Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print September 15, 2006
Mol. Biol. Cell 10.1091/mbc.E06-06-0486

A more recent version of this article appeared on November 1, 2006 Originally published as MBC in Press, 10.1091/mbc.E06-06-0486 on October 18, 2006 Originally published as MBC in Press, 10.1091/mbc.E06-06-0486 on September 13, 2006
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E06-06-0486v1
E06-06-0486v2
E06-06-0486v3
17/11/4812    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, H.
Right arrow Articles by Sodek, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, H.
Right arrow Articles by Sodek, J.

Submitted on June 2, 2006
Revised on July 31, 2006
Accepted on August 31, 2006

A Critical Role for MT1-MMP in Collagen Phagocytosis

Hyejin Lee,*{dagger} Christopher M. Overall,*{ddagger} Christopher A. McCulloch,* and Jaro Sodek*{dagger}

*Canadian Institutes of Health Research Group in Matrix Dynamics, Faculty of Dentistry, and the {dagger}Department of Biochemistry, Faculty of Medicine, University of Toronto, Toronto, Ontario M5S 3E2, Canada; {ddagger}University of British Columbia Centre for Blood Research, Vancouver, British Columbia V67 1Z3, Canada

Monitoring Editor: Asma Nusrat

Degradation of collagen is important for the physiological remodelling of connective tissues during growth and development, as well as in wound healing, inflammatory diseases and cancer cell invasion. In remodelling adult tissues, degradation of collagen occurs primarily through a phagocytic pathway. However, while various steps in the phagocytic pathway have been characterized, the enzyme required to initially fragment collagen fibrils for subsequent phagocytosis has not been identified. We have used laser confocal microscopy, TEM and biochemical assays to show that human fibroblasts initiate degradation of collagen through the collagenase activity of the membrane-bound metalloproteinase, MT1-MMP. Degradation of natural and reconstituted collagen substrates correlated with the expression of MT1-MMP, which was localized at sites of collagen cleavage at the surface of the cells and also within the cells, while collagen degradation was totally abrogated when MT1-MMP expression was blocked by siRNA treatment. In contrast to MT1-MMP, the gelatinolytic activity of MMP-2 was not required for collagen phagocytosis. These studies demonstrate a pivotal role of catalytically active MT1-MMP in preparing collagen fibrils for phagocytic degradation.

Address correspondence to: Jaro Sodek (jaro.sodek{at}utoronto.ca)




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
X.-Y. Li, I. Ota, I. Yana, F. Sabeh, and S. J. Weiss
Molecular Dissection of the Structural Machinery Underlying the Tissue-invasive Activity of Membrane Type-1 Matrix Metalloproteinase
Mol. Biol. Cell, August 1, 2008; 19(8): 3221 - 3233.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Nabavi, Y. Urukova, M. Cardelli, J. E. Aubin, and R. E. Harrison
Lysosome Dispersion in Osteoblasts Accommodates Enhanced Collagen Production during Differentiation
J. Biol. Chem., July 11, 2008; 283(28): 19678 - 19690.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-A. Cho, P. Osenkowski, H. Zhao, S. Kim, M. Toth, K. Cole, A. Aboukameel, A. Saliganan, L. Schuger, R. D. Bonfil, et al.
The Inactive 44-kDa Processed Form of Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) Enhances Proteolytic Activity via Regulation of Endocytosis of Active MT1-MMP
J. Biol. Chem., June 20, 2008; 283(25): 17391 - 17405.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Nacu, I. G. Luzina, K. Highsmith, V. Lockatell, K. Pochetuhen, Z. A. Cooper, M. P. Gillmeister, N. W. Todd, and S. P. Atamas
Macrophages Produce TGF-{beta}-Induced ({beta}-ig-h3) following Ingestion of Apoptotic Cells and Regulate MMP14 Levels and Collagen Turnover in Fibroblasts
J. Immunol., April 1, 2008; 180(7): 5036 - 5044.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. A. Wagenaar-Miller, L. H. Engelholm, J. Gavard, S. S. Yamada, J. S. Gutkind, N. Behrendt, T. H. Bugge, and K. Holmbeck
Complementary Roles of Intracellular and Pericellular Collagen Degradation Pathways In Vivo
Mol. Cell. Biol., September 15, 2007; 27(18): 6309 - 6322.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. H. Madsen, L. H. Engelholm, S. Ingvarsen, T. Hillig, R. A. Wagenaar-Miller, L. Kjoller, H. Gardsvoll, G. Hoyer-Hansen, K. Holmbeck, T. H. Bugge, et al.
Extracellular Collagenases and the Endocytic Receptor, Urokinase Plasminogen Activator Receptor-associated Protein/Endo180, Cooperate in Fibroblast-mediated Collagen Degradation
J. Biol. Chem., September 14, 2007; 282(37): 27037 - 27045.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.