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MBC in Press, published online ahead of print January 14, 2009
Mol. Biol. Cell 10.1091/mbc.E08-10-1000

A more recent version of this article appeared on March 1, 2009
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Submitted on October 7, 2008
Revised on December 8, 2008
Accepted on January 5, 2009

Reengineering Ribosome Export

Kai-Yin Lo and Arlen W. Johnson

Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, the University of Texas at Austin, Austin, TX, 78712

Monitoring Editor: Karsten Weis

Large cargoes require multiple receptors for efficient transport through the nuclear pore complex. The 60S ribosomal subunit is one of the bulkiest transport cargoes, and in yeast three different receptors, Crm1, Mex67/Mtr2 and Arx1, collaborate in its export. However, only Crm1, recruited by the adapter Nmd3, appears to be conserved for 60S export in higher eukaryotes. We asked if export of the large subunit requires specific receptors. We made protein fusions between mutant Nmd3 and various export receptors. Surprisingly, fusions of Mex67, the tRNA exportin Los1, Mtr2, Cse1, or Msn5 to Nmd3, lacking its Crm1-dependent nuclear export signal (NES), all functioned in export. Furthermore, these chimeric proteins supported 60S export even in the presence of the Crm1 inhibitor leptomycin B, indicating that export was now independent of Crm1. These results suggest that there is not a requirement for a specific export receptor for the large subunit, as recruitment of any receptor will suffice. Finally we show that the addition of an NES directly to the 60S ribosomal subunit protein Rpl3 promotes export. These results imply remarkable flexibility in the export pathway for the 60S subunit and help explain how different export receptors could have evolved in different eukaryotic lineages.

Address correspondence to: Arlen W. Johnson (arlen{at}mail.utexas.edu)




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J. Merl, S. Jakob, K. Ridinger, T. Hierlmeier, R. Deutzmann, P. Milkereit, and H. Tschochner
Analysis of ribosome biogenesis factor-modules in yeast cells depleted from pre-ribosomes
Nucleic Acids Res., January 25, 2010; (2010): gkp1244v1 - gkp1244.
[Abstract] [Full Text] [PDF]


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