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This item has the following additional materials available:
All videos were acquired and processed as described previously in the Methods section.
video1.mov Confocal microscopy imaging of GFP-clathrin in transfected MDCK cells. Notice the budding of GFP-clathrin-coated intermediates from the TGN and their translocation towards the peripheral cytoplasm (arrows).
video2.mov Confocal microscopy imaging of GFP-GGA1 in transfected MDCK cells. Notice the budding of GFP-GGA1-coated intermediates from the TGN and their translocation towards the peripheral cytoplasm (arrows).
video_3.mov Confocal microscopy imaging of GFP-AP-1 in transfected MDCK cells. Notice the budding of GFP-AP-1-coated intermediates from the TGN and their translocation towards the peripheral cytoplasm (arrows).
video_A.mov Fluorescence recovery after photobleaching of GFP-clathrin in MDCK cells at 37°C.
video_B.mov Fluorescence recovery after photobleaching of GFP-GGA1 in MDCK cells at 37°C.
video_C.mov Fluorescence recovery after photobleaching of GFP-GGA1 in MDCK cells at 37°C. Note that the GFP-GGA1 recovers on the same TGN structures where it was located prior to photobleaching.
Prepared by: the MBC Journal Production Manager
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