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MBC in Press, published online ahead of print February 14, 2007
Mol. Biol. Cell 10.1091/mbc.E06-10-0890

A more recent version of this article appeared on May 1, 2007 Originally published as MBC in Press, 10.1091/mbc.E06-10-0890 on February 21, 2007
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Submitted on October 4, 2006
Revised on February 6, 2007
Accepted on February 7, 2007

Shugoshin2 Regulates Localization of the Chromosomal Passenger Proteins in Fission Yeast Mitosis

Vincent Vanoosthuyse, Sergey Prykhozhij, and Kevin G. Hardwick

Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom

Monitoring Editor: Yixian Zheng

Fission yeast has two members of the Shugoshin family, Sgo1 and Sgo2. Although Sgo1 has clearly been established as a protector of centromere cohesion in meiosis I ((Kitajima et al., 2004; Rabitsch et al., 2004; Vaur et al., 2005)), the roles of Sgo2 remain elusive. Here we show that Sgo2 is required to ensure proper chromosome biorientation upon recovery from a prolonged spindle checkpoint arrest. Consistent with this, Sgo2 is essential for maintaining the Passenger proteins on centromeres upon checkpoint activation. Interestingly, lack of Sgo2 has a more penetrant effect on the localization of Survivin than on the two other Passenger proteins INCENP and Aurora B, and the Survivin-INCENP complex but not the INCENP-Aurora B complex is destabilised in the absence of Sgo2. Finally we show that the conserved C-terminus of Sgo2 is crucial to maintain Sgo2 and Passenger proteins localization on centromeres upon prolonged checkpoint activation. Taken together, our results demonstrate that Sgo2 is important for chromosome biorientation and that it controls docking of the Passenger proteins on chromosomes in early mitotic cells.

Address correspondence to: Vincent Vanoosthuyse (fvvanoost{at}staffmail.ed.ac.uk)




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