Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print December 6, 2006
Mol. Biol. Cell 10.1091/mbc.E06-09-0788

A more recent version of this article appeared on February 1, 2007
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Materials
Right arrow All Versions of this Article:
E06-09-0788v1
18/2/658    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Toshima, J.
Right arrow Articles by Drubin, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Toshima, J.
Right arrow Articles by Drubin, D. G.

Submitted on September 6, 2006
Revised on November 15, 2006
Accepted on November 27, 2006

Negative Regulation of Yeast Eps15-like Arp2/3 Complex Activator, Pan1p, by the Hip1R-related Protein, Sla2p, during Endocytosis

Jiro Toshima,*{dagger} Junko Y. Toshima,*{dagger} Mara C. Duncan,* Jamie T.V. Cope,* Yidi Sun,* Adam C. Martin,* Scott Anderson,{ddagger} John R. Yates III,{ddagger} Kensaku Mizuno,{dagger} and David G. Drubin*

*Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202; {ddagger}Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037; {dagger}Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980-8578, Japan

Monitoring Editor: Charles Boone

Control of actin assembly nucleated by the Arp2/3 complex plays a crucial role during budding yeast endocytosis. The yeast Eps15-related Arp2/3 complex activator, Pan1p, is essential for endocytic internalization and proper actin organization. Pan1p activity is negatively regulated by Prk1 kinase phosphorylation after endocytic internalization. Phosphorylated Pan1p is probably then dephosphorylated in the cytosol. Pan1p is recruited to endocytic sites ~25 s before initiation of actin polymerization, suggesting that its Arp2/3 complex activation activity is kept inactive during early stages of endocytosis by a yet-to-be-identified mechanism. However, how Pan1p is maintained in an inactive state is not clear. Using Tandem Affinity Purification (TAP)-tagged Pan1p, we identified End3p as a stoichiometric component of the Pan1p complex, and Sla2p, a yeast Hip1R-related protein, as a novel binding partner of Pan1p. Interestingly, Sla2p specifically inhibited Pan1p Arp2/3 complex activation activity in vitro. The coiled-coil region of Sla2p was important for Pan1p inhibition, and a pan1 partial loss-of-function mutant suppressed the temperature sensitivity, endocytic and actin phenotypes observed in sla2{Delta}CC mutant cells that lack the coiled-coil region. Overall, our results establish that Sla2p’s regulation of Pan1p plays an important role in controlling Pan1p-stimulated actin polymerization during endocytosis.

Address correspondence to: David G. Drubin (drubin{at}socrates.berkeley.edu)




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
L. Maldonado-Baez, M. R. Dores, E. M. Perkins, T. G. Drivas, L. Hicke, and B. Wendland
Interaction between Epsin/Yap180 Adaptors and the Scaffolds Ede1/Pan1 Is Required for Endocytosis
Mol. Biol. Cell, July 1, 2008; 19(7): 2936 - 2948.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Jin and M. Cai
A Novel Function of Arp2p in Mediating Prk1p-specific Regulation of Actin and Endocytosis in Yeast
Mol. Biol. Cell, January 1, 2008; 19(1): 297 - 307.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
G. Zeng, B. Huang, S. P. Neo, J. Wang, and M. Cai
Scd5p Mediates Phosphoregulation of Actin and Endocytosis by the Type 1 Phosphatase Glc7p in Yeast
Mol. Biol. Cell, December 1, 2007; 18(12): 4885 - 4898.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. L. Repass, R. J. Brady, and T. J. O'Halloran
Dictyostelium Hip1r contributes to spore shape and requires epsin for phosphorylation and localization
J. Cell Sci., November 15, 2007; 120(22): 3977 - 3988.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.