Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print November 8, 2006
Mol. Biol. Cell 10.1091/mbc.E06-05-0461

A more recent version of this article appeared on January 1, 2007
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E06-05-0461v1
18/1/295    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Furuta, N.
Right arrow Articles by Tanaka, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Furuta, N.
Right arrow Articles by Tanaka, K.

Submitted on May 26, 2006
Revised on October 25, 2006
Accepted on October 26, 2006

Endocytic Recycling in Yeast Is Regulated by Putative Phospholipid Translocases and the Ypt31p/32p-Rcy1p Pathway

Nobumichi Furuta, Konomi Fujimura-Kamada, Koji Saito, Takaharu Yamamoto, and Kazuma Tanaka

Division of Molecular Interaction, Institute for Genetic Medicine, Hokkaido University Graduate School of Medicine, N15 W7, Kita-ku, Sapporo, 060-0815, Japan

Monitoring Editor: Sean Munro

Phospholipid translocases (PLTs) have been implicated in the generation of phospholipid asymmetry in membrane bilayers. In budding yeast, putative PLTs are encoded by the DRS2 gene family of type 4 P-type ATPases. The homologous proteins Cdc50p, Lem3p, and Crf1p are potential noncatalytic subunits of Drs2p, Dnf1p and Dnf2p, and Dnf3p, respectively; these putative heteromeric PLTs share an essential function for cell growth. We constructed temperature-sensitive mutants of CDC50 in the lem3{Delta} crf1{Delta} background (cdc50-ts mutants). Screening for multicopy suppressors of cdc50-ts identified YPT31/32, two genes that encode Rab family small GTPases that are involved in both the exocytic and endocytic recycling pathways. The cdc50-ts mutants did not exhibit major defects in the exocytic pathways, but did exhibit those in endocytic recycling; large membranous structures containing the v-SNARE Snc1p intracellularly accumulated in these mutants. Genetic results suggested that the YPT31/32 effector RCY1 and CDC50 function in the same signaling pathway, and simultaneous overexpression of CDC50, DRS2, and GFP-SNC1 restored growth as well as the plasma membrane localization of GFP-Snc1p in the rcy1{Delta} mutant. In addition, Rcy1p coimmunoprecipitated with Cdc50p-Drs2p. We propose that the Ypt31p/32p-Rcy1p pathway regulates putative phospholipid translocases to promote formation of vesicles destined for the trans-Golgi network from early endosomes.

Address correspondence to: Konomi Fujimura-Kamada (konomi{at}igm.hokudai.ac.jp) or Kazuma Tanaka (k-tanaka{at}igm.hokudai.ac.jp)




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
K. Liu, K. Surendhran, S. F. Nothwehr, and T. R. Graham
P4-ATPase Requirement for AP-1/Clathrin Function in Protein Transport from the trans-Golgi Network and Early Endosomes
Mol. Biol. Cell, August 1, 2008; 19(8): 3526 - 3535.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Ikeda, A. Kihara, A. Denpoh, and Y. Igarashi
The Rim101 Pathway Is Involved in Rsb1 Expression Induced by Altered Lipid Asymmetry
Mol. Biol. Cell, May 1, 2008; 19(5): 1922 - 1931.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. Nakano, T. Yamamoto, T. Kishimoto, T. Noji, and K. Tanaka
Protein Kinases Fpk1p and Fpk2p are Novel Regulators of Phospholipid Asymmetry
Mol. Biol. Cell, April 1, 2008; 19(4): 1783 - 1797.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.