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MBC in Press, published online ahead of print August 15, 2007
Mol. Biol. Cell 10.1091/mbc.E07-01-0080

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Submitted on January 29, 2007
Revised on July 20, 2007
Accepted on August 2, 2007

Rab6 Regulates Both ZW10/RINT-1 and COG Complex Dependent Golgi Trafficking and Homeostasis

Yi Sun, Anna Shestakova, Lauren Hunt, Siddharth Sehgal, Vladimir Lupashin, and Brian Storrie

Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR 72205

Monitoring Editor: Francis Barr

We used multiple approaches to investigate the role of Rab6 relative to ZW10, a mitotic checkpoint protein implicated in Golgi/ER trafficking/transport, and Conserved Oligomeric Golgi (COG) complex, a putative tether in retrograde, intraGolgi trafficking. ZW10 depletion resulted in a central, disconnected cluster of Golgi elements and inhibition of ERGIC53 and Golgi enzyme recycling to ER. siRNA against RINT-1, a protein linker between ZW10 and the ER SNARE, syntaxin 18, produced similar Golgi disruption. COG3 depletion fragmented the Golgi and produced vesicles; vesicle formation was unaffected by codepletion of ZW10 along with COG, suggesting ZW10 and COG act separately. Rab6 depletion did not significantly affect Golgi ribbon organization. Epistatic depletion of Rab6 inhibited the Golgi disruptive effects of ZW10/RINT-1 siRNA or COG inactivation by siRNA or antibodies. Dominant negative expression of GDP-Rab6 suppressed ZW10 knockdown induced-Golgi disruption. No cross-talk was observed between Rab6 and endosomal Rab5 and Rab6 depletion failed to suppress p115 (anterograde tether) knockdown induced-Golgi disruption. Dominant negative expression of a C-terminal fragment of Bicaudal D, a linker between Rab6 and dynactin/dynein, suppressed ZW10, but not COG, knockdown induced-Golgi disruption. We conclude that Rab6 regulates distinct Golgi trafficking pathways involving two separate protein complexes: ZW10/RINT-1 and COG.

Address correspondence to: Brian Storrie (StorrieBrian{at}uams.edu)




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A. Shestakova, E. Suvorova, O. Pavliv, G. Khaidakova, and V. Lupashin
Interaction of the conserved oligomeric Golgi complex with t-SNARE Syntaxin5a/Sed5 enhances intra-Golgi SNARE complex stability
J. Cell Biol., December 17, 2007; 179(6): 1179 - 1192.
[Abstract] [Full Text] [PDF]


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