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Vol. 14, Issue 9, September 2003

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View larger version (61K): [in this window] [in a new window]   Figure 4. Cellular localization of UGT in human cells expressing native GalT-1. Human liver-derived HepG2 cells, which lack GalT-1, were transfected with the UGT construct. UGT-1 labeled by the anti-HA antiserum (B) colocalized with the anti-CTR433 antibody used as a Golgi marker (A, C, and E). In contrast, when UGT was transfected into Caco-2 cells, which express GalT-1, part of the UGT labeled by the anti-HA antiserum displayed a typical ER pattern (D). No labeling of the ER was observed when Caco-2 cells were transfected with the Golgi marker myc-tagged sialyltransferase, which was labeled by an anti-myc antiserum (F). Cells were counterstained with the appropriate FITC- (A, C, and E) and Texas Red-labeled (B, D, and F) secondary antibodies. Bar, 10 µm.

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content