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Vol. 17, Issue 1, 263-271, January 2006
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* Dipartimento di Biologia e Patologia Molecolare e Cellulare, Istituto di Endocrinologia ed Oncologia Sperimentale, CNR, 80131 Napoli, Italy;
Divisione di Farmacologia, Dipartimento di Neuroscienze, Università "Federico II," 80131 Napoli, Italy
Submitted September 6, 2005;
Revised October 12, 2005;
Accepted October 18, 2005
Monitoring Editor: Donald Newmeyer
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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; Gray et al., 1998; McKnight et al., 1998; Feliciello et al., 2001; Houslay and Adams, 2003; Tasken and Aandahl, 2004; Taylor et al., 2004; Wong and Scott, 2004). AKAP121 (also called D-AKAP1), AKAP149, and AKAP84 arise from a single gene by alternative pre-mRNA splicing (Lin et al., 1995; Trendelenburg et al., 1996; Chen et al., 1997; Huang et al., 1997, 1999; Furusawa et al., 2002). AKAP121 and AKAP84 tether PKA to the mitochondrial outer surface. This localization is mediated by the interaction of AKAP121 and AKAP84 with 
tubulin, an integral component of mitochondrial outer membrane (Cardone et al., 2002). AKAP121 is widely expressed in several tissues and its accumulation is regulated at the transcriptional level by the cAMP/PKA pathway (Feliciello et al., 1998). Anchoring of PKA to mitochondria supports cAMP signaling and suppresses apoptosis (Harada et al., 1999; Affaitati et al., 2003). AKAP121, via a KH domain at its COOH-terminus, binds at least two mRNAs that encode mitochondrial proteins (Ginsberg et al., 2003; Ranganathan et al., 2005). This multicomponent system, reminiscent of other AKAP complexes at cell membranes, ensures efficient translation and import of nuclear-encoded mitochondrial proteins. It is suggested that PKA may phosphorylate some of these proteins cotranslationally, as well as acting on AKAP121 itself to regulate the stability of the RNA-AKAP121 complex (Ginsberg et al., 2003; Feliciello et al., 2005).
In addition, AKAP121 and AKAP84 bind the central core of PTPD1, a classical nonreceptor protein tyrosine phosphatase (Moller et al., 1994). PTPD1 binds to and activates src, enhancing EGF-dependent mitogenic signaling (Cardone et al., 2004). By translocating PTPD1 to the outer membrane of mitochondria, AKAP121 inhibits PTPD1-dependent EGF signaling to the nucleus. These data suggest a model whereby AKAP121, by targeting PTPD1/src complex to mitochondria, may shift the focus of tyrosine kinase signaling from membrane to specific distal organelles, such as mitochondria (Feliciello et al., 2005).
We tested this hypothesis and found that AKAP121, indeed, targets src tyrosine kinase to mitochondria. By manipulating the localization and expression of AKAP121, we were able to modulate cAMP- and src-dependent signaling to mitochondria, affecting phosphorylation of mitochondrial substrates, activity of components of the respiratory chain, mitochondrial membrane potential (
m) and oxidative synthesis of ATP.
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MATERIALS AND METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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). GC2 cells were grown at 37°C in DMEM supplemented with 10% FCS. Primary cultures of human fibroblasts were obtained from cutaneous biopsy of healthy subjects. Primary human fibroblasts and NIH3T3 cells were propagated in DMEM medium supplemented with 10% calf serum.
Antibodies and Chemicals
Polyclonal anti-AKAP121 antibody was purchased from Santa Cruz (C-20; Santa Cruz, CA). We also used an anti-AKAP121 polyclonal antibody that has been previously described (Chen et al., 1997). Anti-PTPD1 polyclonal antibody was prepared as previously described (Moller et al., 1994). Mouse monoclonal anti-src antibody was purchased from Oncogene Research Products (Boston, MA); anti-COXII from Molecular Probes (Eugene, OR); anti-hemoagglutinin epitope (HA.11) from Covance (Madison, WI); anti-tubulin from Sigma (St. Louis, MO); anti-SOD monoclonal antibody from Walter Occhiena; anti-AKT from Santa Cruz; CPT-cAMP from Sigma; and H89 and PP2 from Calbiochem (La Jolla, CA).
Plasmids and Transfection
Mouse pCEP4-AKAP121 cDNA was a gift of Dr C. Rubin (Albert Einstein College of Medicine, New York). An AKAP84 mutant lacking the first 30 amino acids was generated by PCR using specific oligonucleotides. The PCR product was subcloned in the CMV vector. cDNA coding the kinase-inactive form of Src (Lys259 changed to methionine) was cloned into pSG5 (Barone and Courtneidge, 1996) and was kindly provided by Prof. A. Migliaccio (Second University of Naples, Italy). The vector encoding for human PTPD1 was previously described (Moller et al., 1994). A small DNA insert (
70 base pairs) encoding for short hairpin RNA targeting mouse AKAP121 (nucleotides 301–321, ATG + 1) was subcloned in pRNA-H1/neo vector (GenScript, Piscataway, NJ). Blast search confirmed that this sequence specifically recognizes mouse. A scrambled sequence subcloned in the same vector was used as experimental control. siRNA vectors were transiently transfected using the lipofectamine protocol. All plasmids were purified using QIAGEN tip columns (Qiagen, Chatsworth, CA) and sequenced using the CEQ2000 DNA Analysis System and a Beckman automated sequencer (Fullerton, CA).
Immunoprecipitation and Immunoblot Analysis
Cells were homogenized in lysis buffer (20 mM Tris-HCl, pH 7.4, 0.15 M NaCl, 10 mM EDTA, 0.25% Triton X-100, 0.05% Tween-20, 0.02% sodium azide) containing aprotinin (5 µg/ml), leupeptin (10 µg/ml), pepstatin (2 µg/ml), and 0.5 mM phenylmethylsulfonyl fluoride. The lysates were cleared by centrifugation at 15,000 x g for 15 min. Cell lysates (2 mg) were immunoprecipitated with the indicated antibodies. An aliquot of cell lysate (100 µg) or immunoprecipitates were resolved by SDS-PAGE gel and transferred to Immobilon P membrane. The immunoblot analysis was performed as previously described (Cardone et al., 2004). Chemoluminescent (ECL) signals were quantified by scanning densitometry (Molecular Dynamics, Sunnyvale, CA). Highly purified mitochondria and supernatant fraction were isolated as described (Hovius et al., 1990).
Immunofluorescence Analysis
Cells were rinsed with phosphate-buffered saline (PBS) and fixed in 3.7% formaldehyde for 20 min. After permeabilization with 0.5% Triton X-100 in PBS for 5 min, the cells were incubated with PBS 1x/0.1 mg/ml bovine serum albumin for 1 h at room temperature. Double immunofluorescence was carried out with the following antibodies: anti-superoxide dismutase monoclonal (1/200), anti-AKAP121 goat polyclonal (1/200; Santa Cruz, sc-6439), anti-PTPD1 rabbit polyclonal (1/200), and anti-AKAP121/84 rabbit polyclonal (1/100). Fluorescein- or rhodamine-tagged anti-rabbit and anti-mouse IgG secondary antibodies were used. Coverslips were analyzed by confocal microscopy.
Assays for Cytochrome c Oxidase Activity and Mitochondrial ATP Synthesis
The activity of cytochrome c oxidase on purified mitochondria was determined by spectrophotometric measurement of the rate of reduced cytochrome c oxidation at 550 nm (Couperstein and Lazarov, 1951). Cytochrome c (type VI, Sigma) was reduced by dithiothreitol (DTT) at a final concentration of 0.5 mM. Cytochrome c reduction was assessed measuring A550/A565 ratio. In our conditions, this ratio was between 15 and 20. The mitochondrial fraction was suspended in 60 mM of phosphate buffer (pH 7.4) containing 0.6% lauryl maltoside and centrifuged for 10 min at 10,000 x g. The assay was performed in a total volume of 1 ml of phosphate buffer (60 mM) containing 0.1 mg of mitochondrial proteins, 30 µM final concentration of reduced cytochrome c. The decrease in absorbance at 550 nm was measured for 1 min with 15 seconds integration time (Stieglerova et al., 2000).
Assay for Oxidative ATP Synthesis
Cells were harvested by trypsinization 48 h after transfection, washed twice in PBS, and counted in a hemo-cytometer. A replicate for each sample was prepared that had been treated for 1 h with 4 µg/ml rotenone (Sigma). The emission recorded from samples treated with rotenone was defined as baseline luminescence corresponding to a nonmitochondrial source of ATP. Assays were performed using the ATP luminescence assay kit HS II (Roche, Nutley, NJ) according to manufacturer's instructions, using 3000 cells per sample. Light emission was recorded in a single measure of 2 s using a Lumat LB 9507 luminometer (Berthold Technologies, Bad Wildbad, Germany).
Quantitative Analysis of Mitochondrial DNA
Mitochondrial DNA content relative to nuclear genome from control (CMV) and AKAP121 expressing cells was evaluated by PCR using oligonucleotide primers specific for nuclear ( globin) and mitochondrial genes (NADH dehydrogenase and cytochrome b). The primer sequences used were: cytochrome b: FW: 5' CCTAGGCGACCCAGACAATTAT; rev: 5'-TCATTCGGGCTTGATGTGG; NADHd: FW: 5'-CAGCCATTCTCATCCAAACC; rev: 5'-ATTATGATGCGACTGT GAGTGC; globin: FW: 5-AGCCTGACCAACATGGTGAAAC; rev: 5'-AGCCACCTGAATAGCTGGGACT. PCR reactions were carried out on the Applied Biosystem 7000 Real time PCR System (Foster City, CA) using Syber Green method. All reactions were performed in a 30-µl mixture containing 1x SYBR reaction buffer, 10 µM primers, and 20 ng of whole cellular DNA. PCR products range between 70 and 150 base pairs. Southern blot analysis was performed as described (Maniatis, 1989). Briefly, 20 µg of total genomic DNA was digested with EcoRI restriction enzyme, separated on 1% agarose gel, transferred to N-Hybond membrane, and sequentially hybridized with the mitochondrial and nuclear ( globin) cDNA probes. The mitochondrial probe spanning the nucleotides 7392–8625 of human mitochondrial genome was obtained with the following oligonucleotide primers: FW: 5' GGATGCCCCCCACCCTAC; rev: 5'-GGAGGTGGGGATCAATAGAGG.
Imaging Mitochondrial Membrane Potential
The m was assessed using the fluorescent dye tetra-methyl rhodamine ethyl ester (TMRE) in the "redistribution mode." Cells transfected with CMV and AKAP121 were loaded with TMRE, 20 nM, for 30 min in a medium containing 156 mM NaCl, 3 mM KCl, 2 mM MgSO4, 1.25 mM KH2PO4, 2 mM CaCl2, 10 mM glucose, and 10 mM HEPES. The pH was adjusted to 7.35 with NaOH (Abramov et al., 2004). At the end of the incubation, cells were washed in the same medium containing 20 nM dye and allowed to equilibrate. A decline of mitochondria-localized intensity of fluorescence was indicative of mitochondrial membrane depolarization. Confocal images were obtained using a Zeiss inverted 510 confocal laser scanning microscopy (Thornwood, NY) and a 63x oil immersion objective. The illumination intensity of 543 Xenon laser, used to excite TMRE fluorescence, was kept to a minimum of 0.5% of laser output to avoid phototoxicity.
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RESULTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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). We analyzed src localization in GC2 cells by double immunostaining with anti-AKAP121 and anti-src antibody. The signals were collected and analyzed by confocal microscopy. As expected, AKAP121 selectively localized on mitochondria (Figure 2b) (Cardone et al., 2004). Some src immunostaining overlapped that of AKAP121 (Figure 2, a–c), suggesting that both proteins colocalize on mitochondria. Similarly, src immunostaining partly overlapped that of superoxide dismutase (Figure 2, d–f), a protein that selectively localizes to the mitochondrial matrix. However, src staining was also evident at the cell periphery (membrane) as well as at the perinuclear and nuclear regions, where no mitochondria or AKAP121 were detectable. We confirmed this finding using different anti-AKAP121 or anti-src antibodies (our unpublished data; see Materials and Methods). We performed similar experiments examining src localization in human fibroblast primary cultures that express endogenous AKAP121. Growing fibroblasts were labeled in vivo with Texas red-conjugated mitotracker, which selectively accumulates into mitochondria, fixed in formaldehyde, and immunostained with anti-src antibody. Src staining partly overlapped with that of mitotracker (Figure 2, g–i). Additionally, we asked whether exogenous AKAP121 expression in the HEK293 cell line, which costitutively expresses low AKAP121 levels, would target src to mitochondria. Indeed, HEK293 cells stably transfected with AKAP121 showed significant amounts of src concentrated on mitochondria (Figure 2, j–l).
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The ability of AKAP121 to direct src to mitochondria was also demonstrated in HEK293 cells by cofractionation experiments. HEK293 were transiently transfected with expression vectors encoding PTPD1 and AKAP121. Forty-eight hours after transfection, mitochondrial and supernatant fractions were isolated, size-fractionated on SDS-PAGE, and immunoblotted with the indicated antibodies. As shown in Figure 3, AKAP121 copurified with the mitochondria-enriched fraction, as did the mitochondrial voltage-dependent anion channel (VDAC), whereas tubulin and MAPK were found exclusively in supernatant fractions. In untransfected control cells most of endogenous c-src protein was found in the supernatant. Expression of AKAP121, PTPD1, or both significantly increased the amount of c-src recovered in the mitochondrial fraction. Note that coexpression of AKAP121 and PTPD1 translocates an amount of src to mitochondria roughly equivalent to AKAP121 or PTPD1 alone. PTPD1 and src are not only localized to mitochondria. Significant amounts of these enzymes have been found associated with other organelles and cell structures. This suggests that interaction with specific targeting sites is critically dependent on the absolute levels and binding affinity of AKAP121, src, and PTPD1.
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AKAP121 Enhances src-dependent Phosphorylation and Activation of Mitochondrial Substrates
Some src is normally found in the mitochondrial matrix, where it phosphorylates and stimulates the activity of cytochrome c oxidase (COX), a component of the mitochondrial respiratory chain (Miyazaki et al., 2003). We wanted to determine if src localization by AKAP121/PTPD1 on mitochondria correlated with enhanced phosphorylation of mitochondrial src substrates. HEK293 cells were transiently transfected with AKAP121 and PTPD1 vectors for 24 h and subsequently harvested and lysed. Mitochondrial fractions were prepared and subjected to immunoblot analysis with anti-phosphotyrosine antibody. As shown in Figure 4A, expression of AKAP121 or PTPD1, to a lesser degree, markedly enhanced tyrosine phosphorylation of mitochondrial proteins. In contrast to src localization, stimulation of tyrosine phosphorylation by AKAP121 required src activity. Thus, treatment with PP2 or expression of src K– reduced phosphorylation to control levels. The phosphorylation of some mitochondrial substrates in cells transfected with AKAP121 and PTPD1 was reduced, compared with cells expressing AKAP121 alone. We have evidence that PTPD1 is not only localized to mitochondria, but in the absence of AKAP121, a significant fraction is linked to the actin cytoskeleton. In this compartment, PTPD1 regulates EGF-dependent src-FAK signaling (Carlucci, Gedressi, Avvedimento, Garbi, and Feliciello, unpublished results).
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1–30). This mutant retained the ability to bind PTPD1/src and PKA, but failed to target these proteins to mitochondria (Cardone et al., 2004). AKAP841–30 was transiently transfected in HEK293 cells, and COX activity was assayed. The expression of mutant protein was comparable to that of wild-type AKAP121 (our unpublished data). Figure 4B shows that AKAP841–30 acted as dominant negative, reducing COX activity by ca. 40% relative to controls. Stimulation of COX activity by AKAP121 depended on active src kinase. Treatment of the transfected cells with PP2 reduced COX activity below control levels. The amount of COX protein, as shown by Western blot, was unaffected by transfection and treatment with PP2 (Figure 4B, inset). This indicates that the number of mitochondria per cell remained constant during these treatments.
Elevated oxidative respiratory chain activity is associated with increased mitochondrial DNA content (Deveaud et al., 2004). We therefore asked whether AKAP121 promoted mitochondrial DNA accumulation. Using semiquantitative PCR, we monitored the accumulation of two mitochondrial genes, NADH dehydrogenase and cytochrome B. The nuclear globin gene was used as an internal control. Figure 4C shows that AKAP121 increased the levels of NADH dehydrogenase and cytochrome B by ca. 50%, compared with control cells transfected with the CMV vector (CMV). To confirm this finding, we performed Southern blot analysis on total cellular DNA using as probes mitochondrial and globin cDNAs. Control cells and cells expressing AKAP121 were serum-deprived overnight, transferred to 10% FCS, and harvested at the indicated times. As shown in Figure 4D, AKAP121 increased both basal and serum-stimulated mitochondrial DNA levels by 2–3-fold.
AKAP121 Regulates m and Oxidative ATP Synthesis
Electron flux through the respiratory chain is used to reduce free oxygen at the level of complex IV. Hydrogen then moves from the mitochondrial matrix to the intermembrane space. This generates an electrochemical gradient, expressed as m (Saris and Carafoli, 2005). The m is fundamental for the efficient production of ATP and requires the coordinated activity of several enzyme complexes, including COX. Because AKAP121-src stimulated COX activity, we asked if this anchor protein enhanced the m under basal or stress conditions using a fluorescence assay (Abramov et al., 2004). Indeed, mitochondria in HEK293 transiently transfected with AKAP121 were hyperpolarized in comparison to control cells (Figure 5A, a and d). Hyperpolarization was inhibited by 30-min exposure to H89 (10 µM), an inhibitor of PKA and other kinases (Figure 5Ae). Similarly, treatment with PP2 (10 µM) for 30 min reduced polarization (Figure 5Af). Treatment with H89 or PP2 also decreased the intensity of mitochondria localized fluorescence in control cells (Figure 5, A and B). Similar results were obtained in HEK293 cells stably transfected with AKAP121 (Figure 5C).
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m in control cells (Figure 6A, b and c, and B). In contrast, these treatments only slightly reduced the intensity of mitochondria localized fluorescence in AKAP121-transfected cells (Figure 6A, e and f, and B). The localized fluorescence intensity of cells expressing AKAP121 was significantly higher than in control cells.
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; Ginsberg et al., 2003). Taken together, these data imply that PKA up-regulates ATP synthesis and this effect is enhanced by AKAP121. ATP accumulation was also dependent on src activity. Thus, ATP concentrations were lowered by PP2 in both control and, more dramatically, in AKAP121-transfected cells. Western blot analysis showed that neither PP2 nor H89 reduced expression of AKAP121 (our unpublished data).
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We then examined the effects of serum deprivation on ATP accumulation. Twenty-four hours after transfection, cells were serum-deprived for 18 h and their mitochondrial ATP concentration was determined. Serum-deprivation significantly reduced ATP levels in control cells and in cells expressing AKAP121. These findings, along with the data reported in Figure 6B, suggest that growth factors stimulate oxidative phosphorylation and that AKAP121 increases mitochondrial robustness and resistance to oxidative stresses.
To confirm that AKAP121 regulates the mitochondrial respiratory chain, we silenced endogenous AKAP121 by DNA vector-based siRNA and measured ATP levels. As control, we used the same vector carrying a scrambled RNA sequence. Mouse fibroblasts (NIH3T3), which express AKAP121, were transiently transfected and the efficiency of silencing was evaluated by immunoblot analysis (Figure 7B). Expression of siRNAAKAP121 decreased endogenous AKAP121 levels ca. 2.5-fold. The control vector, which expresses siRNAscrambled, did not reduce AKAP121 concentrations. Consistent with our hypothesis, the synthesis of mitochondrial ATP was suppressed by siRNAAKAP121 but not siRNAscrambled. The extent of inhibition by siRNA was comparable to that provoked by src inhibition with PP2 (Figure 7C).
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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m, and ATP synthesis were enhanced by AKAP121 in an src- and PKA-dependent manner.
AKAPs, by colocalizing signaling enzymes and their substrate(s), are proposed to ensure efficient propagation of transduction events generated at distal sites to specific intracellular compartments (Rubin, 1994; Feliciello et al., 1997; Gray et al., 1998; McKnight et al., 1998; Feliciello et al., 2001; Houslay and Adams, 2003; Tasken and Aandahl, 2004; Taylor et al., 2004; Wong and Scott, 2004). AKAP121 clearly plays this role in mitochondria. By localizing PKA at the outer membrane of mitochondria, AKAP121 increases PKA-dependent phosphorylation/inactivation of proapoptotic protein BAD and enhances cell survival (Harada et al., 1999; Affaitati et al., 2003). AKAP121 also facilitates PKA-dependent phosphorylation and activation of StAR, a mitochondrial steroidogenic factor that localizes in mitochondria of adrenal and testicular Leydig cells. In doing so, AKAP121 increases the biosynthesis of steroid hormones in a PKA-dependent manner (Stocco, Dyson, Jones, and Gottesman, unpublished results).
Signaling enzymes other than PKA are also bound and targeted by AKAP121. The amino-terminus of AKAP121 interacts with the central core of PTPD1, localizing the phosphatase on mitochondria (Cardone et al., 2004). PTPD1 is an effector for EGF signal transduction from the membrane to the nucleus. AKAP121 binding diverts PTPD1 to mitochondria and down-regulates this transduction pathway. We report here that AKAP121, via PTPD1, targets src to mitochondria. AKAP121 enhances src-dependent tyrosine phosphorylation of some mitochondrial substrates, facilitating the mitochondrial respiratory chain and increasing ATP synthesis. This conclusion is based both on AKAP121 overproduction and AKAP121 knockdown by siRNA.
The importance of tyrosine kinase signaling in mitochondrial function is supported by several lines of evidence (Abram and Courtneidge, 1999; Ko et al., 2002; Boerner et al., 2004; Augereau et al., 2005; Salvi et al., 2005). Tyrosine phosphorylation of mitochondrial proteins is stimulated in vitro by ATP and H2O2 (Augereau et al., 2005). ATP production at state 3 likewise enhances phosphorylation; this stimulation is ablated by PP2. Phosphorylation and activation of COX by mitochondrial src is postulated to play an important role in osteoclast function and bone remodeling (Miyazaki et al., 2003). The 39-kDa subunit of complex I is tyrosine-phosphorylated, and subunits of complexes II, III, and IV may also be tyrosine kinase substrates (Augereau et al., 2005). Platelet-derived growth factor (PDGF) signaling is linked to tyrosine phosphorylation of the c and 
subunits of the mitochondrial ATP synthase complex. This accounts for the enhanced activity of ATP synthase seen in a variety of PDGF-treated cells, including cortical neurons, mouse fibroblasts, and kidney cells (Evtodienko et al., 2000; Ko et al., 2002; Boerner et al., 2004). Moreover, serum deprivation has been linked to loss of mitochondrial respiratory control (Gottlieb et al., 2002).
Components of the respiratory chain can be also phosphorylated and regulated by PKA (Yang et al., 1998; Ludwig et al., 2001, Papa et al., 2002). A functional interplay between cAMP, tyrosine kinase, and mitochondrial COX has been recently described (Lee et al., 2005). These authors found that high cAMP levels induced phosphorylation of COX subunit I at tyrosine304 and inhibited COX activity. The responsible tyrosine kinase has not been identified.
We propose that AKAP121 is a nodal point where PKA and src signaling integrate, increasing the rate and magnitude of signaling to mitochondria. The mechanism by which src bound to AKAP121-PTPD1 phosphorylates substrates located within the mitochondrial matrix is still unknown. One possibility is that src may translocate inside mitochondria through the outer/inner mitochondrial transport system (Endo et al., 2003). AKAP121 increases the absolute levels of src anchored at the outer membrane of mitochondria. This facilitates transport of src, as well as PKA, inside the organelle, where both kinases normally reside (Yang et al., 1998; Miyazaki et al., 2003; Papa et al., 2003). This regulation is critical for mitochondrial physiology and explains the essential role of AKAP121 in cell survival, steroidogenesis, and oxidative phosphorylation. Identification of the critical mitochondrial substrates of PKA and src, and the functional relationship between these two signaling enzymes on mitochondria will, of course, require further study.
In this article we also present evidence that mitochondrial DNA content is increased by AKAP121. In this regard, our preliminary data suggest that cAMP and PKA are functionally linked to this process. In Saccharomyces cerevisiae, mitochondrial activity (citric acid cycle and oxidative respiration) and mtDNA content are coregulated. Mitochondrial activities and mtDNA are down-regulated by growth in glucose, whereas growth in a nonfermentable carbon source stimulates oxidative phosphorylation and increases mtDNA content. The cAMP/PKA signal transduction pathway positively regulates both reactions (Robertson et al., 2000; Cho et al., 2001; Griffioen and Thevelein, 2002). We have shown that AKAP121 concomitantly regulates mtDNA content and oxidative ATP synthesis. We cannot, however, ascertain which of the two events is the primary regulator of mammalian mitochondrial metabolism.
In summary, we demonstrate for the first time that AKAP121 regulates src events on mitochondria and highlight a unique role of this protein in the regulation of oxidative metabolism (Figure 7D). This mechanism increases the complexity of the symbiotic relationship developed million years ago between primordial eukaryotic cells and the aerobic bacteria that are thought to be mitochondrial ancestors. In view of the ubiquitous expression of AKAP121, our findings reveal an efficient mechanism that may be used in most or all mammalian cells to adapt physiologically to rapid changes in carbon source availability.
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ACKNOWLEDGMENTS |
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Footnotes |
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Address correspondence to: Antonio Feliciello (feliciel{at}unina.it).
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REFERENCES |
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