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MBC in Press, published online ahead of print January 30, 2008
Mol. Biol. Cell 10.1091/mbc.E07-10-1015

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Submitted on October 9, 2007
Revised on January 7, 2008
Accepted on January 18, 2008

Inactivation of Cleavage Factor I Components Rna14p and Rna15p Induces Sequestration of snoRNPs at Discrete Sites in the Nucleus

Tiago Carneiro,*{dagger} Célia Carvalho,* José Braga,* José Rino,* Laura Milligan,{ddagger} David Tollervey,{ddagger} and Maria Carmo-Fonseca*

*Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal; {ddagger}Wellcome Trust Centre for Cell Biology, University of Edinburgh, EH9 3JR, United Kingdom

Monitoring Editor: A. Gregory Matera

Small nucleolar RNAs (snoRNAs) associate with specific proteins forming small nucleolar ribonucleoprotein (snoRNP) particles, which are essential for ribosome biogenesis. The snoRNAs are transcribed, processed and assembled in snoRNPs in the nucleoplasm. Mature particles are then transported to the nucleolus. In yeast, 3'-end maturation of snoRNAs involves the activity of Rnt1p endonuclease and cleavage factor IA (CFIA). We report that following inhibition of CFIA components Rna14p and Rna15p, the snoRNP proteins Nop1p, Nop58p and Gar1p delocalise from the nucleolus and accumulate in discrete nucleoplasmic foci. The U14 snoRNA, but not U3 snoRNA, similarly redistributes from the nucleolus to the nucleoplasmic foci. Simultaneous depletion of either Rna14p or Rna15p and the nuclear exosome component Rrp6p induces accumulation of poly(A)+ RNA at the snoRNP-containing foci. We propose that the foci detected after CFIA inactivation correspond to quality control centers in the nucleoplasm.

{dagger}Present address: Instituto Gulbenkian de Ciência, Oeiras, Portugal.

Address correspondence to: Maria Carmo-Fonseca (carmo.fonseca{at}fm.ul.pt)






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