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MBC in Press, published online ahead of print February 6, 2008
Mol. Biol. Cell 10.1091/mbc.E07-09-0910

A more recent version of this article appeared on April 1, 2008
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Submitted on September 17, 2007
Revised on January 17, 2008
Accepted on January 30, 2008

Sister Kinetochore Recapture in Fission Yeast Occurs by Two Distinct Mechanisms, Both Requiring Dam1 and Klp2

Yannick Gachet,* Céline Reyes,* Thibault Courthéoux, Sherilyn Goldstone, Guillaume Gay, Céline Serrurier, and Sylvie Tournier

Laboratoire de Biologie Cellulaire et Moléculaire du Controle de la Prolifération (LBCMCP), CNRS, Université de Toulouse, 118 route de Narbonne, 31062 Toulouse, France

Monitoring Editor: David Drubin

In eukaryotic cells, proper formation of the spindle is necessary for successful cell division. We have studied chromosome recapture in the fission yeast Schizosaccharomyces pombe. We show by live cell analysis that lost kinetochores interact laterally with intranuclear microtubules (INMs) and that both microtubule depolymerization (end-on pulling) and minus-end directed movement (microtubule sliding) contribute to chromosome retrieval to the SPB. We find that the minus-end directed motor Klp2 colocalizes with the kinetochore during its transport to the SPB and contributes to the effectiveness of retrieval by affecting both end-on pulling and lateral sliding. Furthermore, we provide in vivo evidence that Dam1, a component of the DASH complex, also colocalizes with the kinetochore during its transport and is essential for its retrieval by either of these mechanisms. Finally, we find that the position of the unattached kinetochore correlates with the size and orientation of the INMs, suggesting that chromosome recapture may not be a random process.

*These authors contributed equally to this work.

Address correspondence to: Sylvie Tournier (tournier{at}cict.fr)




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S. Saitoh, Y. Kobayashi, Y. Ogiyama, and K. Takahashi
Dual Regulation of Mad2 Localization on Kinetochores by Bub1 and Dam1/DASH that Ensure Proper Spindle Interaction
Mol. Biol. Cell, September 1, 2008; 19(9): 3885 - 3897.
[Abstract] [Full Text] [PDF]


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