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MBC in Press, published online ahead of print April 8, 2009
Mol. Biol. Cell 10.1091/mbc.E08-12-1253

A more recent version of this article appeared on June 1, 2009
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Submitted on January 2, 2009
Revised on March 12, 2009
Accepted on March 27, 2009

Kinesin-1 and Cytoplasmic Dynein Act Sequentially to Move the Meiotic Spindle to the Oocyte Cortex in C. elegans

Marina L. Ellefson and Francis J. McNally

Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616

Monitoring Editor: Tim Stearns

During female meiosis in animals, the meiotic spindle is attached to the egg cortex by one pole during anaphase to allow selective disposal of half the chromosomes in a polar body. In C. elegans, this anaphase spindle position is achieved sequentially through kinesin-1-dependent early translocation followed by anaphase promoting complex (APC)-dependent spindle rotation. Partial depletion of cytoplasmic dynein heavy chain by RNAi blocked spindle rotation without affecting early translocation. Dynein depletion also blocked the APC-dependent late translocation that occurs in kinesin-1-depleted embryos. Time-lapse imaging of GFP-tagged dynein heavy chain as well as immunofluorescence with dynein-specific antibodies revealed that dynein starts to accumulate at spindle poles just before the initiation of rotation or late translocation. Accumulation of dynein at poles was kinesin-1-independent and APC-dependent, just like dynein driven spindle movements. This represents a case of kinesin-1/dynein coordination in which these two motors of opposite polarity act sequentially and independently on a cargo to move it in the same direction.

Address correspondence to: Francis J. McNally (fjmcnally{at}ucdavis.edu)






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