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MBC in Press, published online ahead of print February 21, 2007
Mol. Biol. Cell 10.1091/mbc.E06-10-0963

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Submitted on October 27, 2006
Revised on January 16, 2007
Accepted on February 9, 2007

Chromosome Malorientations following Meiosis-II Arrest Cause Nondisjunction

Marie A. Janicke,* Loren Lasko,* Rudolf Oldenbourg,{dagger} and James R. LaFountain Jr.*

*Department of Biological Sciences, State University of New York at Buffalo, Amherst, NY 14260; {dagger}Marine Biological Laboratory, Woods Hole, MA 02543

Monitoring Editor: Kerry Bloom

This study investigated the basis of meiosis-II nondisjunction. Cold arrest induced a fraction of meiosis-II crane-fly spermatocytes to form (n + 1) and (n-1) daughters during recovery. Live-cell LC-PolScope imaging showed nondisjunction was caused by chromosome malorientation. Whereas amphitely (sister kinetochore fibers to opposite poles) is normal, cold recovery induced anaphase syntely (sister fibers to the same pole) and merotely (fibers to both poles from one kinetochore). Maloriented chromosomes had stable metaphase positions near the equator or between the equator and a pole. Syntelics were at the spindle periphery at metaphase; their sisters disconnected at anaphase and moved all the way to a centrosome, as their strongly birefringent kinetochore fibers shortened. The kinetochore fibers of merotelics shortened little if any during anaphase, making anaphase lag common. If one fiber of a merotelic was more birefringent than the other, the less birefringent fiber lengthened with anaphase spindle elongation, often permitting inclusion of merotelics in a daughter nucleus. Meroamphitely (near amphitely but with some merotely) caused sisters to move in opposite directions. In contrast, syntely and merosyntely (near syntely but with some merotely) resulted in nondisjunction. Anaphase malorientations were more frequent following longer arrests, with particularly long arrests required to induce syntely and merosyntely.

Address correspondence to: Marie A. Janicke (mjanicke{at}buffalo.edu)






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