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MBC in Press, published online ahead of print November 14, 2007
Mol. Biol. Cell 10.1091/mbc.E07-08-0801

A more recent version of this article appeared on January 1, 2008 Originally published as MBC in Press, 10.1091/mbc.E07-08-0801 on November 15, 2007
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Submitted on August 19, 2007
Revised on October 25, 2007
Accepted on November 2, 2007

Distinct Dgrip84 Isoforms Correlate with Distinct {gamma}-Tubulins in Drosophila

Christiane Wiese

Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706

Monitoring Editor: Sandra Schmid

{gamma}-Tubulin is an indispensable component of the animal centrosome and is required for proper microtubule organization. Within the cell, {gamma}-tubulin exists in a multi-protein complex containing between two (some yeasts) and six or more (metazoa) additional highly conserved proteins named gamma ring proteins (Grips) or gamma complex proteins (GCPs). {gamma}-Tubulin containing complexes isolated from Xenopus eggs or Drosophila embryos appear ring-shaped and have therefore been named the {gamma}-tubulin ring complex ({gamma}TuRC). Curiously, many organisms (including humans) have two distinct {gamma}-tubulin genes. In Drosophila, where the two {gamma}-tubulin isotypes have been studied most extensively, the {gamma}-tubulin genes are developmentally regulated: the ‘maternal’ {gamma}-tubulin isotype (named {gamma}Tub37CD according to its location on the genetic map) is expressed in the ovary and is deposited in the egg, where it is thought to orchestrate the meiotic and early embryonic cleavages. The second {gamma}-tubulin isotype ({gamma}Tub23C) is ubiquitously expressed and persists in most of the cells of the adult fly. In those rare cases where both {gamma}-tubulins coexist in the same cell, they show distinct subcellular distributions and cell-cycle-dependent changes (Raynaud-Messina et al., 2001. Eur. J. Cell Biol. 80, 643–649): {gamma}Tub37CD mainly localizes to the centrosome, where its levels vary only slightly with the cell cycle. In contrast, the level of {gamma}Tub23C at the centrosome increases at the beginning of mitosis, and {gamma}Tub23C also associates with spindle pole microtubules. Here, we show that {gamma}Tub23C forms discrete complexes that closely resemble the complexes formed by {gamma}Tub37CD. Surprisingly, however, {gamma}Tub23C associates with a distinct, longer splice variant of Dgrip84. This may reflect a role for Dgrip84 in regulating the activity and/or the location of the {gamma}-tubulin complexes formed with {gamma}Tub37CD and {gamma}Tub23C.

Address correspondence to: Christiane Wiese (wiese{at}biochem.wisc.edu)




This article has been cited by other articles:


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M. Vazquez, M. T. Cooper, M. Zurita, and J. A. Kennison
{gamma}Tub23C Interacts Genetically With Brahma Chromatin-Remodeling Complexes in Drosophila melanogaster
Genetics, October 1, 2008; 180(2): 835 - 843.
[Abstract] [Full Text] [PDF]


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