Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print May 14, 2008
Mol. Biol. Cell 10.1091/mbc.E08-01-0035

A more recent version of this article appeared on July 1, 2008
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Materials
Right arrow All Versions of this Article:
E08-01-0035v1
19/7/3147    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, Y.-C. M.
Right arrow Articles by Spector, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, Y.-C. M.
Right arrow Articles by Spector, D. L.

Submitted on January 16, 2008
Revised on April 22, 2008
Accepted on May 1, 2008

Live Cell Dynamics of PML Nuclear Bodies upon Entry into and Exit from Mitosis

Yi-Chun M. Chen,*{dagger} Constantin Kappel,{ddagger} Joel Beaudouin,{sect} Roland Eils,{ddagger}{sect} and David L. Spector*{dagger}

*Molecular and Cellular Biology Program, Stony Brook University, Stony Brook, NY 11794; {dagger}Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724; {ddagger}Division of Theoretical Bioinformatics, German Cancer Research Center, D-69120 Heidelberg, Germany; {sect}Institute for Pharmacy and Molecular Biotechnology, University of Heidelberg, D-69120 Heidelberg, Germany

Monitoring Editor: A. Gregory Matera

Promyelocytic leukemia nuclear bodies (PML NBs) have been proposed to be involved in tumor suppression, viral defense, DNA repair, and/or transcriptional regulation. To study the dynamics of PML NBs during mitosis, we developed several U2OS cell lines stably coexpressing PML-ECFP with other individual marker proteins. Using three-dimensional time lapse live cell imaging and 4D particle tracking, we quantitatively demonstrated that PML NBs exhibit a high percentage of directed movement when cells progressed from prophase to prometaphase. The timing of this increased dynamic movement occurred just before or upon nuclear entry of cyclin B1, but before nuclear envelope breakdown. Our data suggests that entry into prophase leads to a loss of tethering between regions of chromatin and PML NBs resulting in their increased dynamics. On exit from mitosis, Sp100 and Daxx entered the daughter nuclei after a functional nuclear membrane was reformed. However, the recruitment of these proteins to PML NBs was delayed and correlated with the timing of de novo PML NB formation. Together, these results provide insight into the dynamic changes associated with PML NBs during mitosis.

Address correspondence to: David L. Spector (spector{at}cshl.edu)






Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2008 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.