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MBC in Press, published online ahead of print March 7, 2007
Mol. Biol. Cell 10.1091/mbc.E07-01-0019

A more recent version of this article appeared on May 1, 2007
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Submitted on January 12, 2007
Revised on February 16, 2007
Accepted on February 27, 2007

Interphase-specific Phosphorylation-mediated Regulation of Tubulin Dimer Partitioning in Human Cells

Per Holmfeldt, Sonja Stenmark, and Martin Gullberg

Department of Molecular Biology, Umeå University, SE-901 87 Umeå, Sweden

Monitoring Editor: Yixian Zheng

The microtubule cytoskeleton is differentially regulated by a diverse array of proteins during interphase and mitosis. Op18/stathmin (Op18) and MAP4 have been ascribed opposite general microtubule-directed activities, namely microtubule destabilization and stabilization, respectively, both of which can be inhibited by phosphorylation. Here, using three human cell models, we depleted cells of Op18 and/or MAP4 by expression of interfering hairpin RNAs and analyzed the resulting phenotypes. We found that the endogenous levels of Op18 and MAP4 have opposite and counteractive activities that largely govern the partitioning of tubulin dimers in the microtubule array at interphase. Op18 and MAP4 were also found to be the downstream targets of CaM kinase IV and PAR-1/MARK2 kinase, respectively, that control the demonstrated counteractive phosphorylation-mediated regulation of tubulin dimer partitioning. Furthermore, to address mechanisms regulating microtubule polymerization in response to cell signals, we developed a system for inducible gene product replacement. This approach revealed that site-specific phosphorylation of Op18 is both necessary and sufficient for polymerization of microtubules in response to the multifaceted signaling event of stimulation of the T-cell antigen receptor complex, which activates several signal transduction pathways.

Address correspondence to: Per Holmfeldt (per.holmfeldt{at}molbiol.umu.se)




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M. E. Sellin, P. Holmfeldt, S. Stenmark, and M. Gullberg
Global Regulation of the Interphase Microtubule System by Abundantly Expressed Op18/Stathmin
Mol. Biol. Cell, July 1, 2008; 19(7): 2897 - 2906.
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