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MBC in Press, published online ahead of print October 4, 2006
Mol. Biol. Cell 10.1091/mbc.E06-08-0665

A more recent version of this article appeared on December 1, 2006
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Submitted on August 2, 2006
Revised on September 13, 2006
Accepted on September 27, 2006

Transition of Galactosyltransferase 1 from Trans-Golgi Cisterna to the Trans-Golgi Network Is Signal Mediated

Beat E. Schaub, Bea Berger, Eric G. Berger, and Jack Rohrer

Institute of Physiology, University of Zurich, CH-8057 Zurich, Switzerland

Monitoring Editor: Vivek Malhotra

The Golgi apparatus (GA) is the organelle where complex glycan formation takes place. In addition, it is a major sorting site for proteins destined for various subcellular compartments or for secretion. Here we investigate {beta}1,4-galactosyltransferase 1 (galT) and {alpha}2,6-sialyltransferase 1 (siaT), two trans-Golgi glycosyltransferases, with respect to their different pathways in monensin treated cells. On addition of monensin galT dissociates from siaT and the GA and accumulates in swollen vesicles derived from the trans-Golgi network (TGN), as shown by colocalization with TGN46, a specific TGN marker. We analyzed various chimeric constructs of galT and siaT by confocal fluorescence microscopy and time lapse video-microscopy as well as Optiprep density gradient fractionation. We show that the first 13 amino acids of the cytoplasmic tail of galT are necessary and sufficient for its localization to swollen vesicles induced by monensin. We also show that the monensin sensitivity resulting from these 13 amino acids can be conferred to siaT, which leads to the rapid accumulation of the galT-siaT chimera in swollen vesicles upon monensin treatment. Based on these data we suggest that cycling between the trans-Golgi cisterna and the trans-Golgi network of galT is signal mediated.

Address correspondence to: Jack Rohrer (rohrer.jack{at}access.unizh.ch)




This article has been cited by other articles:


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Distinct Functions for Arf Guanine Nucleotide Exchange Factors at the Golgi Complex: GBF1 and BIGs Are Required for Assembly and Maintenance of the Golgi Stack and trans-Golgi Network, Respectively
Mol. Biol. Cell, February 1, 2008; 19(2): 523 - 535.
[Abstract] [Full Text] [PDF]


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