Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print February 6, 2008
Mol. Biol. Cell 10.1091/mbc.E07-09-0950

A more recent version of this article appeared on April 1, 2008
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E07-09-0950v1
19/4/1763    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kono, K.
Right arrow Articles by Ohya, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kono, K.
Right arrow Articles by Ohya, Y.

Submitted on September 21, 2007
Revised on January 16, 2008
Accepted on January 30, 2008

G1/S Cyclin-Dependent Kinase Regulates Small GTPase Rho1p through Phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae

Keiko Kono,*{dagger} Satoru Nogami,* Mitsuhiro Abe,* Masafumi Nishizawa,{ddagger} Shinichi Morishita,{sect} David Pellman,{dagger} and Yoshikazu Ohya*

*Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan; {dagger}Department of Pediatric Oncology, Dana-Farber Cancer Institute and Division of Hematology/Oncology, Children’s Hospital Boston and Harvard Medical School, Boston, MA 02115; {ddagger}Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo 160-8582, Japan; {sect}Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan

Monitoring Editor: Mark Solomon

Rho1p is an essential small GTPase that plays a key role in the morphogenesis of S. cerevisiae. We show here that the activation of Rho1p is regulated by a cyclin-dependent kinase (CDK). Rho1p is activated at the G1/S transition at the incipient-bud sites by the Cln2p (G1 cyclin) and Cdc28p (CDK) complex, in a process mediated by Tus1p, a guanine nucleotide exchange factor for Rho1p. Tus1p interacts physically with Cln2p/Cdc28p and is phosphorylated in a Cln2p/Cdc28p-dependent manner. CDK phosphorylation consensus sites in Tus1p are required for both Cln2p-dependent activation of Rho1p and polarized organization of the actin cytoskeleton. We propose that Cln2p/Cdc28p-dependent phosphorylation of Tus1p is required for appropriate temporal and spatial activation of Rho1p at the G1/S transition.

Address correspondence to: Yoshikazu Ohya (ohya{at}k.u-tokyo.ac.jp)




This article has been cited by other articles:


Home page
 Eukaryot CellHome page
H.-L. Ho, H.-Y. Lee, H.-C. Liao, and M.-Y. Chen
Involvement of Saccharomyces cerevisiae Avo3p/Tsc11p in Maintaining TOR Complex 2 Integrity and Coupling to Downstream Signaling
Eukaryot. Cell, August 1, 2008; 7(8): 1328 - 1343.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2008 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.