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MBC in Press, published online ahead of print December 19, 2007
Mol. Biol. Cell 10.1091/mbc.E07-06-0600

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Submitted on June 22, 2007
Revised on November 6, 2007
Accepted on December 10, 2007

A Lipid-mediated Quality Control Process in the Golgi Apparatus in Yeast

Ludovic Pineau,* Laetitia Bonifait,{dagger} Jean-Marc Berjeaud,{dagger} Parissa Alimardani-Theuil,{ddagger} Thierry Bergès,* and Thierry Ferreira*

*Unité Mixte de Recherche Centre National de la Recherche Scientifique 6161 "Transport des assimilats" and {dagger}Unité Mixte de Recherche Centre National de la Recherche Scientifique 6008 "Microbiologie fondamentale et appliquée," Université de Poitiers, 86022 Poitiers, France

Monitoring Editor: Sean Munro

When haem biosynthesis is disrupted, the yeast S. cerevisiae becomes unable to synthesize its major sterol, ergosterol, and desaturate fatty acids. We took advantage of this physiological peculiarity to evaluate the consequences of ergosterol and/or unsaturated fatty acid (UFA) depletions on the biogenesis of a model polytopic plasma membrane protein, the uracil permease Fur4p. We show that under UFA shortage, which results in low amounts of diunsaturated phospholipid species, as well as under ergosterol depletion, Fur4p is prematurely routed from the Golgi apparatus to the vacuolar lumen in a process that requires the ubiquitin ligase Rsp5p. Interestingly, this diversion is not correlated to Fur4p exclusion from Detergent-Resistant Membranes. In an independent set of experiments, we show that Fur4p targeting to the plasma membrane depends on phosphatidylethanolamine amounts and more specifically on the propensity of this phospholipid to form a hexagonal phase. In light of recent literature, we propose a model in which ergosterol and diunsaturated phospholipid species maintain optimal membrane curvature for Fur4p to evade the Golgi quality control process and to be properly delivered to its normal destination.

{ddagger}Present address: Université de Reims, Faculté des Sciences, "Laboratoire de microbiologie générale et moléculaire," Moulin de la Housse, BP 1039, 51687 Reims Cedex 2, France.

Address correspondence to: Thierry Ferreira (thierry.ferreira{at}univ-poitiers.fr)






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