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MBC in Press, published online ahead of print April 4, 2007
Mol. Biol. Cell 10.1091/mbc.E06-11-1039

A more recent version of this article appeared on June 1, 2007
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Submitted on November 27, 2006
Revised on March 16, 2007
Accepted on March 26, 2007

The Peroxiredoxin Tpx1 Is Essential as a H2O2-Scavenger during Aerobic Growth in Fission Yeast

Mónica Jara,* Ana P. Vivancos,* Isabel A. Calvo, Alberto Moldón, Miriam Sansó, and Elena Hidalgo

Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, E-08003 Barcelona, Spain

Monitoring Editor: Thomas Fox

Peroxiredoxins are known to interact with H2O2 and participate in oxidant scavenging, redox signal transduction and heat shock responses. The 2-cysteine peroxiredoxin Tpx1 of Schizosaccharomyces pombe has been characterized as the H2O2 sensor that transduces the redox signal to the transcription factor Pap1. Here, we show that Tpx1 is essential for aerobic, but not anaerobic, growth. We demonstrate that Tpx1 has an exquisite sensitivity for its substrate, which explains its participation in maintaining low steady-state levels of H2O2. We also show in vitro and in vivo that inactivation of Tpx1 by oxidation of its catalytic cysteine to a sulfinic acid is always preceded by a sulfinic acid form in a covalently linked dimer, which may be important for understanding the kinetics of Tpx1 inactivation. Furthermore, we provide evidence that a strain expressing Tpx1.C169S, lacking the resolving cysteine, can sustain aerobic growth, and we show that small reductants can modulate the activity of the mutant protein in vitro, probably by supplying a thiol group to substitute for cysteine 169.

*These authors contributed equally to this work.

Address correspondence to: Elena Hidalgo (elena.hidalgo{at}upf.edu)




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M. Jara, A. P. Vivancos, and E. Hidalgo
C-terminal truncation of the peroxiredoxin Tpx1 decreases its sensitivity for hydrogen peroxide without compromising its role in signal transduction.
Genes Cells, February 1, 2008; 13(2): 171 - 179.
[Abstract] [Full Text] [PDF]


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