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MBC in Press, published online ahead of print January 2, 2008
Mol. Biol. Cell 10.1091/mbc.E07-02-0130

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Submitted on February 15, 2007
Revised on November 29, 2007
Accepted on December 20, 2007

Dynamic Rearrangement of Nucleoporins During Fungal "Open" Mitosis

Ulrike Theisen,* Anne Straube,{dagger} and Gero Steinberg*

Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany

Monitoring Editor: Fred Chang

Mitosis in animals starts with the disassembly of the nuclear pore complexes and the breakdown of the nuclear envelope. In contrast to many fungi, the corn smut fungus Ustilago maydis also removes the nuclear envelope. Here we report on the dynamic behavior of the nucleoporins Nup214, Pom152, Nup133 and Nup107 in this "open" fungal mitosis. In prophase, the nuclear pore complexes disassembled and Nup214 and Pom152 dispersed in the cytoplasm and in the endoplasmic reticulum, respectively. Nup107 and Nup133 initially spread throughout the cytoplasm, but in metaphase and early anaphase appeared on the chromosomes. In anaphase, the Nup107-subcomplex redistributed to the edge of the chromosome masses, where the new envelope was reconstituted. Subsequently, Nup214 and Pom152 are recruited to the nuclear pores and protein import starts. Recruitment of nucleoporins and protein import reached a steady state in G2-phase. Formation of the nuclear envelope and assembly of nuclear pores occurred in the absence of microtubules or F-actin, but not if both were disrupted. Thus, the basic principles of nuclear pore complex dynamics appear to be conserved in organisms displaying "open" mitosis.

Present addresses: {dagger}Cytoskeletal Organization Laboratory, Marie Curie Research Institute, Trevereux Hill, Oxted, RH8 0TL, United Kingdom; *School of Bioscience, Stocker Rd., Exeter University, EX4 4QD, United Kingdom.

Address correspondence to: Gero Steinberg (G.Steinberg{at}exeter.ac.uk)




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K. R. Katsani, R. E. Karess, N. Dostatni, and V. Doye
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Mol. Biol. Cell, September 1, 2008; 19(9): 3652 - 3666.
[Abstract] [Full Text] [PDF]


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