Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print February 20, 2008
Mol. Biol. Cell 10.1091/mbc.E07-10-1048

A more recent version of this article appeared on May 1, 2008
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E07-10-1048v1
19/5/2039    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kawamata, T.
Right arrow Articles by Ohsumi, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kawamata, T.
Right arrow Articles by Ohsumi, Y.

Submitted on October 18, 2007
Revised on February 7, 2008
Accepted on February 13, 2008

Organization of the Pre-Autophagosomal Structure Responsible for Autophagosome Formation

Tomoko Kawamata,*{dagger} Yoshiaki Kamada,* Yukiko Kabeya, Takayuki Sekito, and Yoshinori Ohsumi

Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan; School of Life Science, The Graduate University for Advanced Studies, Okazaki 444-8585, Japan

Monitoring Editor: Suresh Subramani

Autophagy induced by nutrient depletion is involved in survival during starvation conditions. In addition to starvation-induced autophagy, the yeast S. cerevisiae also has a constitutive autophagy-like system, the Cvt pathway. Among 31 Atg (autophagy-related) proteins, the function of Atg17, Atg29, and Atg31 are required specifically for autophagy. In this study, we investigated the role of autophagy–specific (i.e., nonCvt) proteins under autophagy-inducing conditions. For this purpose, we used atg11{Delta} cells in which the Cvt pathway is abrogated. The autophagy-unique proteins are required for the localization of Atg proteins to the pre-autophagosomal structure (PAS), the putative site for autophagosome formation, under starvation condition. It is likely that these Atg proteins function as a ternary complex, because Atg29 and Atg31 bind to Atg17. The Atg1 kinase complex (Atg1-Atg13) is also essential for recruitment of Atg proteins to the PAS. The assembly of Atg proteins to the PAS is observed only under autophagy-inducing conditions, indicating that this structure is specifically involved in autophagosome formation. Our results suggest that Atg1 complex and the autophagy-unique Atg proteins cooperatively organize the PAS in response to starvation signals.

*These authors contributed equally to this work.

{dagger}Present address: Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.

Address correspondence to: Yoshinori Ohsumi (yohsumi{at}nibb.ac.jp)




This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
Y. Cao, H. Cheong, H. Song, and D. J. Klionsky
In vivo reconstitution of autophagy in Saccharomyces cerevisiae
J. Cell Biol., August 26, 2008; 182(4): 703 - 713.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
T. Hara, A. Takamura, C. Kishi, S.-i. Iemura, T. Natsume, J.-L. Guan, and N. Mizushima
FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells
J. Cell Biol., May 1, 2008; 181(3): 497 - 510.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2008 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.