Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print July 23, 2008
Mol. Biol. Cell 10.1091/mbc.E07-09-0946

A more recent version of this article appeared on October 1, 2008
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Materials
Right arrow All Versions of this Article:
E07-09-0946v1
19/10/4393    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ramsdale, M.
Right arrow Articles by Brown, A. J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramsdale, M.
Right arrow Articles by Brown, A. J. P.

Submitted on September 21, 2007
Revised on June 30, 2008
Accepted on July 11, 2008

MNL1 Regulates Weak Acid-induced Stress Responses of the Fungal Pathogen Candida albicans

Mark Ramsdale,*{dagger} Laura Selway,* David Stead,* Jan Walker,* Zhikang Yin,* Susan M. Nicholls,* Jonathan Crowe,* Emma M. Sheils,* and Alistair J. P. Brown*

*Institute of Medical Sciences, School of Medical Sciences, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom; {dagger}School of Biosciences, University of Exeter, Exeter, Devon, EX4 4PS, United Kingdom

Monitoring Editor: Mark J. Solomon

MNL1, the Candida albicans homologue of an orphan Msn2-like gene (YER130c in S. cerevisiae) has no known function. Here we report that MNL1 regulates weak acid stress responses. Deletion of MNL1 prevents the long-term adaptation of C. albicans cells to weak acid stresses and compromises their global transcriptional response under these conditions. The promoters of Mnl1-dependent genes contain a novel STRE-like element (SLE) that imposes Mnl1-dependent, weak acid stress-induced transcription upon a lacZ reporter in C. albicans. The SLE (HHYYCCCCTTYTY) is related to the NRE element recognized by the transcriptional repressor Nrg1. Deletion of NRG1 partially restores the ability of C. albicans mnl1 cells to adapt to weak acid stress indicating that Mnl1 and Nrg1 act antagonistically to regulate this response. Molecular, microarray and proteomic analyses revealed that Mnl1-dependent adaptation does not occur in cells exposed to proapoptotic or pronecrotic doses of weak acid, suggesting that Ras-pathway activation might suppress the Mnl1-dependent weak acid response in dying cells. Our work defines a role for this YER130c orthologue in stress adaptation and cell death.

Address correspondence to: Mark Ramsdale (m.ramsdale{at}exeter.ac.uk)




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
A. Rodaki, I. M. Bohovych, B. Enjalbert, T. Young, F. C. Odds, N. A.R. Gow, and A. J.P. Brown
Glucose Promotes Stress Resistance in the Fungal Pathogen Candida albicans
Mol. Biol. Cell, November 15, 2009; 20(22): 4845 - 4855.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
Y.-S. Bahn
Master and Commander in Fungal Pathogens: the Two-Component System and the HOG Signaling Pathway
Eukaryot. Cell, December 1, 2008; 7(12): 2017 - 2036.
[Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2008 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.