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MBC in Press, published online ahead of print May 7, 2008
Mol. Biol. Cell 10.1091/mbc.E07-11-1156

A more recent version of this article appeared on July 1, 2008
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Submitted on November 19, 2007
Revised on April 16, 2008
Accepted on April 29, 2008

High Resolution Crystal Structure and in vivo Function of a Kinesin-2 Homolog in Giardia intestinalis

J. C. Hoeng,*{dagger} S. C. Dawson,{dagger}{ddagger} S. A. House,{ddagger} M. S. Sagolla,{sect} J. K. Pham,{ddagger} J. J. Mancuso,{sect} J. Löwe,* and W.Z. Cande{sect}

*Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom; {ddagger}Section of Microbiology, University of California, Davis, Davis, CA 95616; {sect}Deptartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3200

Monitoring Editor: Tim Stearns

A critical component of flagellar assembly, the kinesin-2 heterotrimeric complex powers the anterograde movement of proteinaceous rafts along the outer doublet of axonemes in intraflagellar transport (IFT). We present the first high-resolution structures of a kinesin-2 motor domain and an ATP hydrolysis deficient motor domain mutant from the parasitic protist Giardia intestinalis. The high-resolution crystal structures of Giardia intestinalis wild-type kinesin-2 (GiKIN2a) motor domain with its docked neck linker and the hydrolysis deficient mutant GiKIN2aT104N were solved in a complex with ADP and Mg2+ at 1.6 Å and 1.8 Å resolutions, respectively. These high-resolution structures provide unique insight into the nucleotide coordination within the active site. Giardia intestinalis has eight flagella, and we demonstrate that both kinesin-2 homologues and IFT proteins localize to both cytoplasmic and membrane-bound regions of axonemes with foci at cell body exit points and the distal flagellar tips. We demonstrate that the T104N mutation causes GiKIN2a to act as a rigor mutant in vitro. Overexpression of GiKIN2aT104N results in significant inhibition of flagellar assembly in the caudal, ventral, and posterolateral flagellar pairs. Thus we confirm the conserved evolutionary structure and functional role of kinesin-2 as the anterograde IFT motor in Giardia intestinalis.

{dagger}These authors contributed equally to this work. S. C. Dawson (scdawson{at}ucdavis.edu)






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