Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print August 15, 2007
Mol. Biol. Cell 10.1091/mbc.E06-05-0416

A more recent version of this article appeared on November 1, 2007
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E06-05-0416v1
18/11/4222    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tholozan, F. M.D.
Right arrow Articles by Quinlan, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tholozan, F. M.D.
Right arrow Articles by Quinlan, R. A.

Submitted on May 15, 2006
Revised on July 24, 2007
Accepted on August 8, 2007

FGF-2 Release from the Lens Capsule by MMP-2 Maintains Lens Epithelial Cell Viability

Frederique M.D. Tholozan,* Christopher Gribbon,{dagger} Zheng Li,{ddagger} Martin W. Goldberg,* Alan R. Prescott,{sect} Norman McKie,{ddagger} and Roy A. Quinlan*

*School of Biological and Biomedical Sciences, Durham University, Durham DH1 3LE, United Kingdom; {ddagger}School of Clinical Medical Sciences (Gerontology), Henry Wellcome Laboratory for Biogerontology Research, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE, United Kingdom; {dagger}Protein Design Group, Department of Biochemistry, School of Life Sciences, University of Sussex, Falmer, BN1 9QG, United Kingdom; {sect}Centre for High Resolution Imaging and Processing, University of Dundee, Dundee DD1 5LE, United Kingdom

Monitoring Editor: M. Bishr Omary

The lens is an avascular tissue, separated from the aqueous and vitreous humors by its own extracellular matrix, the lens capsule. Here we demonstrate that the lens capsule is a source of essential survival factors for lens epithelial cells. Primary and immortalised lens epithelial cells survive in low levels of serum and are resistant to staurosporine-induced apoptosis when they remain in contact with the lens capsule. Physical contact with the capsule is required for maximal resistance to stress. The lens capsule is also a source of soluble factors including FGF-2 and perlecan, an ECM component that enhances FGF-2 activity. MMP-2 inhibition as well as MMP-2 pre-treatment of lens capsules greatly reduced the protective effect of the lens capsule, although this could be largely reversed by the addition of either conditioned medium or recombinant FGF-2. These data suggest that FGF-2 release from the lens capsule by MMP-2 is essential to lens epithelial cell viability and survival.

Address correspondence to: Roy A. Quinlan (r.a.quinlan{at}dur.ac.uk)




This article has been cited by other articles:


Home page
 IOVSHome page
M. Saint-Geniez, T. Kurihara, and P. A. D'Amore
Role of Cell and Matrix-Bound VEGF Isoforms in Lens Development
Invest. Ophthalmol. Vis. Sci., January 1, 2009; 50(1): 311 - 321.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2007 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.