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MBC in Press, published online ahead of print March 26, 2008
Mol. Biol. Cell 10.1091/mbc.E08-02-0118

A more recent version of this article appeared on June 1, 2008
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Submitted on February 5, 2008
Revised on March 17, 2008
Accepted on March 19, 2008

Meiotic Spindle Pole Bodies Acquire the Ability to Assemble the Spore Plasma Membrane by Sequential Recruitment of Sporulation-specific Components in Fission Yeast

Yukiko Nakase,*{dagger} Michiko Nakamura-Kubo,* Yanfang Ye,* Aiko Hirata,{ddagger} Chikashi Shimoda,* and Taro Nakamura*

*Department of Biology, Graduate School of Science, Osaka City University, Osaka 558-8585, Japan; {ddagger}Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba 277-8562, Japan

Monitoring Editor: Patrick Brennwald

The spindle pole body (SPB) of Schizosaccharomyces pombe is required for assembly of the forespore membrane (FSM) during meiosis. Before de novo biogenesis of the FSM, the meiotic SPB forms outer plaques, an event referred to as SPB modification. A constitutive SPB component, Spo15, plays an indispensable role in SPB modification and sporulation. Here, we analyzed two sporulation-specific genes, spo13+ and spo2+, which are not required for progression of meiotic nuclear divisions, but are essential for sporulation. Spo13 is a 16 kDa coiled-coil protein, and Spo2 is a 15 kDa nonconserved protein. Both Spo13 and Spo2 specifically associated with the meiotic SPB. The respective deletion mutants are viable, but defective in SPB modification and in the onset of FSM formation. Spo13 and Spo2 localized on the cytoplasmic side of the SPB in close contact with the nascent FSM. Localization of Spo13 to the SPB was dependent on Spo15 and Spo2; that of Spo2 depended only on Spo15, suggesting that their recruitment to the SPB is strictly controlled. Spo2 physically associated with both Spo15 and Spo13, but Spo13 and Spo15 did not interact directly. Taken together, these observations indicate that Spo2 is recruited to the SPB during meiosis, and then assists in the localization of Spo13 to the outer surface of the SPB.

{dagger}Present address: Radiation Biology Center, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.

Address correspondence to: Taro Nakamura (taronaka{at}sci.osaka-cu.ac.jp)




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