INTRODUCTION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

The plasma membrane of polarized epithelial cells is divided into two functionally and biochemically distinct domains, the apical and basolateral plasma membranes (Simons and Fuller, 1985). Junctional complexes such as tight junctions, adherens junctions, and desmosomes play crucial roles in the structure and function of epithelial cells. The tight junction forms a continuous belt at the boundary between the apical and lateral plasma membrane domains and selectively regulates the passage of molecules across the paracellular pathway (gate function) and passively separates molecules into the apical and basolateral plasma membrane domains (fence function) (Farquhar and Palade, 1963). The adherens junction, localized below the tight junction, consists of cell adhesion and signaling molecules and may regulate the formation of other junctional complexes (Yap et al., 1997). Desmosomes are button-like adhesion points that provide resilience and tensile strength to the epithelial monolayer (Garrod et al., 1996).

The calcium-dependent cell-cell adhesion molecule E-cadherin has been implicated in the regulation of the epithelial phenotype. E-cadherin mediates cell-cell contact between epithelial cells by homophilic interaction (Takeichi, 1990). The cytoplasmic tail of E-cadherin associates with -, -, and -catenins (Ozawa et al., 1989) and p120^ctn (Shibamoto et al., 1995). -Catenin links the E-cadherin complex to the actin cytoskeleton, which is crucial for the cell adhesion function of E-cadherin (Knudsen et al., 1995; Rimm et al., 1995). Earlier studies have shown that disruption of E-cadherin-mediated adhesive cell-cell contacts between epithelial cells prevents the assembly of tight junctions and consequently abolishes epithelial polarity (Behrens et al., 1985; Gonzalez-Mariscal et al., 1985; Gumbiner and Simons, 1986).

Loss of the polarized phenotype of carcinoma cells has been attributed to the loss of E-cadherin function. Analysis of many tumors derived from epithelial tissues suggests that loss of E-cadherin function seems to be a key event and correlates with tumor cell invasiveness (Stetler-Stevenson et al., 1993; Birchmeier and Behrens, 1994). Because reexpression of E-cadherin suppresses tumor invasiveness in vitro (Frixen et al., 1991), E-cadherin has been termed an invasion suppressor (Vleminckx et al., 1991). In spite of these circumstantial evidences the mechanism by which E-cadherin-mediated cell adhesion regulates epithelial polarity or invasion suppression is poorly understood.

The Na,K-ATPase consisting of two noncovalently linked - and -subunits catalyzes an ATP-dependent transport of three sodium ions out and two potassium ions into the cell per pump cycle, thereby generating a transmembrane sodium gradient. The ₁-subunit (~112 kDa; Shull et al., 1985) contains the catalytic and ligand binding sites of the enzyme. Of the four -isoforms described in mammals (Shamraj and Lingrel, 1994; Blanco et al., 1999; Woo et al., 1999), the predominant -isoform in kidney is ₁ (Mercer, 1993).

The ₁-subunit (~55 kDa; Shull et al., 1986) is a glycosylated protein and its role in Na,K-ATPase enzyme function remains somewhat obscure. It may modulate the transport of Na⁺ and K⁺ across the membrane (Eakle et al., 1994) and facilitate the insertion of the -complex into the cell membrane (Noguchi et al., 1990; Geering, 1991; Geering et al., 1996). Of the three isoforms described (Lingrel et al., 1994) the predominant isoform in kidney is ₁ (Mercer, 1993).

Na,K-ATPase is a central regulator of kidney functions (Laski and Kurtzman, 1996). Therefore, we studied its levels and activity in renal cell carcinoma and found that the ₁-subunit is markedly reduced in clear-cell renal cell carcinoma, an invasive and the most prevalent form of kidney cancer (Rajasekaran et al., 1999). Subsequently, we found that Moloney sarcoma virus-transformed MDCK (MSV-MDCK) cells have highly reduced Na,K-ATPase ₁-subunit levels. Previous studies correlated the invasive phenotype of MSV-MDCK cells to reduced expression of E-cadherin (Behrens et al., 1989). We demonstrate that E-cadherin's cell-cell adhesion function is not sufficient to either reestablish epithelial polarity or suppress invasiveness and cell motility in these cells. In contrast, ectopic expression of both E-cadherin and Na,K-ATPase ₁-subunit in MSV-MDCK cells induced junctional complexes, reestablished epithelial polarity, and suppressed invasiveness and motility, indicating that Na,K-ATPase ₁-subunit function is necessary for induction of a polarized phenotype and invasion suppression of MSV-MDCK cells.

	MATERIALS AND METHODS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Plasmid Constructs

Canine Na,K-ATPase ₁-subunit in pKS⁺ was a gift from Dr. Robert Farley (University of Southern California, Los Angeles, CA). The polymerase chain reaction-amplified cDNA was subcloned into pCDNA3 (Invitrogen, Carlsbad, CA) and the sequence was confirmed by DNA sequencing. Canine Na,K-ATPase ₁-subunit in plasmid bluescript was kindly provided by Dr. Askari Amir (Medical College of Ohio, Toledo, OH) and subcloned into pCDNA3. Canine E-cadherin cDNA in the eukaryotic expression vector pR2 was a gift from Dr. Lisa McConlogue (Athena Neurosciences, South San Francisco, CA). The pCB7 expression vector containing a hygromycin resistance gene was provided by Dr. Michael Roth (University of Texas Southwestern Medical Center, Dallas, TX).

Cell Lines and DNA Transfection

MDCK_wt and MSV-transformed MDCK cells (DoCl1, designated here as MSV-MDCK) were obtained from American Type Culture Collection (Rockville, MD) and grown as described previously (Rajasekaran et al., 1996). The Na,K-ATPase ₁-subunit cDNA was transfected into MSV-MDCK cells by using the calcium phosphate method and stable clones were selected 2 wk after addition of 450 µg/ml G418 (Geneticin; Life Technologies, Gaithersburg, MD). MSV-MDCK clones expressing the ₁-subunit (MSV-NaK-cl 1 and MSV-NaK-cl 2) were identified by immunofluorescence and immunoblot analysis. MSV-MDCK vector cells transfected with pCDNA3 vector and selected in parallel with the other clones served as controls. MSV-MDCK cells expressing E-cadherin (MSV-Cad) were described previously (Rajasekaran et al., 1996). Cell lines expressing E-cadherin and Na,K-ATPase ₁-subunit (MSV-Cad-NaK) were obtained by transfecting MSV-Cad cells with 10 µg of Na,K-ATPase ₁-subunit in pCDNA3 and 2 µg of pCB7. Two stable clones (MSV-Cad-NaK-cl 1, MSV-Cad-NaK-cl 2) were selected after treatment with 250 µg/ml hygromycin; expression of E-cadherin and the -subunit was confirmed by immunoblot and immunofluorescence analysis. MSV-Cad vector cells are MSV-Cad cells transfected with pCDNA3 and pCB7 and were selected in parallel with MSV-Cad-NaK cells. Cells expressing E-cadherin and Na,K-ATPase ₁-subunit (MSV-Cad-NaK) were obtained by transfecting MSV-Cad cells with 10 µg of canine Na,K-ATPase ₁-subunit in pCDNA3 and 2 µg pCB7. The clones were selected with hygromycin as described above. All selected clones were maintained in culture medium containing the appropriate selection antibiotics. Revertents of MSV-MDCK cells expressing E-cadherin and Na,K-ATPase -subunit (MSV-Cad-NaK rev) were obtained by maintaining MSV-Cad-NaK-cl 1 in the absence of G418 and hygromycin for at least 5 mo.

Antibodies

Mouse monoclonal antibodies (mAbs) raised against Na,K-ATPase - (M7-PB-E9) and -subunit (M17-P5-F11) recognize epitopes that are common in human, sheep, and dog and have been characterized and described previously (Abbott and Ball, 1993; Sun and Ball, 1994). Mouse mAb against canine E-cadherin was a gift from Dr. Barry Gumbiner (Memorial Sloan Kettering Institute, New York, NY). Rabbit polyclonal zonula occludens-1 (ZO-1) antibody was purchased from Zymed Laboratories (South San Francisco, CA), mouse anti--catenin from Transduction Laboratories (Lexington, KY) and fluorescein isothiocyanate (FITC)-conjugated phalloidin from Sigma Chemical (St. Louis, MO). FITC- and Texas Red-labeled, affinity-purified secondary antibodies were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA) and horseradish peroxidase (HRP)-anti-mouse antibody and HRP-protein A from Transduction Laboratories.

Immunofluorescence and Laser Scanning Confocal Microscopy

Confluent monolayers grown on poly(D-lysine) (50 µg/ml) and laminin (50 µg/ml) (Collaborative Biomedical Products, Bedford, MA)-coated glass coverslips were fixed in ice-cold methanol and processed for immunofluorescence as described previously (Rajasekaran et al., 1996). Epifluorescence analysis was performed by using an Olympus AX 70 (Provis) microscope.

To visualize F-actin confluent monolayers were fixed with 2% paraformaldehyde, quenched with 50 mM ammonium chloride, permeabilized with 0.075% saponin (Sigma Chemical), and stained with FITC-conjugated phalloidin. Images were recorded with a 1008 × 1018 cooled charge-coupled device camera (Life Science Resources, Cambridge, United Kingdom) mounted on an Olympus AX 70 (Provis) microscope and analyzed by using Esprit software.

The relative distributions of ZO-1 and E-cadherin were examined by using a Fluoview laser scanning confocal microscope (Olympus America, Melville, NY) as described previously (Rajasekaran et al., 1996). Three-dimensional images were obtained by using the Fluoview image analysis software (version 2.1.39).

Immunoblotting

Confluent monolayers were lysed in lysis buffer (95 mM NaCl, 25 mM Tris pH 7.4, 0.5 mM EDTA, 2% SDS, 1 mM phenylmethylsulfonyl fluoride [PMSF], 5 µg/ml each of antipain, leupeptin, pepstatin), briefly sonicated, and centrifuged at 14,000 rpm in a microfuge for 10 min. Equal amounts of protein (50 or100 µg) were separated by SDS-PAGE (7.5% or 10%) and transferred to nitrocellulose membrane (Schleicher & Schuell, Keene, NH). After blocking with 10% nonfat dry milk/PBS, the blots were incubated for 2 h at room temperature (RT) with primary antibody, washed with PBS/0.3% Tween, and incubated for 1 h at RT with either HRP-conjugated anti-mouse secondary antibody or HRP-conjugated protein A. Bound antibody was detected by enhanced chemiluminescence (NEN Life Science Products, Boston, MA). Densitometric analysis was carried out with an ImageQuant software package (Molecular Dynamics, Sunnyvale, CA).

Cell Surface Biotinylation

The cell surface of subconfluent monolayers was labeled on ice with 0.5 µg/ml membrane-impermeable EZ-Link Sulfo-NHS-Biotin (Pierce, Rockford, IL) in tetraethylammonium (150 mM NaCl, 10 mM triethanolamine pH 9, 1 mM CaCl₂, 1 mM MgCl₂). After quenching (50 mM ammonium chloride in PBS, 0.1 mM CaCl₂, 1 mM MgCl₂) the cells were lysed in 0.5 ml of lysis buffer (150 mM NaCl, 20 mM Tris pH 8, 5 mM EDTA, 1% Triton X-100, 0.1% bovine serum albumin [BSA], 1 mM PMSF, 5 µg/ml each of antipain, leupeptin, pepstatin). Protein (300 µg) of each lysate was used for precipitation (16 h at 4°C) with 30 µl of Ultralink streptavidin beads (Pierce). The precipitates were washed as described previously (Rajasekaran et al., 1996) and immunoblotted (see above).

Electron Microscopy

Confluent monolayers were fixed in 2.5% glutaraldehyde/0.1 M sodium cacodylate buffer, pH 7.4, processed by conventional procedures for electron microscopy, and examined with an electron microsocope (Jeol 1200EXII) at 80 kV.

Antibody Permeability Assay

Confluent cell monolayers on glass coverslips were fixed in 2% paraformaldehyde under nonpermeabilized conditions and labeled with a monoclonal antibody against E-cadherin. The cells were washed and stained with FITC-conjugated anti-mouse secondary antibody and visualized by an Olympus AX 70 (Provis) microscope as described above.

Collagen Invasion

Ice-cold rat tail collagen, type I (~4 mg/ml; Collaborative Biomedical Products) was added to an equal volume of 2× DMEM/20% fetal bovine serum. The pH was adjusted to ~pH 7.4 with 1 N NaOH. In a 12-well tissue culture plate, a bottom layer of 500 µl of collagen/DMEM was polymerized at 37°C. Cells (5000) of a single cell suspension were added to 1 ml of collagen/DMEM, layered on top of the bottom gel, and allowed to polymerize at 37°C. The cells were grown for 10-14 d at 37°C, 5% CO₂. Photographs were taken with an Olympus CK2 inverted phase contrast microscope.

Transwell Motility Assay

Twelve-well PET-membrane cell culture inserts with 8.0-µm pores (Becton Dickinson Labware, Franklin Lakes, NJ) were coated from the bottom with 50 µg/ml rat tail collagen type I. Single-cell suspensions were washed once with DMEM/BSA (5 mg/ml) and 100,000 cells each were plated on the coated filters. After 18-h incubation in DMEM/BSA at 37°C, 5% CO₂ the filters were removed and cells attached to the bottom of the well were counted.

Wound Assay

A uniform cell-free area was created by scratching confluent monolayers with a plastic pipet tip and the wound area was inspected regularly. At each time point four photographs were taken and the distance between the two opposing edges was measured at two points on each photograph. The distance migrated in micrometers was calculated as difference of the scratch width at 0 h and that at the time point indicated.

Triton X-100 Extraction

Confluent monolayers were extracted with 200 µl of extraction buffer [50 mM NaCl, 10 mM piperazine-N,N'-bis(2-ethanesulfonic acid) pH 6.8, 3 mM MgCl₂, 0.5% Triton X-100, 300 mM sucrose, 1 mM PMSF, 100 U/ml DNase] for 10 min at 4°C. The lysates were centrifuged at 4°C for 30 min at 14,000 rpm in a microfuge and the pellet was resuspended in 200 µl of 2× sample buffer. Equal volumes of each pellet and supernatant fraction were subjected to immunoblotting as described above.

Cell Aggregation Assay

Cells were washed briefly in PBS and trypsinized with 0.05% trypsin/1 mM CaCl₂ to obtain single cells. Cells (200,000) in culture medium were plated on 60-mm Petri dishes and incubated on a gyratory shaker at 37°C, 5% CO₂. After 3 h single cells were counted. The cell aggregation data are represented by the index (N₀  N_t)/N₀ where N_t is the single cell number after the incubation time t and N₀ is the cell number at the initiation of incubation.

Rubidium Transport Assay

The ouabain-sensitive ion transport was measured by determining the uptake of ⁸⁶Rb⁺ as described by Lambrecht et al. (1998). The cells were washed once with 1.5 ml of ice-cold wash solution (144 mM NaCl, 0.5 mM CaCl₂, 10 mM HEPES, pH 7.4), incubated for 10 min at 37°C with 1 ml of incubation buffer (144 mM NaCl, 10 mM HEPES pH 7.4, 0.5 mM MgCl₂, 0.5 mM CaCl₂, 1 mM RbCl, 1 mg/ml glucose, 1 µCi ⁸⁶Rb⁺), and then washed three times with 1.5 ml of ice-cold wash solution. To measure the ouabain-sensitive ⁸⁶Rb⁺ transport the cells were incubated with 50 µM ouabain for 30 min at 37°C before the experiment. The cells were dissolved with 500 µl of 0.5 M NaOH for 1 h at RT and solubilized ⁸⁶Rb⁺ was counted. The samples were normalized to the protein content and the ouabain-sensitive Rb flux was calculated.

Sodium Measurement

Monolayers of cells treated without or with 50 µM ouabain for 2 h were rinsed three times with 10 ml of 0.25 M sucrose. The cells of five 100-mm dishes each were pooled in 0.25 M sucrose, digested with HNO₃ (Ultrex II; J.T. Baker, Phillipsburg, NJ) at a final concentration of 40% at 65°C for 15 h, and diluted 1:2 with Millipore Milli-Q UF plus filtered water. The ion concentrations were measured by using an inductively coupled plasma atomic emission spectrometer (Vista Axial 730; Varian, Walnut Creek, CA). The concentrations for Na⁺ (588.995 nm), K⁺ (766.941 nm), and Mg²⁺ (285.213 nm) were determined and the Na⁺ and K⁺ concentrations were normalized to the total Mg²⁺ content (internal control).

	RESULTS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Reduced Levels of E-Cadherin and -Subunit in MSV-MDCK Cells and Establishment of MSV-MDCK Cell Lines Expressing Both E-Cadherin and -Subunit

Immunoblot analysis revealed that MSV-MDCK cells have substantially reduced levels of the Na,K-ATPase ₁-subunit (90% reduction) (Figure 1A, lane 2) relative to MDCK_wt cells (Figure 1 A, lane 1). These cells also have highly reduced E-cadherin levels (Figure 1B, lane 2; Behrens et al., 1989). These results suggested that the invasiveness of MSV-MDCK cells may result in part from diminished levels of Na,K-ATPase -subunit. To test whether reduced ₁-subunit levels are associated with the invasive phenotype of MSV-MDCK cells, we overexpressed ₁-subunit in MSV-MDCK cells and in E-cadherin-expressing MSV-MDCK (MSV-Cad vector) cells. MSV-Cad vector cells express 85% of the total E-cadherin expressed in MDCK_wt cells (Figure 1B, lane 5; Rajasekaran et al., 1996). Canine ₁-subunit cDNA in an eukaryotic expression vector was transfected into MSV-MDCK and MSV-Cad cells. Two stable clones that expressed -subunit in MSV-MDCK (MSV-NaK-cl 1 and cl 2) (Figure 1A, lanes 3 and 4), and two -subunit-expressing MSV-Cad clones (MSV-Cad-NaK-cl 1 and cl 2) (Figure 1A, lanes 6 and 7) were selected by using G418 and hygromycin, respectively. MSV-MDCK cells and MSV-Cad cells transfected with vector lacking the insert (MSV-MDCK vector; Figure 1, A and B, lane 2) and MSV-Cad vector (Figure 1, A and B, lane 5) were selected as controls for transfection. Immunoblot analysis and densitometric quantification revealed that MSV-NaK-cl 1 and MSV-NaK-cl 2 express 40 and 67% of the -subunit level, respectively, compared with MDCK_wt cells. MSV-Cad-NaK-cl 1 and MSV-Cad-NaK-cl 2 were found to express 112 and 43% of the -subunit, respectively, relative to MDCK_wt cells.

View larger version (24K): [in this window] [in a new window]   Figure 1.   Analysis of the expression of Na,K-ATPase - and -subunits and E-cadherin in MDCK and MSV-MDCK cells. Total cell lysates (50 µg) (A) or 100 µg (B and D) were separated on a 10% SDS-PAGE, transferred to nitrocellulose, and immunoblotted for Na,K-ATPase -subunit (A), E-cadherin (B), and -subunit (D) as described in MATERIALS AND METHODS. (C) Analysis of the surface expression of -subunit by a biotinylation assay.

A cell surface biotinylation assay was used to determine the levels of -subunit expressed on the plasma membrane (Figure 1C). The surface -subunit level of MDCK cells was considered as 100% (Figure 1C, lane 1). MSV-Cad-NaK-cl 1 expressed similar levels of surface -subunit compared with MDCK_wt cells (Figure 1C, lane 6) and MSV-Cad-NaK-cl 2 expressed ~60% of -subunit on the surface compared with MDCK_wt cells (Figure 1C, lane 7). MSV-NaK-cl 1 and MSV-NaK-cl 2 cells expressed 30 and 80%, respectively, of the surface -subunit of MDCK_wt cells (Figure 1C, lanes 3 and 4). MSV-MDCK vector and MSV-Cad vector cells expressed ~15% of the surface -subunit of MDCK_wt (Figure 1C, lanes 2 and 5). Cells that contained high levels of total -subunit (MSV-NaK-cl 2 and MSV-Cad-NaK-cl 1) expressed higher levels of surface -subunit (compare lanes 4 and 6 of Figure 1, A and C).

In contrast to the levels of ₁-subunit, the ₁-subunit level did not reveal a drastic reduction in MSV-MDCK cells (60% reduction, Figure 1D, lane 2). The ₁-subunit levels increased 2- to 3-fold in cells overexpressing -subunit (Figure 1D, lanes 3 and 4 and 6 and 7). MSV-MDCK (Figure 1D, lane 2) and MSV-Cad cells (Figure 1D, lane 5) transfected with the vector alone showed similar -subunit levels. This result indicates that repletion of -subunit expression increases the level of -subunit in MSV-MDCK cells.

MSV-MDCK Cells Expressing Both E-Cadherin and -Subunit Show a Polarized Epithelial Phenotype

MSV-MDCK cells expressing both -subunit and E-cadherin grew as compact colonies (Figure 2, E and F) comparable to MDCK_wt cells (Figure 2G). MSV-Cad vector cells (Figure 2B) or MSV-NaK cells (Figure 2, C and D) showed a fibroblastic phenotype and grew as single cells similar to MSV-MDCK vector cells (Figure 2A). In addition, confluent MDCK_wt cells and both clones of MSV-Cad-NaK cells formed honeycomb-like monolayers (Figure 2, E', F', and G'). However, MSV-MDCK, MSV-Cad vector, and MSV-NaK cells grew loosely attached to each other as revealed by increased intercellular space between cells (Figure 2, A'-D'). These results indicated that repletion of E-cadherin and -subunit of Na,K-ATPase expression in MSV-MDCK cells induces an epithelial phenotype.

View larger version (133K): [in this window] [in a new window]   Figure 2.   Phase contrast microscopy of MDCK cells and MSV-MDCK cells expressing E-cadherin and Na,K-ATPase -subunit. Phase contrast micrographs of cells grown at low cell density (A-G) and of confluent monolayers (A'-G'). MDCKwt cells (G) and MSV-MDCK cells expressing E-cadherin and the -subunit of Na,K-ATPase, MSV-Cad-NaK-cl 1 (E), and MSV-Cad-NaK-cl 2 (F) grow as compact colonies at low density and form tight monolayers at confluence (G', E', and F'). MSV-MDCK cells expressing either E-cadherin (B and B') or the -subunit, MSV-NaK-cl 1 (C and C') and MSV-NaK-cl 2 (D and D'), alone grow with a fibroblastic phenotype similar to MSV-MDCK vector cells (A and A'). Bar, 50 µm.

A distinguishing characteristic of the polarized epithelial phenotype is the presence of tight junctions. Immunofluorescence staining of ZO-1, a tight junction protein, revealed a continuous staining pattern in both clones of MSV-Cad-NaK cells (Figure 3, E and F), which was comparable to MDCK_wt cells (Figure 3G). In MSV-MDCK vector and MSV-NaK cells most of the ZO-1 staining was localized intracellularly (Figure 3, A, C, and D). In MSV-Cad vector cells ZO-1 was localized on the plasma membrane, however, the staining pattern was discontinuous (Figure 3B; Rajasekaran et al., 1996), indicating that the presence of E-cadherin alone is sufficient for the initial translocation of ZO-1 to the plasma membrane. Consistent with the ZO-1 staining pattern, transmission electron microscopy revealed tight junctions in MSV-Cad-NaK cells and MDCK_wt cells (Figure 4, A-C, arrows). In addition, these cells also had distinct desmosomes (Figure 4, A-C, arrowheads). MSV-Cad vector cells (Figure 4D) or MSV-NaK cells (our unpublished results) did not show tight junctions. The presence of tight junctions in MSV-Cad-NaK cells was further confirmed by a macromolecular permeability assay. Because MSV-Cad-NaK cells did not grow well on polycarbonate filters we were unable to measure transepithelial electrical resistance, a commonly used method to assess the function of tight junctions. Therefore, we tested the impermeability to macromolecules of confluent monolayers grown on glass coverslips. Anti E-cadherin antibody was added to the apical surface and the tight junction permeability to the antibody was detected by staining the cells with a secondary antibody under nonpermeabilized conditions (Figure 4). MDCK_wt (Figure 4, K and L) cells and both clones of MSV-Cad-NaK cells (Figure 4, G and H and I and J) were impermeable to anti-E-cadherin antibody, indicating the presence of functional tight junctions. In contrast, MSV-Cad cells showed clear E-cadherin staining on the plasma membrane, indicating the absence of tight junctions (Figure 4, E and F).

View larger version (122K): [in this window] [in a new window]   Figure 3.   Immunofluorescence of ZO-1 in MDCK cells and MSV-MDCK cells expressing E-cadherin and Na,K-ATPase -subunit. MSV-MDCK vector (A), MSV-NaK-cl 1 (C), and MSV-NaK-cl 2 (D) cells show ZO-1 mainly localized to the cytoplasm. Expression of E-cadherin in MSV-MDCK cells (B) results in the translocation of ZO-1 to the plasma membrane. However, these cells do not show a continuous ZO-1 ring pattern on the plasma membrane like MSV-Cad-NaK-cl 1 (E), MSV-Cad-NaK-cl 2 (F), and MDCKwt (G) cells. Bar, 10 µm.

View larger version (92K): [in this window] [in a new window]   Figure 4.   Transmission electron microscopy and passage of macromolecules through confluent monolayers of MDCK cells and MSV-MDCK cells expressing E-cadherin and Na,K-ATPase -subunit. MSV-MDCK cells expressing both E-cadherin and the Na,K-ATPase -subunit, MSV-Cad-NaK-cl 1 (A) and MSV-Cad-NaK-cl 2 (B), form tight junctions (arrows) and desmosomes (arrowheads). Tight junctions and desmosomes of MDCKwt cells are shown in C. MSV-MDCK cells expressing only E-cadherin, MSV-Cad vector cells (D), form neither tight junctions nor desmosomes. Bar, A-C, 0.5 µm; bar, D, 2 µm. Confluent monolayers were fixed under nonpermeabilizing conditions with paraformaldehyde. The cells were incubated from the apical side with anti-E-cadherin antibody and FITC-conjugated secondary antibody. The absence of tight junctions in MSV-Cad vector cells results in clear staining of E-cadherin on the plasma membrane (F). In MSV-MDCK cells expressing E-cadherin and Na,K-ATPase -subunit, MSV-Cad-NaK-cl 1 (H) and MSV-Cad-NaK-cl 2 (J), as well as in MDCKwt cells (L), the antibody is not able to pass through tight junction and therefore does not stain the basolaterally localized E-cadherin. E, G, I, and K are phase contrast micrographs of the same areas depicted in F, H, J, and L, respectively. Bar, 10 µm.

The presence of tight junctions correlates with the polarized distribution of apical and basolateral plasma membrane markers in epithelial cells. Three-dimensional images of monolayers of MDCK_wt (Figure 5C), MSV-Cad-NaK (Figure 5, A and B), and MSV-Cad vector (Figure 5D) cells, double-labeled with antibodies against ZO-1 (red) and E-cadherin (green), were generated from serial optical sections obtained by laser scanning confocal microscopy. We showed previously that latitudinal rotation of the image by 56° enables clear visualization of the apical and basolateral regions of the monolayer in MDCK cells (Rajasekaran et al., 1996). MSV-Cad-NaK cells and MDCK_wt cells showed that E-cadherin is distinctly localized to the basolateral membrane. In addition, these cells also showed distinct basolateral localization of Na,K-ATPase - and -subunit and apical localization of GP135, an apical marker (our unpublished results). In contrast, MSV-Cad vector cells were not polarized and E-cadherin was found all over the cell surface (Figure 5D, arrowheads). In MSV-Cad-NaK and MDCK_wt cells the apicolateral staining of ZO-1 can be distinctly separated from the basolateral staining of E-cadherin. In contrast, in MSV-Cad cells, ZO-1 staining colocalized with E-cadherin staining in the apical adherens junction region, indicating that the tight junction components are not separated from the basolateral membrane (Figure 5D; Rajasekaran et al., 1996). These results demonstrated that coexpression of E-cadherin and -subunit of Na,K-ATPase in MSV-MDCK cells results in the establishment of epithelial polarization. Neither of these proteins when expressed alone was sufficient to induce polarization of MSV-MDCK cells.

View larger version (51K): [in this window] [in a new window]   Figure 5.   Confocal microscope images showing the distribution of E-cadherin and ZO-1 in MSV-MDCK cells expressing both E-cadherin and Na,K-ATPase -subunit, MDCK, and MSV-MDCK cells expressing E-cadherin alone. Confluent monolayers of MSV-Cad-NaK-cl 1 (A), MSV-Cad-NaK-cl 2 (B), MDCKwt (C), and MSV-Cad vector (D) cells were grown on glass coverslips and fixed in cold methanol. Cells were labeled with a polyclonal antibody against ZO-1 and a monoclonal antibody against E-cadherin followed by Texas Red-conjugated anti-rabbit and fluorescein-conjugated anti-mouse antibodies. Serial optical sections of the monolayers were recorded at 0.5-µm intervals. Three-dimensional images were created and rotated 56° latitudinally by using Fluoview image analysis software. Note the distinct staining pattern of ZO-1 (red) and E-cadherin (green) in MSV-Cad-NaK-cl 1, MSV-Cad-NaK-cl 2, and MDCKwt cells, and the colocalization of these proteins (yellow) in MSV-Cad vector cells. E-cadherin is also seen above the ZO-1 staining (arrows) in MSV-Cad vector cells. Bar, 5 µm.

Epithelial Phenotype Is Lost in a Reverted Clone of MSV-Cad-NaK-Cells

To further confirm that the phenotypic reversion of MSV-MDCK cells is due to repletion of both E-cadherin and -subunit and not a transfection artifact, MSV-Cad-NaK-cl 1 was maintained in the absence of G418 and hygromycin used to select this clone. This growth condition resulted in a dramatic reduction of both -subunit levels (80% reduction) (Figure 6A) and E-cadherin levels (90% reduction) (Figure 6B) in the reverted clone (MSV-Cad-NaK rev). These cells were fibroblastic and grew like MSV-MDCK vector cells both at low density (Figure 6C) and when at confluence (our unpublished results). In addition, transmission electron microscopy did not show tight junctions in these cells (Figure 6D). These results indicate that the phenotypic reversion of MSV-MDCK cells is due to the near normal levels of both E-cadherin and -subunit and that these levels are crucial to induce and maintain the polarized phenotype of MSV-MDCK cells.

View larger version (82K): [in this window] [in a new window]   Figure 6.   Loss of Na,K-ATPase -subunit and E-cadherin expression in a reverted clone of MSV-MDCK cells expressing Na,K-ATPase -subunit and E-cadherin, and characterization of MSV-MDCK cells expressing E-cadherin and Na,K-ATPase -subunit. (A) Total cell lysates (50 µg) were separated on a 10% SDS-PAGE and immunoblotted for Na,K-ATPase -subunit as described in MATERIALS AND METHODS. Reversion of MSV-Cad-NaK-cl 1 cells (MSV-Cad-NaK rev) results in a drastic reduction of -subunit expression. (B) Total cell lysates (100 µg) were separated on a 10% SDS-PAGE and immunoblotted for E-cadherin. MSV-Cad-NaK rev cells show also a dramatic reduction of E-cadherin protein levels. (C) Loss of E-cadherin and -subunit expression results in the reversion to a fibroblastic phenotype. Bar, 50 µm. (D) Transmission electron microscopy reveals that MSV-Cad-NaK rev cells form neither tight junctions nor desmosomes (arrows). Bar, 0.5 µm. (E) Immunoblot analysis: total cell lysates (100 µg) of MSV-Cad-NaK cells and MDCKwt cells were separated on a 10% SDS-PAGE and immunoblotted for Na,K-ATPase -subunit as described in MATERIALS AND METHODS. (F) Cell surface biotinylation: Cells were biotinylated and immunoblotted for Na,K-ATPase -subunit. (G and H) Immunofluorescence staining of ZO-1. In MSV-Cad-NaK-cl 1 (G) and in MSV-Cad-NaK-cl 2 (H) cells, ZO-1 is localized on the plasma membrane but does not show a continuous ring-like staining pattern. Bar, 10 µm.

Enhanced Expression of the -Subunit Does Not Alter the Fibroblastic Phenotype of MSV-Cad Cells

To test whether overexpression of Na,K-ATPase -subunit induces an epithelial phenotype, MSV-Cad cells were transfected with the canine ₁-subunit cDNA in an eukaryotic expression vector and two stable clones expressing 200 and 100% of the -subunit expressed in MDCK_wt cells were selected (Figure 6E). Cell surface biotinylation experiments revealed that only ~20% of the total -subunit present in these cells was expressed on the cell surface (Figure 6F). Consistent with the biotinylation data, immunofluorescence microscopy revealed that most of the -subunit was localized in large intracellular vesicles (our unpublished results). These results are consistent with published reports indicating that the -subunit is crucial for the surface expression of the -subunit (Geering, 1991; Geering et al., 1996). MSV-Cad-NaK cells were fibroblastic like MSV-Cad vector cells (our unpublished results) and ZO-1 localization in these clones was discontinuous similar to MSV-Cad vector cells (Figure 6, G and H).

Taken together, our results demonstrate that expression of E-cadherin alone is not sufficient to induce epithelial polarity and that the -subunit plays a crucial role in the induction and maintenance of the polarized phenotype of MSV-MDCK cells.

Repletion of -Subunit in MSV-Cad Cells Abolishes Their Invasive Behavior

In MSV-MDCK cells their invasive behavior has been attributed to a reduced level of E-cadherin expression (Behrens et al., 1989). However, in an in vitro collagen gel invasion assay commonly used to monitor the invasiveness of carcinoma cells (Hordijk et al., 1997), we found that MSV-Cad vector cells (Figure 7B) were as invasive as MSV-MDCK vector cells (Figure 7A). These cells grew in a dispersed manner and showed invasion throughout the matrix, indicating that forced expression of E-cadherin alone is not sufficient to inhibit their invasive behavior. MSV-NaK cells (Figure 7, C and D), MSV-Cad-NaK cells (Figure 7E) and MSV-Cad-NaK rev cells (Figure 7F) were invasive and grew in a dispersed manner in the collagen gel. In contrast, the two clones of MSV-Cad-NaK cells grew predominantly as colonies without invading the matrix (Figure 7, G and H), indicating that repletion of both E-cadherin and -subunit inhibits the invasiveness of MSV-MDCK cells.

View larger version (177K): [in this window] [in a new window]   Figure 7.   Invasion of collagen matrix by MSV-MDCK cells and by MSV-MDCK cells expressing E-cadherin and Na,K-ATPase -subunit or Na,K-ATPase -subunit. Single-cell suspensions were plated into a collagen matrix and invasiveness was monitored as described in MATERIALS AND METHODS. MSV-Cad vector (B), MSV-NaK-cl 1 (C), and MSV-NaK-cl 2 (D) cells grow, invading throughout the collagen matrix like MSV-MDCK vector cells (A). Similarly, MSV-Cad-NaK-cl 1 cells (E) and MSV-Cad-NaK rev cells (F) grow, invading throughout the collagen matrix. MSV-Cad-NaK-cl 1 (G) and MSV-Cad-NaK-cl 2 (H) cells grow predominantly as colonies without invading the collagen matrix. Bar, 25 µm.

MSV-MDCK Cells Expressing Both E-Cadherin and -Subunit Show Reduced Motility

Invasive carcinoma cells are characterized by their increased cell motility. Therefore, we determined whether the reduced invasiveness of MSV-Cad-NaK cells is accompanied by a reduced cell motility. Two independent methods, a Transwell cell motility assay (Figure 8A) and a wound assay (Figure 8, B and C) were used to determine the motility. The Transwell cell motility assay clearly showed that MSV-Cad-NaK cells were significantly (p < 0.01) less motile than either MSV-MDCK vector or MSV-MDCK cells overexpressing either E-cadherin or -subunit alone (Figure 8A). Also, MSV-Cad-NaK rev and MSV-Cad-NaK cells were significantly more motile than MSV-Cad-NaK cells. Consistent with the Transwell cell motility assay results, the wound assay also revealed a significantly (p < 0.01) reduced motility of MSV-Cad-NaK cells compared with MSV-MDCK vector, MSV-Cad vector, and MSV-NaK-cells (Figure 8B). MSV-Cad-NaK rev cells and the MSV-Cad-NaK clones, likewise, were significantly more motile than MSV-Cad-NaK cells (Figure 8C). In both motility assays, a correlation between the levels of -subunit expression and cell motility was observed. MSV-Cad-NaK-cl 1 cells that express more -subunit were less motile than MSV-Cad-NaK-cl 2 cells. Similarly, MSV-NaK-cl 2 cells were less motile than MSV-NaK-cl 1 cells. Interestingly, MSV-Cad and MSV-NaK cells were less motile than MSV-MDCK cells transfected with the vector. A two-way analysis of variance comparing logarithms of motile cell numbers confirmed a significant (p < 0.01) effect of E-cadherin or -subunit expression alone and an interaction effect that is greater (p < 0.03) than multiplicative. This suggests that E-cadherin or -subunit expression alone may reduce the motility of carcinoma cells to some extent and that overexpression of both E-cadherin and -subunit acts synergistically to significantly reduce tumor cell motility.

View larger version (22K): [in this window] [in a new window]   Figure 8.   Transwell motility assay and wound assay of MSV-MDCK cells expressing E-cadherin and Na,K-ATPase - or -subunit. (A) Migration of MDCK cells and MSV-MDCK cells expressing E-cadherin and Na,K-ATPase - or -subunit across porous filter membranes. The error bars show the SD of the mean of triplicate determinations done in duplicates. (B) Migration of MSV-MDCK cells expressing E-cadherin and Na,K-ATPase -subunit in the wound assay. Note that MSV-MDCK vector cells closed the wound already after 6 h. The error bars show the SD of the mean of three independent determinations done in quadruplicates. (C) Migration of MSV-MDCK cells expressing E-cadherin and Na,K-ATPase -subunit and of reverted MSV-Cad-NaK cells in the wound assay. The error bars show the SD of the mean of three independent determinations done in quadruplicates.

Expression of -Subunit Results in a More Stable Association of E-Cadherin to the Submembranous Actin Cytoskeleton

An actin-based cytoskeleton regulates epithelial polarization (Jou and Nelson, 1998), cell motility, and invasiveness of transformed epithelial cells (Carlier and Pantaloni, 1997; Hordijk et al., 1997; Keely et al., 1997). Therefore, we determined the organization of actin by FITC-phalloidin staining in MSV-MDCK cells expressing either E-cadherin, -subunit, or both. MSV-Cad-NaK (Figure 9, A and B) and MDCK_wt cells (Figure 9C) showed a continuous submembranous cortical actin compared with MSV-Cad vector cells in which the actin ring was discontinuous (Figure 9D, arrows). MSV-MDCK, MSV-NaK, MSV-Cad-NaK, and MSV-Cad-NaK rev cells showed a discontinuous cortical actin cytoskeleton similar to that of MSV-Cad vector cells (our unpublished results). These results indicated that enhanced expression of both E-cadherin and -subunit in MSV-MDCK cells results in the formation of a cortical actin cytoskeleton similar to MDCK_wt cells.

View larger version (116K): [in this window] [in a new window]   Figure 9.   F-actin and E-cadherin staining and detergent solubility of E-cadherin, - and -subunit in MSV-MDCK cells expressing E-cadherin and Na,K-ATPase -subunit. (A) MSV-Cad-NaK-cl 1 and MSV-Cad-NaK-cl 2 (B) cells show an intense cortical actin ring similar to MDCKwt cells (C). Similarly, E-cadherin localization in MSV-Cad-NaK-cl 1 (E), MSV-Cad-NaK-cl 2 (F), and MDCKwt cells (G) were nearly identical. Overexpression of E-cadherin alone in MSV-MDCK cells did not reveal a similar staining pattern of cortical actin (D) and E-cadherin (H). Bar, A-D, 20 µm; bar, E-H, 10 µm. (I) Confluent monolayers were extracted with Triton X-100 and soluble and insoluble fractions were obtained by centrifugation. Equal volumes of supernatant (S) and pellet (P) fraction were separated on a 10% SDS-PAGE and immunoblotted with anti-E-cadherin antibody and visualized by enhanced chemiluminescence. (J) Relative levels of E-cadherin in soluble and insoluble fractions were quantified by densitometric analysis. Bars indicate the SD of three independent determinations.

We then tested whether the change in the cortical actin cytoskeleton organization resulted in an increased stabilization of E-cadherin on the plasma membrane in MSV-Cad-NaK cells. Immunofluorescence staining of E-cadherin in MSV-Cad-NaK cells (Figure 9, E and F) was almost indistinguishable from MDCK_wt cells (Figure 9G), whereas MSV-Cad vector cells did not show such an E-cadherin-staining pattern (Figure 9H). The observed well-organized cortical actin and intense E-cadherin staining on the plasma membrane suggested that E-cadherin might be more stably associated with the actin cytoskeleton in MSV-Cad-NaK cells compared with MSV-Cad vector cells. E-cadherin function in cell-cell adhesion is dependent on its stable association with the actin cytoskeleton (Hirano et al., 1987; Nagafuchi and Takeichi, 1988). Detergent extraction and quantification of E-cadherin in the soluble and insoluble fractions have been widely used to assess the strength of interaction of E-cadherin with the actin cytoskeleton (Hordijk et al., 1997; Piepenhagen and Nelson, 1998). Triton X-100 extraction of confluent monolayers of MDCK_wt and MSV-Cad-NaK cells revealed that 50-90% of the E-cadherin was detergent insoluble, indicating an increased association with the actin cytoskeleton (Figure 9, I and J, lanes 1, 3, and 4). In contrast, only 23% of E-cadherin was detergent insoluble in MSV-Cad vector cells (Figure 9, I and J, lane 2). Because MSV-Cad vector cells start to pile up after 48 h of growth, the detergent solubility experiments were carried out at this time point. However, in MDCK cells, at 24 and 48 h still a large amount of E-cadherin is extracted in the soluble fraction. In fact, it takes ~5 d to significantly reduce E-cadherin in the soluble fraction of MDCK cells (Shore and Nelson, 1991). However, in MSV-Cad-NaK-cl 1 and MSV-Cad-NaK-cl 2 cells a significant amount of E-cadherin is detergent insoluble at 48 h, indicating that the association of E-cadherin with cortical actin cytoskeleton takes place much faster in MSV-Cad-NaK cells than in MDCK cells. Similarly, - and -subunits of Na,K-ATPase revealed high detergent insolubility in MDCK_wt and MSV-Cad-NaK-clones (our unpublished results). These results suggest that the E-cadherin-mediated cell adhesion machinery is weakly associated with the cortical actin cytoskeleton in cells expressing low levels of Na,K-ATPase -subunit. An increased expression of -subunit in E-cadherin-expressing MSV-MDCK cells appears to increase the strength of the E-cadherin-mediated cell adhesion system, perhaps by stabilizing the association of the E-cadherin complex with the cortical actin cytoskeleton.

Reduced Association of E-Cadherin to the Actin Cytoskeleton Does Not Compromise the Adhesive Behavior of MSV-Cad Cells

We then tested whether the reduced association of E-cadherin to the actin cytoskeleton alters the cell adhesion function of E-cadherin expressed in these cells by using a cell aggregation assay commonly used to monitor the cell-cell contact function of cell adhesion molecules (Nagafuchi and Takeichi, 1988; Lu et al., 1998; Noe et al., 1999). In this assay, a defined number of single cells in growth medium is plated on a plastic Petri dish (which will not support cell attachment) with growth media and incubated on a gyratory shaker at 37°C for few hours. Cells that can adhere will form aggregates, whereas cells that lack this ability will remain as single cells. The number of single cells was determined as described in MATERIALS AND METHODS. MSV-MDCK cells transfected with the vector alone showed no aggregation after 3 h of incubation (Figure 10). In contrast, MSV-Cad, and MDCK_wt as well as both clones of MSV-Cad-NaK cells aggregated similarly to ~95% (Figure 10). The aggregation of MSV-Cad cells was found to be dependent on the presence of calcium because depletion of calcium inhibited the aggregation of these cells. This result indicated that a reduced association of E-cadherin to the actin cytoskeleton does not compromise the calcium-dependent adhesive function of E-cadherin in MSV-Cad cells. Surprisingly, both of the MSV-NaK clones clearly showed increased aggregation compared with the MSV-MDCK vector cells (Figure 10). Because these latter cells lack detectable E-cadherin expression (Figure 1B, lanes 3 and 4) the aggregation observed with these cells should be due to the ectopic expression of the -subunit. Aggregation of MSV-NaK cells is not due to transfection artifacts because MSV-MDCK vector cells that were transfected and selected similarly like MSV-NaK cells did not show any aggregation.

View larger version (30K): [in this window] [in a new window]   Figure 10.   Aggregation of MDCK and MSV-MDCK cells expressing E-cadherin and -subunit of Na,K-ATPase. Single cells (200,000) were plated in regular culture medium in a plastic Petri dish and incubated at 37°C on a gyratory shaker (80 rpm) for 3 h. Note MSV-Cad vector cells aggregated similar to MDCKwt and MSV-Cad-NaK cells. MSV-Cad vector cells were incubated in calcium free medium containing 2.5 mM EDTA to test the calcium dependence of cell aggregation in these cells. Note in the absence of calcium, the aggregation of MSV-Cad vector cells is inhibited by more than 85% compared with cells incubated in the presence of calcium. The aggregation index was calculated as described in MATERIALS AND METHODS. SE bars indicate the average of three independent experiments done in triplicates.

Expression of -Subunit of Na,K-ATPase Reduces the Intracellular Sodium Levels in MSV-MDCK Cells

It seemed possible that MSV-MDCK cells would have reduced Na,K-ATPase activity due to their reduced levels of - and -subunits and that the ectopic expression of the -subunit would increase the Na,K-ATPase activity in these transformed cells. The increased Na,K-ATPase activity resulting from -subunit expression might be involved in the regulation of the polarized phenotype or the decreased cell motility in E-cadherin and -subunit-expressing MSV-MDCK cells. We measured the ouabain-sensitive influx of ⁸⁶Rb⁺ to monitor the Na,K-ATPase activity (Reznik et al., 1985; Lambrecht et al., 1998) as described in MATERIALS AND METHODS. The ⁸⁶Rb⁺ uptake was comparable in MSV-MDCK vector and MSV-Cad vector cells, which express similar levels of - and -subunits. However, in spite of highly reduced levels of Na,K-ATPase - and -subunits (Figure 1) the Na,K-ATPase activity in these cells was only 25% less than that of MDCK_wt cells (p < 0.01) (Figure 11A). Comparison of the ⁸⁶Rb⁺ uptake among MSV-MDCK vector, MSV-NaK-cl 1, MSV-NaK-cl 2, MSV-Cad vector, MSV-Cad-NaK-cl 1, and MSV-Cad-NaK-cl 2 cells did not demonstrate any significant correlation of Na,K-ATPase transport activity with either the -subunit (Spearman rank correlation r_S = 0.26) or -subunit levels (r_S = 0.49).

View larger version (25K): [in this window] [in a new window]   Figure 11.   Analysis of Na,K-ATPase function in MSV-MDCK cells expressing E-cadherin and -subunit of Na,K-ATPase. (A) Ouabain-sensitive 86Rb+ uptake. The 86Rb+ uptake assay was performed as described in MATERIALS AND METHODS and the ouabain-sensitive 86Rb+ flux was normalized to total protein. The bars indicate the SE of two independent determinations done in triplicates. (B) Determination of the intracellular Na+ levels by atomic emission spectrometry. The intracellular sodium concentrations were measured at 588.995 nm and normalized to the Mg2+ content of the cells. The error bars show the SE of the mean of two independent determinations.

Although the ⁸⁶Rb⁺-uptake rates were near normal, the reduced number of sodium pumps on the plasma membrane in MSV-MDCK cells might have resulted in an increased intracellular sodium content, thereby increasing the activity of the enzyme. To test this possibility, we determined the intracellular sodium levels in these different cell lines. To remove the extracellular sodium, the cells were extensively washed in 0.25 M sucrose before analysis. Therefore, the obtained sodium levels should represent the intracellular sodium content ([Na]_i). This analysis revealed an [Na]_i of 12 mM for MDCK_wt cells, which is a reasonable value for intracellular sodium. Treatment of MDCK_wt cells with the Na,K-ATPase inhibitor ouabain increased ([Na]_i by >10-fold and was accompanied by a dramatic decrease in the potassium content of these cells (our unpublished results). Ouabain treatment of MSV-MDCK cells expressing either -subunit or E-cadherin or both increased the sodium content by >10-fold with corresponding decreases in the potassium content (our unpublished results). Thus, the ouabain-sensitive changes in the levels of intracellular sodium and potassium validated our ionic measurements by using atomic emission spectrometry. The sodium levels in MSV-MDCK cells expressing either -subunit or E-cadherin or both -subunit and E-cadherin are shown in Figure 11B. The [Na]_i of MSV-MDCK vector cells was 2.8-fold higher than that of MDCK_wt cells (p < 0.05). The [Na]_i of MSV-Cad vector cells was comparable to that of MSV-MDCK vector cells. In cells transfected with -subunit (MSV-NaK-cl 1 and -cl 2), [Na]_i levels were reduced compared with MSV-MDCK vector cells, but the differences were only borderline statistically significant. Coexpression of E-cadherin and Na,K-ATPase -subunit further decreased [Na]_i compared with MSV-MDCK vector cells (p < 0.05), and this difference appeared larger than the effect due to the expression of -subunit alone. The intracellular potassium levels in all these cell lines were similar (our unpublished results). These results indicate that the Na,K-ATPase -subunit expression in MSV-MDCK cells results in the reduction of the intracellular sodium levels.

	DISCUSSION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Epithelial Polarization Requires E-Cadherin and the -Subunit of Na,K-ATPase

In this study, we demonstrate that Na,K-ATPase ₁-subunit is required for epithelial polarization and suppression of cell invasion and motility in E-cadherin-expressing MSV-MDCK cells. Because MSV-MDCK cells expressing E-cadherin alone were nonpolarized, invasive, and motile, and the expression of Na,K-ATPase ₁-subunit in these cells induced a polarized phenotype, abolished invasiveness, and reduced cell motility, we suggest that kidney epithelial cell polarity and tumor invasion suppression are likely regulated by a functional synergism between E-cadherin and Na,K-ATPase. Our conclusions are based on multiple independent controls that show a relationship between the polarized phenotype and -subunit and E-cadherin expression. 1) Replenishing Na,K-ATPase -subunit expression in two independent clones expressing E-cadherin induces a polarized phenotype and suppresses invasion and motility. 2) Expression of Na,K-ATPase -subunit in two independent clones of E-cadherin-expressing MSV-MDCK cells did not induce a polarized phenotype. 3) E-cadherin-expressing MSV-MDCK cells transfected with vector alone did not show an altered phenotype. 4) Two independent clones of MSV-MDCK cells expressing -subunit alone did not show any change in phenotype. 5) Using a reverted clone, we show that near normal expression levels of E-cadherin and -subunit are crucial to maintain the polarized phenotype and reduce invasiveness and motility of MSV-MDCK cells. Clearly, we recognize that MSV-transformation of MDCK cells may result in multiple genetic and biochemical changes, including loss of E-cadherin and -subunit expression. However, our results indicate that replenishing both E-cadherin and -subunit expression is sufficient to significantly reverse the Moloney sarcoma virus-induced transformed phenotype of MDCK cells.

Overexpression of -subunit in MSV-MDCK and MSV-Cad cells increased the levels and surface expression of the -subunit (our unpublished results). In contrast, ectopic expression of Na,K-ATPase -subunit in MSV-Cad cells resulted predominantly in its intracellular localization. Repletion of -subunit expression in MSV-MDCK and MSV-Cad cells might have resulted in the more efficient transport of the -subunit from the endoplasmic reticulum to the plasma membrane and stabilization of the / heterodimer on the cell surface as previously reported in Xenopus oocytes (Geering, 1991; Geering et al., 1996). MSV-NaK cells that lack E-cadherin expression did not show a polarized phenotype despite higher -subunit levels, indicating that E-cadherin is also crucial for the development of the polarized phenotype. Our results thus indicate that both E-cadherin- and the -subunit-mediated /-heterodimer function is crucial for the development of a polarized phenotype in MSV-MDCK cells.

Lack of tight junctions and desmosomes in MSV-MDCK cells expressing E-cadherin alone and the presence of these junctions in MSV-MDCK cells expressing both E-cadherin and -subunit suggest that E-cadherin's cell-cell adhesion function alone is not sufficient to induce junctional complexes in kidney epithelial cells. In a previous report, we showed that in MSV-MDCK cells ZO-1 is predominantly localized to the cytoplasm. E-cadherin expression in these cells resulted in the translocation of ZO-1 to the plasma membrane. However, these cells showed a discontinuous ZO-1 staining pattern and failed to make functional tight junctions (Rajasekaran et al., 1996). In polarized MDCK cells the adherens junction is localized below the tight junction, and in cells expressing functional tight junctions the ZO-1 staining can be clearly separated from the E-cadherin staining by using three-dimensional laser scanning confocal microscopy. In MSV-Cad cells, ZO-1 colocalizes with E-cadherin in the adherens junction region (Figure 5D; Rajasekaran et al., 1996), indicating the absence of tight junctions. In contrast, the ZO-1 staining can be clearly separated from the lateral E-cadherin staining in MDCK and MSV-Cad-NaK cells (Figure 5, A and B), indicating the presence of tight junctions. These results suggest that functional E-cadherin is essential for initial events that trigger the translocation of ZO-1 to the plasma membrane, whereas the -subunit-mediated /-heterodimer function is crucial for events that regulate the formation of an undisrupted ZO-1 ring, functional tight junctions, and, consequently, the polarized epithelial phenotype. Therefore, we propose that the tight junction development might consist of two major steps: 1) E-cadherin-dependent early signaling events necessary for the targeting of tight junction proteins to the adherens junction region, and 2) E-cadherin-independent events that regulate the separation of tight junction components from the adherens junction region to form functional tight junctions. It is possible that the /-heterodimer itself by strengthening the cell-cell contact or the ionic gradient generated by the Na,K-ATPase (see below) might play a role in the regulation of events that separate tight junction components from the adherens junction region to form functional tight junctions.

Suppression of Invasiveness and Motility of Carcinoma Cells Requires E-Cadherin and the -Subunit of Na,K-ATPase

E-cadherin's association with the actin cytoskeleton is dramatically increased in E-cadherin- and -subunit-expressing MSV-MDCK, indicating that the -subunit expression may directly or through Na,K-ATPase activity strengthen the cell-cell contact mediated by E-cadherin (see below). In addition, these cells show junctional complexes such as tight junctions and desmosomes, which might further increase and stabilize the adhesive contact amon