strain impaired in H3 K14 acetylation and found that it was defective in photoinduction. Altogether, our findings reveal a direct link between histone modification and light signaling in Neurospora and contribute to the developing understanding of the molecular mechanisms operating in light inducible gene activation.

Present addresses: {dagger}University of California, Irvine, 2115 Gillespie, Irvine, CA 92697; {ddagger}Charitè University of Berlin, Philippstrasse 12, D-10098 Berlin, Germany; #University of Wisconsin, 600 Highland Ave., CSC K6/528, Madison, WI 53792; MitoSciences Inc., 1850 Millrace Drive, Eugene, OR 97403; @Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331.

Address correspondence to: Paola Ballario (paola.ballario{at}uniroma1.it)




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