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Vol. 15, Issue 1, January 2004

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In Figure 5 of the aritcle "The Retrieval Function of the KDEL Receptor Requires PKA Phosphorylation of its C-terminus", by M. Cabrera, M. Muñiz, J. Hidalgo, L. Vega, M.E. Martin, and A. Velasco (Mol. Biol. Cell [2003], 14, 4114–4125, a commercial antibody claimed to be specific for ARF-GAP1 was used to show by immunoblot analysis recruitment of this protein to peptides mimicking the cytoplasmic domain of the KDEL receptor. Dr. Dan Cassel from Technion-Israel Institute of Technology brought to our attention that this antibody was actually raised against a different protein known as ARF-GAP3. Nevertheless, we have repeated the experiment with an antibody specific for ARF-GAP1 and have obtained similar results to those reported in the article, indicating that both ARF-GAP1 and ARF-GAP3 interact with a peptide mimicking the phosphorylated state of the cyophasmic tail of the KDEL receptor.

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