Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print May 10, 2006
Mol. Biol. Cell 10.1091/mbc.E06-03-0247

A more recent version of this article appeared on July 1, 2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E06-03-0247v1
17/7/3221    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lemm, I.
Right arrow Articles by Lührmann, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lemm, I.
Right arrow Articles by Lührmann, R.

Submitted on March 29, 2006
Revised on April 13, 2006
Accepted on April 27, 2006

Ongoing U snRNP Biogenesis Is Required for the Integrity of Cajal Bodies

Ira Lemm,* Cyrille Girard,{dagger} Andreas N. Kuhn,* Nicholas J. Watkins,{ddagger} Marc Schneider,* Rémy Bordonné,{dagger} and Reinhard Lührmann*

*Department of Cellular Biochemistry, Max-Planck-Institute for Biophysical Chemistry, D-37077 Göttingen, Germany; {dagger}Institut de Génétique Moléculaire, UMR5535 CNRS, F-34293 Montpellier, France; {ddagger}Institute for Cell and Molecular Biosciences, University of Newcastle, Newcastle upon Tyne, NE2 4HH, United Kingdom

Monitoring Editor: Susan Wente

Cajal bodies (CBs) have been implicated in the nuclear phase of the biogenesis of spliceosomal U small nuclear ribonucleoproteins (U snRNPs). Here, we have investigated the distribution of the CB marker protein coilin, U snRNPs, and proteins present in C/D box small nucleolar (sno)RNPs in cells depleted of hTGS1, SMN, or PHAX. Knock-down of any of these three proteins by RNAi interferes with U snRNP maturation before the reentry of U snRNA Sm cores into the nucleus. Strikingly, CBs are lost in the absence of hTGS1, SMN, or PHAX, and coilin is dispersed in the nucleoplasm into numerous small foci. This indicates that the integrity of canonical CBs is dependent on ongoing U snRNP biogenesis. Spliceosomal U snRNPs show no detectable concentration in nuclear foci and do not colocalize with coilin in cells lacking hTGS1, SMN, or PHAX. In contrast, C/D box snoRNP components concentrate into nuclear foci that partially colocalize with coilin after inhibition of U snRNP maturation. We demonstrate by siRNA-mediated depletion that coilin is required for the condensation of U snRNPs, but not C/D box snoRNP components, into nucleoplasmic foci, and also for merging these factors into canonical CBs. Altogether, our data suggest that CBs have a modular structure with distinct domains for spliceosomal U snRNPs and snoRNPs.

Address correspondence to: Reinhard Lührmann (reinhard.luehrmann{at}mpi-bpc.mpg.de)




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
D. Stanek, J. Pridalova-Hnilicova, I. Novotny, M. Huranova, M. Blazikova, X. Wen, A. K. Sapra, and K. M. Neugebauer
Spliceosomal Small Nuclear Ribonucleoprotein Particles Repeatedly Cycle through Cajal Bodies
Mol. Biol. Cell, June 1, 2008; 19(6): 2534 - 2543.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Girard, C. Verheggen, H. Neel, A. Cammas, S. Vagner, J. Soret, E. Bertrand, and R. Bordonne
Characterization of a Short Isoform of Human Tgs1 Hypermethylase Associating with Small Nucleolar Ribonucleoprotein Core Proteins and Produced by Limited Proteolytic Processing
J. Biol. Chem., January 25, 2008; 283(4): 2060 - 2069.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
S. Petri, M. Grimmler, S. Over, U. Fischer, and O. J. Gruss
Dephosphorylation of survival motor neurons (SMN) by PPM1G/PP2C{gamma} governs Cajal body localization and stability of the SMN complex
J. Cell Biol., November 5, 2007; 179(3): 451 - 465.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. J. Watkins, I. Lemm, and R. Luhrmann
Involvement of Nuclear Import and Export Factors in U8 Box C/D snoRNP Biogenesis
Mol. Cell. Biol., October 15, 2007; 27(20): 7018 - 7027.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
G. B. Gonsalvez, L. Tian, J. K. Ospina, F.-M. Boisvert, A. I. Lamond, and A. G. Matera
Two distinct arginine methyltransferases are required for biogenesis of Sm-class ribonucleoproteins
J. Cell Biol., August 27, 2007; 178(5): 733 - 740.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Z. Cui and P. J. DiMario
RNAi Knockdown of Nopp140 Induces Minute-like Phenotypes in Drosophila
Mol. Biol. Cell, June 1, 2007; 18(6): 2179 - 2191.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Hausmann, A. Ramirez, S. Schneider, B. Schwer, and S. Shuman
Biochemical and genetic analysis of RNA cap guanine-N2 methyltransferases from Giardia lamblia and Schizosaccharomyces pombe
Nucleic Acids Res., March 12, 2007; 35(5): 1411 - 1420.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.