INTRODUCTION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERNCES

Many mitochondrial precursor proteins are synthesized with amino-terminal targeting sequences, termed presequences, that direct the proteins to the organelle and across the outer and inner membranes (Ryan and Jensen, 1995; Schatz and Dobberstein, 1996; Neupert, 1997; Pfanner et al., 1997; Ryan and Pfanner, 1998). In the matrix, the presequences are typically cleaved off by the mitochondrial processing peptidase. A number of mitochondrial preproteins are not synthesized with cleavable targeting signals. A few preproteins were shown to contain the targeting information at the amino-terminal portion of the protein that is to carry a "noncleaved presequence" (Hurt et al., 1985; Arakawa et al., 1990; Rospert et al., 1993; Hahne et al., 1994; Jarvis et al., 1995); however, most noncleavable preproteins, notably those that are membrane proteins, seem to contain internal targeting information distributed over various regions of the preprotein (Pfanner et al., 1987a; Smagula and Douglas, 1988; Davis et al., 1998; Káldi et al., 1998). Typical representatives are the members of the large family of inner membrane metabolite carriers, such as the ADP/ATP carrier (AAC).

The mitochondrial machinery for the import of presequence-containing preproteins has been studied in detail. The presequences are typically recognized by the surface receptor Tom20, the 20-kDa subunit of the translocase of the outer membrane (Söllner et al., 1989; Ramage et al., 1993; Brix et al., 1997). Subsequently, the preproteins are transferred to the general import pore (GIP) complex where they interact with Tom22 and Tom5 and are translocated through the import channel formed by Tom40 (Vestweber et al., 1989; Kiebler et al., 1993; Dietmeier et al., 1997; Hill et al., 1998). The presequences bind to Tim23 of the translocase of the inner membrane and in a membrane potential ()-dependent reaction move through the inner membrane channel, that is, the Tim core complex formed by Tim23 and Tim17 (Dekker et al., 1993, 1997; Emtage and Jensen, 1993; Ryan et al., 1994; Bauer et al., 1996). Matrix-located heat shock protein 70 (mtHsp70) cooperates with Tim44 to drive the completion of preprotein translocation (Kronidou et al., 1994; Rassow et al., 1994; Schneider et al., 1994; Voos et al., 1996; Bömer et al., 1998).

The inner membrane carriers follow a different import route that converges with the presequence pathway only at the level of the GIP complex. The carrier preproteins such as AAC are preferentially recognized by Tom70 before their transfer to the GIP (Hines et al., 1990; Söllner et al., 1990). At the trans side of the outer membrane, the presequence and carrier pathways diverge (Moczko et al., 1997; Kübrich et al., 1998). Recent studies led to the identification of a number of new Tim proteins that mediate the translocation through the intermembrane space and insertion into the inner membrane. Three homologous small Tim proteins of the intermembrane space, Tim9, Tim10, and Tim12, bind the carrier preproteins (Koehler et al., 1998a,b; Sirrenberg et al., 1998) and transfer them to a translocase of the inner membrane that contains Tim22 and Tim54 (Sirrenberg et al., 1996; Kerscher et al., 1997). The carrier proteins contain six membrane-spanning segments each and are inserted into the inner membrane in a -dependent manner via Tim22-Tim54. Tim23, Tim17, and Tim22 contain a homologous membrane domain with four predicted membrane-spanning segments, and recent evidence indicates that Tim23 and most likely Tim17 and Tim22 are also imported via the carrier Tim pathway (Kerscher et al., 1997; Káldi et al., 1998).

Little is known about the actual biogenesis of the Tim proteins that are involved in the import of carrier preproteins. For this study, we have analyzed targeting and translocation of the precursors of these Tim proteins. We report that the targeting pathways of Tim22 and Tim54 reveal a new principle of combination of different portions of the main (presequence) pathway and the special (carrier) pathway. The crossing over occurs at the level of the GIP complex. Moreover, import of the small Tims provides the first example for a preferential targeting via Tom5 and not via the trypsin-accessible domains of the larger receptors.

	MATERIALS AND METHODS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERNCES

Construction of Plasmids for in Vitro Transcription

The open reading frames of yeast TIM54 (Kerscher et al., 1997), TIM22 (Sirrenberg et al., 1996), TIM13 (Accession No. P53299), TIM12 (Jarosch et al., 1996), TIM10 (Jarosch et al., 1997), TIM9 (Koehler et al., 1998b), and TIM8 (Accession No. Y13136) were amplified by PCR and individually cloned into pGEM-4Z (Promega, Madison, WI). Tim54N was obtained by PCR using a downstream vector primer and a primer containing the SP6 polymerase binding site and an 18-nucleotide stretch encoding residues 39-44 of Tim54 (5'-GGA TTA GGT GAC ACT ATA GAA ATG ATC TTT TGG TCT GTG-3').

Import of Preproteins Into Isolated Mitochondria

The yeast strains used in this study are shown in Table 1. Mitochondria were isolated from yeast cells grown in YPG media (1% yeast extract, 2% bacto-peptone, and 3% glycerol) according to Daum et al. (1982) and Hartl et al. (1987). Radiolabeled preproteins were obtained by in vitro transcription and translation reactions using rabbit reticulocyte lysate (Amersham, Arlington Heights, IL) in the presence of [³⁵S]methionine/cysteine (Söllner et al., 1991).

Mitochondrial in vitro import reactions were performed in BSA-containing buffer (3% [wt/vol] fatty acid-free BSA, 80 mM KCl, 5 mM MgCl₂, 10 mM MOPS/KOH, pH 7.2) in the presence of 2 mM ATP and 2 mM NADH. To dissipate the membrane potential, 8 µM antimycin A, 20 µM oligomycin, and 1 µM valinomycin (Sigma, St. Louis, MO) were added to the import reaction. Reticulocyte lysate containing radiolabeled preproteins (2.5-10% [vol/vol] of import reaction) was incubated with mitochondria (25-50 µg protein) at 25°C for varying times. Valinomycin (1 µM) was added to stop import, and samples were subsequently treated with or without proteinase K (50 µg/ml) on ice for 15 min. The protease was inactivated by the addition of 1 mM PMSF, and samples were incubated for a further 10 min at 4°C.

For trypsin treatment of accessible Tom receptor domains, mitochondrial samples in SEM buffer (250 mM sucrose, 1 mM EDTA, 10 mM MOPS/KOH, pH 7.2) were incubated with trypsin (20 µg/ml) for 20 min on ice. Trypsin was inactivated on the addition of a 30-fold excess of soybean trypsin inhibitor (Type II-S, Sigma) and samples were incubated for an additional 10 min on ice before further manipulations. For control samples, a 30-fold excess of soybean trypsin inhibitor was added to mitochondria before trypsin addition. Preproteins were imported for 15 min at 25°C before proteinase K digestion.

For import of preproteins into ssc1-3 mitochondria (Gambill et al., 1993), a 15 min incubation at 37°C was performed with both wild-type and ssc1-3 mitochondria before import studies were performed at 25°C.

Swelling of mitochondrial samples was prepared by resuspending the mitochondrial pellets in EM buffer (1 mM EDTA, 10 mM MOPS/KOH, pH 7.2) and incubating the samples on ice for 15 min. ATP was depleted from mitochondrial samples and reticulocyte lysates according to Glick (1995). Preproteins were imported for 15 min at 25°C before proteinase K digestion.

After treatments, mitochondrial pellets were lysed in the appropriate detergent-containing buffer and applied to SDS or blue native polyacrylamide gels.

Accumulation of b₂(167)-dihydrofolate Reductase Across Mitochondrial Membranes

Mitochondria (50 µg) were incubated with 1 µg purified b₂(167)-dihydrofolate reductase (DHFR) (Dekker et al., 1997) for 15 min at 25°C in the presence of 2 µM methotrexate. After accumulation, mitochondria were reisolated, washed with SEM buffer containing 2 µM methotrexate, and finally resuspended in BSA-containing buffer containing 2 µM methotrexate before use in further import assays.

Blue Native Gel Electrophoresis

Blue native PAGE was performed essentially as described previously (Schägger and von Jagow, 1991; Schägger et al., 1994; Dekker et al., 1997). Briefly, mitochondrial pellets (25-100 µg protein) were lysed in 50 µl ice-cold digitonin buffer (1% [wt/vol] digitonin, 20 mM Tris-HCl, pH 7.4, 0.1 mM EDTA, 50 mM NaCl, 10% [vol/vol] glycerol, 1 mM PMSF) (Blom et al., 1995). After a clarifying spin, 5 µl of sample buffer (5% [wt/vol] Coomassie brilliant blue G-250, 100 mM Bis-Tris, pH 7.0, 500 mM 6-aminocaproic acid) were added, and the samples were electrophoresed at 4°C through a 6-16% polyacrylamide gradient gel.

For immunoblotting, the native gel was soaked in blot buffer (20 mM Tris-base, 150 mM glycine, 20% [vol/vol] methanol, 0.08% [wt/vol] SDS) before transfer onto PVDF membranes (Millipore, Bedford, MA) using the semidry blotting technique (Harlow and Lane, 1988). Immunodecoration was performed according to standard procedures (Harlow and Lane, 1988), and detection was achieved using the ECL method (Amersham). For detection of radiolabeled proteins, the dried gel or PVDF membrane was exposed to phosphorimage storage cassettes before phosphorimage analysis (Molecular Dynamics, Sunnyvale, CA).

Miscellaneous

Sequence alignments were generated with MegAlign (DNA Star Inc., Madison, WI) using the Clustal method and the PAM250 weight table.

SDS-PAGE of larger proteins (e.g., Tim54 and Tim22) was performed according to Laemmli (1970), and urea SDS-PAGE (Ito et al., 1980) was used for the analysis of the small Tim proteins.

	RESULTS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERNCES

The Preproteins of Tim22 and Tim54 Require Different Surface Receptors for Import

The preproteins of Tim22 and Tim54 were synthesized in vitro in rabbit reticulocyte lysate in the presence of [³⁵S]methionine/cysteine and incubated with isolated yeast wild-type mitochondria. In the presence of a membrane potential across the inner membrane, the preproteins were transported to a protease-protected location (Figure 1A, lanes 1-3 and 5-7). On dissipation of the , import was blocked (Figure 1A, lanes 4 and 8).

View larger version (51K): [in this window] [in a new window]   Figure 1.   Biogenesis of Tim22 and Tim54. (A) Import kinetics of Tim22 and Tim54. 35S-labeled Tim22 and Tim54 preproteins were imported into mitochondria for different times in the presence (lanes 1-3, 5-7) or absence (lanes 4 and 8) of a membrane potential () as described in MATERIALS AND METHODS. After import, mitochondria were treated with 50 µg/ml proteinase K and subjected to SDS-PAGE. (B) Assembly of Tim22 and Tim54 into the carrier translocase. Mitochondria were solubilized in digitonin-containing buffer and subjected to blue native gel electrophoresis. The gel was blotted, and lanes were immunodecorated with antibodies specific for Tom40 (lane 1), Tim23 (lane 2), or Tim22 (lane 3). For lanes 4-11, Tim22 and Tim54 preproteins were imported into mitochondria as in A, and mitochondria were electrophoresed on blue native PAGE as above. (C) The import of Tim54 and Tim22 requires surface receptors. Mitochondria were pretreated with 20 µg/ml trypsin for 20 min, before inactivation with a 30-fold excess of trypsin inhibitor (lane 2). For control samples (lane 1), trypsin-inhibitor was added to mitochondria before the addition of trypsin. 35S-labeled Tim22 and Tim54 preproteins were subjected to mitochondrial import as well as 35S-labeled porin, AAC, and Su9-DHFR preproteins, which served as controls. Samples were treated with proteinase K before SDS-PAGE analysis. (D) The import of Tim22 and Tim54 show differential requirements for ATP. 35S-labeled Tim22, Tim54, AAC, and Su9-DHFR in reticulocyte lysates were depleted of ATP. Preproteins were imported into mitochondria in the presence (lane 1) or absence of added ATP. In lane 2, mitochondria were additionally blocked in the export of ATP from the matrix according to Glick (1995). Samples were treated with proteinase K before SDS-PAGE analysis. Radiolabeled proteins were detected by storage phosphorimage cassette technology.

To determine whether the proteins were correctly imported and assembled, we used blue native electrophoresis of digitonin-lysed mitochondria, which allows a separation of the mitochondrial translocase complexes (Dekker et al., 1996-1998): the large Tom complex, termed the GIP complex (Figure 1B, lane 1); the Tim core complex containing Tim23 and Tim17 (Figure 1B, lane 2); and a ~300-kDa complex containing Tim22, termed the carrier translocase (Figure 1B, lane 3). We found that both imported Tim22 and Tim54 were efficiently assembled into the 300-kDa translocase complex in a -dependent manner (Figure 1B, lanes 4-6 and 8-10).

By a pretreatment of the mitochondria with trypsin, the cytosolic domains of the import receptors Tom20, Tom22, and Tom70 are removed (Alconada et al., 1995; Dietmeier et al., 1997). The import of both Tim22 and Tim54 into trypsin-treated mitochondria was inhibited, indicating a dependence on one or more of these surface receptors (Figure 1C, lane 2). Such pretreatment also inhibited the import of the outer membrane protein porin and a matrix-targeted fusion protein between the presequence of F_o-ATPase subunit 9 and the entire dihydrofolate reductase (Su9-DHFR), which mainly uses Tom20 (Hines et al., 1990; Moczko et al., 1994; Pfanner et al., 1997). The import of AAC, which mainly uses Tom70 as its receptor (Hines et al., 1990; Söllner et al., 1990), was also inhibited (Figure 1C). It has been shown previously that there is a differential dependence on cytosolic ATP and cofactors for the targeting of preproteins to either Tom20 or Tom70 (Hachiya et al., 1995; Komiya et al., 1996, 1997). We asked how the depletion of cytosolic ATP affected the import of Tim22 and Tim54 and found a strong difference between both preproteins. Although the import of Tim54 was inhibited by the ATP depletion, the import of Tim22 was unchanged (Figure 1D, lane 2). As a control, we show that depletion of cytosolic ATP also inhibited the import of the preprotein AAC but not Su9-DHFR (Figure 1D) (Wachter et al., 1994).

We thus examined the possibility that Tim22 and Tim54 interacted with different receptors by using mitochondria isolated from yeast strains lacking TOM20 or TOM70 genes, respectively. Because a lack of Tom20 causes a reduction in the mitochondrial levels of Tom22 and thus indirectly a reduction of the mitochondrial membrane potential (Lithgow et al., 1994; Gärtner et al., 1995b; Hönlinger et al., 1995b), we used a tom20 strain where TOM22 was put on a high-copy number plasmid to restore the mitochondrial levels of Tom22 and the membrane potential (Hönlinger et al., 1995b). Import of the preprotein of Tim22 was strongly inhibited in tom20 mitochondria but practically unchanged in tom70 mitochondria in comparison to wild-type mitochondria (Figure 2, left panel). In contrast, the import of Tim54 was strongly inhibited in tom70 mitochondria, but only mildly affected in tom20 mitochondria (Figure 2, middle panel). Furthermore, the presequence-containing preprotein Su9-DHFR displayed import characteristics similar to those of Tim22 in its receptor requirements (Figure 2, right panel). In the absence of a membrane potential across the inner membrane, import of the preproteins was inhibited in all cases (Figure 2, lanes 5, 10, and 15), confirming the specificity of the import processes into the mutant mitochondria.

View larger version (35K): [in this window] [in a new window]   Figure 2.   Tim22 and Tim54 require different outer membrane receptors for their import into mitochondria. In vitro synthesized 35S-labeled Tim22 (left panel), Tim54 (middle panel), and Su9-DHFR (right panel) were imported into mitochondria isolated from wild-type (WT), tom70, tom20, or tom5 yeast cells for the indicated times in the presence (lanes 1-4, 6-9, and 11-14) or absence (lanes 5, 10, and 15) of a membrane potential (). Samples were treated with proteinase K before SDS-PAGE analysis. Radiolabeled proteins were detected by storage phosphorimage cassette technology and quantitated using ImageQuant software (Molecular Dynamics). The import observed in wild-type mitochondria after 20 min was set at 100% (control).

In addition, we used mitochondria from a yeast strain lacking the small subunit Tom5 of the GIP complex. Tom5 is resistant to a treatment with trypsin and functions after the surface receptors at the entry site of the import pore where the presequence and carrier routes converge (Dietmeier et al., 1997). The import of both Tim22 and Tim54 along with Su9-DHFR were inhibited in tom5 mitochondria (Figure 2, bottom panels). We conclude that the preprotein of Tim54 is directed into mitochondria preferentially by Tom70 in an ATP-dependent reaction, whereas the preprotein of Tim22, like the presequence-containing preprotein Su9-DHFR, uses Tom20 as receptor in a manner independent of cytosolic ATP. Both import pathways join at the entry site of the GIP that includes Tom5.

Different Tim Pathways for the Preproteins of Tim22 and Tim54

Koehler et al. (1998a) and Sirrenberg et al. (1998) showed that mutations in Tim components of the carrier pathway, including Tim10, caused a strong reduction in the mitochondrial level of Tim22, suggesting that the preprotein of Tim22 itself was imported via the carrier Tim pathway, as shown previously for the homologous proteins Tim23 and Tim17 (Dekker et al., 1997; Kerscher et al., 1997; Káldi et al., 1998). We used two assays to determine which Tim pathway was used by the preprotein of Tim54 in comparison with the import of Tim22.

Accumulation of Chemical Amounts of a Presequence-Containing Preprotein in Translocation Contact Sites A matrix-targeted preprotein, consisting of a portion of precytochrome b₂ and the entire dihydrofolate reductase [b₂(167)-DHFR], can be prepared as a soluble species in large amounts. After stabilization of the DHFR moiety with methotrexate, the preprotein can be accumulated in the mitochondrial import sites, spanning both the Tom machinery and the Tim23-Tim17 machinery (Dekker et al., 1997). Thereby the subsequent import of preproteins using the Tim23-Tim17 pathway is impaired in mitochondria with accumulated b₂(167)-DHFR. The import of carrier proteins is only slightly affected because the Tom complexes are approximately four times more abundant than the Tim23-Tim17 complexes, and thus most Tom complexes are not occupied by the b₂(167)-DHFR preprotein (Dekker et al., 1997). We accumulated methotrexate-bound b₂(167)-DHFR in mitochondria and tested the import kinetics of different preproteins. Although the import of Tim22 was only slightly inhibited (Figure 3A), a significant reduction in import was observed for the preprotein of Tim54 (Figure 3B). For comparison, the import of AAC was only slightly affected (Figure 3C), but the import of the Rieske Fe/S protein, a typical presequence-containing preprotein, was inhibited (Figure 3D). Furthermore, the analysis of inner-membrane insertion and assembly of the imported preproteins by blue native electrophoresis revealed that Tim54 assembly was reduced in mitochondria containing accumulated preprotein (Figure 3E).

View larger version (42K): [in this window] [in a new window]   Figure 3.   The preproteins of Tim22 and Tim54 use different Tim pathways for their import. Mitochondria were incubated in the presence or absence of b2(167)-DHFR and methotrexate for 15 min at 25°C. Mitochondria were reisolated, washed, and resuspended in import buffer containing methotrexate before the addition of 35S-labeled (A) Tim22, (B) Tim54, (C) the ADP/ATP carrier (AAC), or (D) the Fe/S protein (Fe/Sp). Import proceeded for the indicated times, and mitochondria were subsequently treated with 50 µg/ml proteinase K. Where indicated, the membrane potential () was dissipated before the addition of preprotein. Mitochondrial proteins were separated by SDS-PAGE and radiolabeled proteins were detected by storage phosphorimage cassette technology. Processing of the presequence of the Fe/S protein by matrix-located proteases generates intermediate (i) and mature (m) forms as indicated. The amount of imported preprotein was quantitated using ImageQuant software. The import in the absence of b2(167)-DHFR after the longest import time was set to 100% for each preprotein. (E) Tim22, Tim54, and AAC preproteins were imported into mitochondria for 30 min in the presence or absence of accumulated b2(167)-DHFR and subjected to blue native PAGE. Assembled preprotein was quantitated using ImageQuant software where 100% was set to preprotein assembled in the absence of b2(167)-DHFR.

A Point Mutation in TIM23 Mitochondria from the yeast mutant tim23-2 carry an amino acid substitution in Tim23 that causes a labilization of the Tim23-Tim17 complex and thus a reduction in preproteins imported via Tim23-Tim17 (Bömer et al., 1997a; Dekker et al., 1997). The import of Tim22 into tim23-2 mitochondria occurred with wild-type rates, like the import of the ADP/ATP carrier (Figure 4A, first and third panels). The import of Tim54 into tim23-2 mitochondria was partly reduced, comparable to the import of the Fe/S protein (Figure 4A, compare second and fourth panels, lanes 5-7 with lanes 1-3; Figure 4B, compare columns 2 and 4 [showing the degree of inhibition]).

View larger version (43K): [in this window] [in a new window]   Figure 4.   Dependence of Tim54 import on Tim23 and matrix Hsp70. (A) The import of the Fe/S protein and Tim54 is impaired in tim23-2 mitochondria. 35S-labeled preproteins of Tim22, Tim54, the ADP/ATP carrier (AAC), or the Fe/S protein (Fe/Sp) were imported into mitochondria isolated from wild-type (WT) cells or tim23-2 cells in the presence (lanes 1-3, 5-7) or absence (lanes 4 and 8) of a membrane potential () for the indicated times. After import, samples were treated with 50 µg/ml proteinase K and subjected to SDS-PAGE. (B) The inhibition of preprotein import in tim23-2 mitochondria observed after 15 min import was quantified in comparison to the import yield into wild-type mitochondria (control). (C) Inactivation of matrix Hsp70 has a partial effect on the import of Tim54 but no effect on Tim22. 35S-labeled preproteins corresponding to Tim22, Tim54, the -subunit of the F1-ATPase (F1), or the Fe/S protein (Fe/Sp) were imported into wild-type mitochondria and mitochondria containing a temperature-sensitive mutation in SSC1, encoding the matrix-located Hsp70 (ssc1-3). Processing of F1 generates the mature (m) product as shown. After import, samples were treated with 50 µg/ml proteinase K and subjected to SDS-PAGE. The amount of protein imported into wild-type mitochondria after 15 min was set to 100% (control).

View larger version (26K): [in this window] [in a new window]   Figure 5.   Different portions of Tim54 are needed for receptor interaction and membrane translocation. (A) Generation of a construct lacking the first 38 amino acids of Tim54 (Tim54N) removes a number of basic residues. Positively and negatively charged residues are indicated. Tim54N initiates from the internal methionine shown at position 39. (B) Tim54N is not imported into mitochondria. 35S-labeled preproteins corresponding to Tim54 (lanes 1-4) and Tim54N (lanes 5-8) were incubated with mitochondria for the indicated times in the presence (lanes 1-3, 5-7) or absence (lanes 4 and 8) of a membrane potential (). After import, samples were halved and treated with (bottom panel) or without (top panel) proteinase K (Prot. K). (C) Binding of Tim54N to mitochondria is inhibited by a lack of Tom70. 35S-labeled Tim54 and Tim54N preproteins were arrested on the outer membrane by depleting ATP from mitochondria and reticulocyte lysates. Samples were analyzed by SDS-PAGE, and radiolabeled proteins were quantified using ImageQuant software. Binding to isolated mitochondria from wild-type cells (WT; control) was compared with binding to mitochondria lacking Tom20 (tom20) or Tom70 (tom70).

A Role for Tom5 in Targeting of the Small Tims to Mitochondria

Tim9, Tim10, and Tim12 are the three currently known small Tim proteins that are homologous to each other. A search in the yeast genome revealed the presence of two additional small Tim proteins, termed Tim8 and Tim13, with significant homology to the other three Tims, including a complete conservation of four cysteine residues (Figure 6A). The preproteins of the small Tims were synthesized in reticulocyte lysates and labeled with [³⁵S]methionine/cysteine. Like the known small Tims, Tim8 and Tim13 were transported to a protease-protected location in mitochondria (Figure 6B, lane 1). After swelling of the mitochondria that led to an opening of the intermembrane space with an efficiency of ~80-90% (Bömer et al., 1997a,b), each small Tim became accessible to protease (Figure 6B, lane 2), demonstrating that Tim8 and Tim13 also were located in the intermembrane space. As control, immunodecoration showed that endogenous Tim10 along with the intermembrane space protein cytochrome b₂ were accessible to protease after swelling but not the matrix-located protein Mge1 (Figure 6B, Immunodecoration).

View larger version (63K): [in this window] [in a new window]   Figure 6.   A family of small Tim proteins with a preference for import via Tom5. (A) Identification of two new small Tim homologues. Sequence alignment of the five small Tim proteins. Amino acid identities are shown in white lettering against black; conserved residues are shown in black lettering against gray. The putative Zn-finger motif cysteines are indicated by an asterisk above. (B) The small Tim proteins are imported into the intermembrane space. 35S-labeled Tim13, Tim12, Tim10, Tim9, and Tim8 preproteins were imported into mitochondria for 10 min at 25°C. After import, mitochondria were isolated and treated with proteinase K (lane 1) or first swollen before proteinase K treatment (lane 2). As controls for swelling and proteinase K treatment, mitochondria were immunodecorated with antibodies against the intermembrane space proteins Tim10 and cytochrome b2 (Cyt b2) and the matrix protein Mge1. (C) Mitochondrial import of the small Tim proteins can occur in the absence of trypsin-accessible receptor domains. Before import of 35S-labeled Tim9, Tim10, and Tim13, mitochondria were pretreated with active (lanes 2, 4, 6) or inactive trypsin (lanes 1, 3, 5; control). All import reactions were treated with proteinase K before SDS-PAGE and quantification. (D) The import of the small Tim proteins preferentially depends on Tom5. 35S-labeled Tim9 and Tim13 were imported into wild-type (WT) mitochondria or mitochondria lacking Tom70 (tom70), Tom20 (tom20), or Tom5 (tom5) for the indicated time points. Samples were treated with proteinase K and analyzed by SDS-PAGE and digital autoradiography.

We then investigated the targeting principles of the small Tims. No obvious mitochondrial targeting signals are observed in any of the small Tim sequences (Figure 6A), and our unpublished results along with those of Koehler et al. (1998b) showed that the small Tim proteins do not require a membrane potential for their insertion into the intermembrane space. Surprisingly, a pretreatment of the mitochondria with trypsin did not inhibit the import of the small Tims, as shown here with the preproteins of Tim9, Tim10, and Tim13 (Figure 6C, columns 2, 4, and 6), indicating that they did not strictly require mitochondrial surface receptors. Indeed, import of the small Tims was not affected by a deletion of TOM70 (Figure 6D, tom70) and was only slightly impaired, in the case of Tim9, by a deletion of TOM20 (Figure 6D, tom20). We wondered whether the small Tim proteins showed a requirement for Tom5 as receptor because Tom5 is resistant to trypsin treatment and can suffice as a receptor under bypass import conditions (Dietmeier et al., 1997). Indeed, the import of the small Tims was strongly inhibited in tom5 mitochondria (Figure 6D, tom5). Previous work showed that the tom5 mitochondria are selectively deficient in Tom5, whereas the other Tom proteins are present in normal amounts and the GIP complex is not altered (Dietmeier et al., 1997; Dekker et al., 1998). Although the import of preproteins in tom5 mitochondria is generally reduced compared with wild-type mitochondria (Dietmeier et al., 1997), the small Tim proteins showed an even stronger reliance on Tom5 for their import compared with Tim22 and Tim54 (Figure 2). We conclude that Tom5, but not the trypsin-accessible surface receptors, plays an important role in the targeting of the small Tims into mitochondria.

	DISCUSSION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERNCES

The biogenesis of the Tim proteins of the carrier import route neither follows one of the known major import pathways for mitochondrial preproteins (Figure 7A, routes I and II) nor fits into a common new mechanism. Three different targeting principles seem to be necessary to import the Tim components of the carrier route (Figure 7B).

View larger version (39K): [in this window] [in a new window]   Figure 7.   Multiple targeting routes of preproteins into mitochondria. (A) Established pathways for presequence-containing preproteins and carrier preproteins. Presequence-containing preproteins (I) follow an import route that preferentially involves binding to Tom20, whereas carrier preproteins (II) preferentially bind to Tom70. The pathways converge at the general import pore (GIP) consisting of, among other components, Tom5 and Tom40. The pathways diverge after translocation across the outer membrane and use different Tim complexes: the Tim23-Tim17 core complex along with mt-Hsp70 and Tim44 for presequence-carrying preproteins and the carrier translocase for carrier preproteins. The requirement of mtHsp70 for the import of preproteins using pathway I, but sorted to the inner membrane, seems to be sequence dependent. (B) Three distinct routes for the biogenesis of Tim proteins of the carrier pathway. Shown are the main import routes that are used by the Tim proteins of the carrier pathway. The import of Tim54 and Tim22 uses different features of the presequence-carrying preprotein and carrier preprotein pathways to reach the same destination. The small Tims seem to preferentially require Tom5, but not the surface receptors, for their import.

The preprotein of Tim22 is the first preprotein found that preferentially uses the receptor Tom20 for targeting to the outer membrane but follows the Tim route for carrier proteins (Figure 7B, route III). Tim22 is a quite hydrophobic protein and therefore would have been a typical candidate for binding to Tom70 like the carrier preproteins, but import signals in Tim22 that are not yet defined direct the preprotein to Tom20, which usually functions as a receptor for presequence-containing preproteins.

In contrast, Tim54 carries an amino-terminal noncleaved translocation sequence that is positively charged like mitochondrial presequences, yet Tim54 preferentially depends on Tom70 for its targeting to the outer membrane (Figure 7B, route IV). After translocation through the GIP, Tim54 then follows the typical route for presequence-containing preproteins until it reaches the Tim23-Tim17 core complex. The amino-terminal positively charged sequence of Tim54 is required for translocation of the preprotein into the mitochondria, whereas interaction with the outer membrane can occur without this sequence. This indicates that the remainder of Tim54, which includes two predicted hydrophobic segments (Kerscher et al., 1997), contains a signal for its interaction with Tom70. Although carrying an abundance of positive residues, the amino-terminal sequence of Tim54 is not predicted to form an amphipathic -helix because of the presence of several helix-breaking prolyl residues, distinguishing this sequence from typical mitochondrial presequences (Roise et al., 1986; Von Heijne, 1986) and providing an explanation for the only weak dependence on Tom20. Tim54 branches from the main import route at the level of Tim23 before a strict requirement for matrix Hsp70 becomes crucial. It has been observed that a hydrophobic signal anchor following the positively charged amino-terminal region of a preprotein minimizes its requirement for mtHsp70. This is apparently due to the sorting/membrane insertion activity of the hydrophobic segment at an early stage of translocation of an unfolded preprotein (Glick et al., 1993; Voos et al., 1993; Stuart et al., 1994; Gärtner et al., 1995a, 1995b). In agreement with this model, the first predicted hydrophobic segment of Tim54 is located immediately after the positively charged sequence (Kerscher et al., 1997).

In an elegant series of experiments, Mihara and colleagues (Hachiya et al., 1995; Komiya et al., 1996, 1997) demonstrated that cytosolic cofactors from rabbit reticulocyte lysate are important determinants for the selection of import receptors by preproteins. By using purified preproteins and cytosolic chaperones, they showed that preproteins bound to the mitochondrial import stimulation factor are imported via Tom70 in an ATP-dependent reaction, whereas preproteins interacting with cytosolic Hsp70 preferentially use Tom20 in an ATP-independent manner. In agreement with these observations we found that the import of Tim22 via Tom20 did not require cytosolic ATP, whereas the import of Tim54 via Tom70 was ATP-dependent. Because we used complete rabbit reticulocyte lysate, the full set of cytosolic chaperones was present during the import reaction, suggesting that the preproteins of Tim22 and Tim54 were bound to different chaperones before their delivery to the mitochondria. Some preproteins bound to Tom70 seem to be transferred to Tom20 before their insertion into the GIP, i.e., they require both Tom70 and Tom20 for import (Keil and Pfanner, 1993; Keil et al., 1993; Hachiya et al., 1995; Hönlinger et al., 1995a; Komiya et al., 1997), whereas other preproteins can be directly transferred from Tom70 to the GIP complex (Söllner et al., 1990; Steger et al., 1990). The preprotein of Tim54 mainly behaves like the latter preproteins. Tim54 bound to Tom70 does not strictly need to be transferred to Tom20 but can be directly transferred to the GIP complex in the absence of Tom20.

A third and quite short import pathway is followed by the small Tim proteins (Figure 7B, route V). The small Tims are directly translocated into the intermembrane space without an insertion into the inner membrane because their import does not require an inner membrane potential. Other intermembrane space proteins that only use the Tom machinery are the cytochrome c heme lyase and the cytochrome c₁ heme lyase. These preproteins strongly require Tom20 for their transfer to the GIP complex (Lill et al., 1992; Steiner et al., 1995). For the import of the small Tims, however, the trypsin-accessible surface domains of import receptors were dispensable, including Tom20, Tom22, and Tom70. Here, the trypsin-resistant Tom5 that typically functions as the link between the receptor and the general import pore (Dietmeier et al., 1997) plays the crucial role. Tom5 seems to represent the first Tom protein that interacts with the majority of preproteins of the small Tims. Besides the import of apocytochrome c (Stuart et al., 1990a,b), the import of the small Tims is therefore one of the simplest mitochondrial membrane translocation mechanisms known to date.

In addition to the various targeting pathways, this study led to two additional pieces of information. 1) Two new homologues of the small Tims, termed Tim13 and Tim8, were identified and found to be located in the intermembrane space. The four cysteines that were suggested to be of functional importance for the small Tims by formation of Zn-finger-like motifs are fully conserved in both Tim8 and Tim13. These small Tims were also identified independently as mitochondrial intermembrane space proteins interacting with Tim9 and Tim10 (Koehler et al., 1999). 2) Blue native electrophoresis provides a simple and efficient method to assess the correct assembly of in vitro imported Tim22 and Tim54 into a ~300-kDa complex. This complex also contains peripherally associated Tim12 (Koehler et al., 1998a,b; Sirrenberg et al., 1998; our unpublished results) and is termed the carrier translocase.

In summary, we conclude that multiple mechanisms exist for targeting and membrane translocation of mitochondrial preproteins. Depending on the preprotein, distinct pieces of the known major import routes are combined to yield novel pathways. This includes crossing over of pathways at the level of the GIP complex of the outer membrane. For special preproteins like the small Tims, Tom5, which typically functions at the second or third stage of import, can become the first level import component and may thus have receptor-like functions.