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MBC in Press, published online ahead of print April 16, 2008
Mol. Biol. Cell 10.1091/mbc.E07-11-1199

A more recent version of this article appeared on July 1, 2008
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Submitted on December 1, 2007
Revised on March 10, 2008
Accepted on April 9, 2008

Analysis of the Yeast Kinome Reveals a Network of Regulated Protein Localization During Filamentous Growth

Nikë Bharucha, Jun Ma, Craig J. Dobry, Sarah K. Lawson, Zhifen Yang, and Anuj Kumar

Department of Molecular, Cellular, and Developmental Biology, and Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109-2216

Monitoring Editor: Charles Boone

The subcellular distribution of kinases and other signaling proteins is regulated in response to cellular cues; however, the extent of this regulation has not been investigated for any gene set in any organism. Here, we present a systematic analysis of protein kinases in the budding yeast, screening for differential localization during filamentous growth. Filamentous growth is an important stress response involving MAPK and PKA signaling modules, wherein yeast cells form interconnected and elongated chains. As standard strains of yeast are nonfilamentous, we constructed a unique set of 125 kinase-YFP chimeras in the filamentous {Sigma}1278b strain for this study. In total, we identified six cytoplasmic kinases (Bcy1p, Fus3p, Ksp1p, Kss1p, Sks1p, Tpk2p) that localize predominantly to the nucleus during filamentous growth. These kinases form part of an interdependent, localization-based regulatory network: deletion of each individual kinase, or loss of kinase activity, disrupts the nuclear translocation of at least two other kinases. In particular, this study highlights a previously unknown function for the kinase Ksp1p, indicating the essentiality of its nuclear translocation during yeast filamentous growth. Thus, the localization of Ksp1p and the other kinases identified here is tightly controlled during filamentous growth, representing an overlooked regulatory component of this stress response.

Address correspondence to: Anuj Kumar (anujk{at}umich.edu)






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