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MBC in Press, published online ahead of print August 1, 2007
Mol. Biol. Cell 10.1091/mbc.E07-05-0474

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Submitted on May 21, 2007
Revised on July 20, 2007
Accepted on July 25, 2007

Proper Recruitment of {gamma}-Tubulin and D-TACC/Msps to Embryonic Drosophila Centrosomes Requires Centrosomin Motif 1

Jiuli Zhang and Timothy L. Megraw

Department of Pharmacology and The Cecil and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9051

Monitoring Editor: Yixian Zheng

Centrosomes are microtubule-organizing centers and play a dominant role in assembly of the microtubule spindle apparatus at mitosis. Although the individual binding steps in centrosome maturation are largely unknown, Centrosomin is an essential mitotic centrosome component required for assembly of all other known pericentriolar (PCM) proteins to achieve microtubule-organizing activity at mitosis in Drosophila. We have identified a conserved motif (Motif 1) near the amino terminus of Centrosomin that is essential for its function in vivo. Centrosomin Motif 1 is necessary for proper recruitment of {gamma}-Tubulin, D-TACC and Msps to embryonic centrosomes but is not required for assembly of other centrosome components including Aurora A kinase and CP60. Centrosome separation and centrosomal satellite formation are severely disrupted in Centrosomin Motif 1 mutant embryos. However, actin organization into pseudocleavage furrows, though aberrant, remains partially intact. These data show that Motif 1 is necessary for some but not all of the activities conferred on centrosome function by intact Centrosomin.

Address correspondence to: Timothy L. Megraw (timothy.megraw{at}utsouthwestern.edu)




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