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MBC in Press, published online ahead of print November 28, 2007
Mol. Biol. Cell 10.1091/mbc.E07-08-0847

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Submitted on August 30, 2007
Revised on November 18, 2007
Accepted on November 20, 2007

The Golgi Protein GM130 Regulates Centrosome Morphology and Function

Andrew Kodani and Christine Sütterlin

Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92697-2300

Monitoring Editor: Vivek Malhotra

The Golgi apparatus (GA) of mammalian cells is positioned in the vicinity of the centrosome, the major microtubule organizing center of the cell. The significance of this physical proximity for organelle function and cell cycle progression is only beginning to being understood. We have identified a novel function for the GA protein, GM130, in the regulation of centrosome morphology, position and function during interphase. RNAi-mediated depletion of GM130 from five human cell lines revealed abnormal interphase centrosomes that were mispositioned and defective with respect to microtubule organization and cell migration. When GM130-depleted cells entered mitosis, they formed multipolar spindles, arrested in metaphase and died. We also detected aberrant centrosomes during interphase and multipolar spindles during mitosis in ldlG cells, which do not contain detectable GM130. While GA proteins have been described to regulate mitotic centrosomes and spindle formation, this is the first report of a role for a GA protein in the regulation of centrosomes during interphase.

Address correspondence to: Christine Sütterlin (suetterc{at}uci.edu)




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