Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print September 19, 2007
Mol. Biol. Cell 10.1091/mbc.E07-02-0127

A more recent version of this article appeared on December 1, 2007
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Materials
Right arrow All Versions of this Article:
E07-02-0127v1
18/12/4750    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Arden, S. D.
Right arrow Articles by Buss, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arden, S. D.
Right arrow Articles by Buss, F.

Submitted on February 15, 2007
Revised on August 24, 2007
Accepted on September 6, 2007

Myosin VI Is Required for Targeted Membrane Transport during Cytokinesis

Susan D. Arden,* Claudia Puri,* Josephine Sui-Yan Au,{dagger} John Kendrick-Jones,{dagger} and Folma Buss*

*Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom; {dagger}Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom

Monitoring Editor: Erika Holzbaur

Myosin VI plays important roles in endocytic and exocytic membrane trafficking pathways in cells. Because recent work has highlighted the importance of targeted membrane transport during cytokinesis, we investigated whether myosin VI plays a role in this process during cell division. In dividing cells myosin VI undergoes dramatic changes in localization: in prophase myosin VI is recruited to the spindle poles and in cytokinesis myosin VI is targeted to the walls of the ingressing cleavage furrow with a dramatic concentration in the midbody region. Furthermore, myosin VI is present on vesicles moving into and out of the cytoplasmic bridge connecting the two daughter cells. Inhibition of myosin VI activity by siRNA mediated knock down or by overexpression of dominant negative myosin VI tail leads to a delay in metaphase progression and a defect in cytokinesis. GIPC, a myosin VI binding partner, is associated with myosin VI’s function(s) in dividing cells. Loss of GIPC in siRNA knock down cells results in a more than fourfold increase in the number of multinucleated cells. Our results suggest that myosin VI has novel functions in mitosis and plays an essential role in targeted membrane transport during cytokinesis.

Address correspondence to: Folma Buss (fb1{at}mole.bio.cam.ac.uk)




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
M. A. Conti and R. S. Adelstein
Nonmuscle myosin II moves in new directions
J. Cell Sci., January 1, 2008; 121(1): 11 - 18.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2007 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.