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MBC in Press, published online ahead of print June 27, 2007
Mol. Biol. Cell 10.1091/mbc.E07-01-0070

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Submitted on January 26, 2007
Revised on June 18, 2007
Accepted on June 19, 2007

Small Interfering RNA-mediated Silencing Induces Target-dependent Assembly of GW/P Bodies

Shangli Lian,* Marvin J. Fritzler,{dagger} Joseph Katz,{ddagger} Takashi Hamazaki,{sect} Naohiro Terada,{sect} Minoru Satoh,{sect}|| and Edward K.L. Chan*

*Departments of Oral Biology, {ddagger}Oral Medicine, and {sect}Pathology, Immunology, and Laboratory Medicine, and ||Division of Rheumatology and Clinical Immunology, Department of Medicine, University of Florida, Gainesville, FL 32610; {dagger}Department of Biochemistry and Molecular Biology, University of Calgary, Alberta, Canada T2N 1N4

Monitoring Editor: A. Gregory Matera

Gene silencing using small interfering RNA is a valuable laboratory tool and a promising approach to therapeutics for a variety of human diseases. Recently, RNA interference has been linked to cytoplasmic GW bodies. However, the correlation between RNA interference and the formation of GW bodies, also known as mammalian processing bodies, remains unclear. In this report we show that transfection of functional small interfering RNA induced larger and greater numbers of GW bodies. This siRNA-induced increase of GW bodies depends on the endogenous expression of the target mRNA. Knockdown of GW182 or Ago2 demonstrated that the siRNA-induced increase of GW bodies required these two proteins and correlated with RNA interference. Furthermore, knockdown of rck/p54 or LSm1 did not prevent the reassembly of GWB that were induced by and correlated with siRNA-mediated RNA silencing. We propose that RNA interference is a key regulatory mechanism for the assembly of GW bodies and in some cases GWB may serve as markers for RNA interference in mammalian cells.

Address correspondence to: Edward K.L. Chan (echan{at}ufl.edu)




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