Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print December 20, 2006
Mol. Biol. Cell 10.1091/mbc.E06-09-0798

A more recent version of this article appeared on March 1, 2007
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E06-09-0798v1
18/3/743    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johnson, M. B.
Right arrow Articles by Enns, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johnson, M. B.
Right arrow Articles by Enns, C. A.

Submitted on September 8, 2006
Revised on November 17, 2006
Accepted on December 3, 2006

Transferrin Receptor 2: Evidence for Ligand-induced Stabilization and Redirection to a Recycling Pathway

Martha B. Johnson,* Juxing Chen,{dagger} Nicholas Murchison,{dagger} Frank A. Green,{dagger} and Caroline A. Enns{dagger}

Departments of *Biochemistry and Molecular Biology and {dagger}Cell and Developmental Biology, Oregon Health & Science University, Portland, OR 97239

Monitoring Editor: Jean Gruenberg

Transferrin receptor 2 (TfR2) is a homolog of transferrin receptor 1 (TfR1), the protein that delivers iron to cells through receptor-mediated endocytosis of diferric transferrin (Fe2Tf). TfR2 also binds Fe2Tf, but appears to function primarily in the regulation of systemic iron homeostasis. In contrast to TfR1, the trafficking of TfR2 within the cell has not been extensively characterized. Previously, we showed that Fe2Tf increases TfR2 stability, suggesting that trafficking of TfR2 may be regulated by interaction with its ligand. In the present study, therefore, we sought to identify the mode of TfR2 degradation, characterize TfR2 trafficking, and determine how Fe2Tf stabilizes TfR2. Stabilization of TfR2 by bafilomycin implies that TfR2 traffics to the lysosome for degradation. Confocal microscopy reveals that treatment of cells with Fe2Tf increases the fraction of TfR2 localizing to recycling endosomes and decreases the fraction of TfR2 localizing to late endosomes. Mutational analysis of TfR2 shows that the mutation G679A, which blocks TfR2 binding to Fe2Tf, increases the rate of receptor turnover and prevents stabilization by Fe2Tf, indicating a direct role of Fe2Tf in TfR2 stabilization. The mutation Y23A in the cytoplasmic domain of TfR2 inhibits its internalization and degradation, implicating YQRV as an endocytic motif.

Address correspondence to: Caroline A. Enns (ennsca{at}ohsu.edu)




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
D. G. Robinson, L. Jiang, and K. Schumacher
The Endosomal System of Plants: Charting New and Familiar Territories
Plant Physiology, August 1, 2008; 147(4): 1482 - 1492.
[Full Text] [PDF]

Home page
 BloodHome page
N. C. Andrews
Forging a field: the golden age of iron biology
Blood, July 15, 2008; 112(2): 219 - 230.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. F. Wallace, L. Summerville, E. M. Crampton, and V. N. Subramaniam
Defective trafficking and localization of mutated transferrin receptor 2: implications for type 3 hereditary hemochromatosis
Am J Physiol Cell Physiol, February 1, 2008; 294(2): C383 - C390.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Chen, M. Chloupkova, J. Gao, T. L. Chapman-Arvedson, and C. A. Enns
HFE Modulates Transferrin Receptor 2 Levels in Hepatoma Cells via Interactions That Differ from Transferrin Receptor 1-HFE Interactions
J. Biol. Chem., December 21, 2007; 282(51): 36862 - 36870.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.