Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print November 1, 2006
Mol. Biol. Cell 10.1091/mbc.E06-06-0554

A more recent version of this article appeared on January 1, 2007
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E06-06-0554v1
18/1/106    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nazarko, T. Y.
Right arrow Articles by Sibirny, A. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nazarko, T. Y.
Right arrow Articles by Sibirny, A. A.

Submitted on June 26, 2006
Revised on October 13, 2006
Accepted on October 19, 2006

The Requirement of Sterol Glucoside for Pexophagy in Yeasts Is Dependent on the Species and Nature of Peroxisome Inducers

Taras Y. Nazarko,*{dagger} Andriy S. Polupanov,{dagger} Ravi R. Manjithaya,* Suresh Subramani,* and Andrei A. Sibirny{dagger}{ddagger}

*Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093-0322; {dagger}Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005, Lviv, Ukraine; {ddagger}Department of Metabolic Engineering, Rzeszow University, Cwiklinskiej 2, Rzeszow 3-601, Poland

Monitoring Editor: Benjamin Glick

Sterol glucosyltransferase, Ugt51/Atg26, is essential for both micropexophagy and macropexophagy of methanol-induced peroxisomes in Pichia pastoris. However, the role of this protein in pexophagy in other yeasts remained unclear. We show that oleate- and amine-induced peroxisomes in Yarrowia lipolytica are degraded by Atg26-independent macropexophagy. Surprisingly, Atg26 was also not essential for macropexophagy of oleate- and amine-induced peroxisomes in P. pastoris suggesting that the function of sterol glucoside (SG) in pexophagy is both species- and peroxisome inducer-specific. However, the rates of degradation of oleate- and amine-induced peroxisomes in P. pastoris were reduced in the absence of SG indicating that P. pastoris specifically utilizes sterol conversion by Atg26 to enhance selective degradation of peroxisomes. However, methanol-induced peroxisomes apparently have lost the redundant ability to be degraded without SG. We also show that the P. pastoris Vac8 armadillo repeat protein is not essential for macropexophagy of methanol-, oleate- or amine-induced peroxisomes, which makes PpVac8 the first known protein required for the micropexophagy, but not for the macropexophagy, machinery. The uniqueness of Atg26 and Vac8 functions under different pexophagy conditions demonstrates that not only pexophagy inducers, such as glucose or ethanol, but also the inducers of peroxisomes, such as methanol, oleate or primary amines, determine the requirements for subsequent pexophagy in yeasts.

Address correspondence to: Suresh Subramani (ssubramani{at}ucsd.edu)






Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.