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MBC in Press, published online ahead of print April 2, 2008
Mol. Biol. Cell 10.1091/mbc.E07-09-0862

A more recent version of this article appeared on June 1, 2008
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Submitted on September 6, 2007
Revised on March 18, 2008
Accepted on March 24, 2008

RAB-11 Permissively Regulates Spindle Alignment by Modulating Metaphase Microtubule Dynamics in C. elegans Early Embryos

Haining Zhang,*{dagger} Jayne M. Squirrell,{dagger} and John G. White{dagger}{ddagger}

*Laboratory of Genetics, {dagger}Laboratory of Molecular Biology {ddagger}Department of Anatomy, University of Wisconsin, Madison, WI 53706

Monitoring Editor: Sean Munro

Alignment of the mitotic spindle along a pre-formed axis of polarity is crucial for generating cell diversity in many organisms (Segal and Bloom, 2001; Betschinger and Knoblich, 2004), yet little is known about the role of the endomembrane system in this process. RAB-11 is a small GTPase enriched in recycling endosomes. When we depleted RAB-11 by RNAi in C. elegans, the spindle of the one-cell embryo failed to align along the axis of polarity in metaphase and underwent violent movements in anaphase. The distance between astral microtubules ends and the anterior cortex was significantly increased in rab-11(RNAi) embryos specifically during metaphase, possibly accounting for the observed spindle alignment defects. Additionally, we found that normal ER morphology requires functional RAB-11, particularly during metaphase. We hypothesize that RAB-11, in conjunction with the ER, acts to regulate cell cycle specific changes in astral microtubule length to ensure proper spindle alignment in C. elegans early embryos.

Address correspondence to: John G. White (jwhite1{at}wisc.edu)




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