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Vol. 17, Issue 12, 5381-5389, December 2006
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Department of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712
Submitted June 19, 2006;
Revised September 28, 2006;
Accepted October 5, 2006
Monitoring Editor: Carole Parent
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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). Together they assemble clathrin triskelia onto membranes to construct a clathrin-coated pit (ter Haar et al., 2000; Brodsky et al., 2001). Four heterotetrameric adaptor proteins have been identified; each is thought to function at a different cellular location (Kirchhausen, 1999). At the plasma membrane the heterotetrameric protein AP-2 recruits clathrin and initiates the assembly of clathrin triskelia into lattices (Gallusser and Kirchhausen, 1993; Owen et al., 2000). Recently, monomeric assembly proteins have been identified at the plasma membrane that could also regulate clathrin traffic. The monomeric assembly protein, AP180, was first purified from coated vesicles of bovine brain (Ahle and Ungewickell, 1986), although a nonneuronal homologue of AP180, CALM, was identified more recently (Dreyling et al., 1996; Tebar et al., 1999). AP180 binds directly to clathrin and promotes assembly of clathrin triskelia into cages of uniform size (Ahle and Ungewickell, 1986; Prasad and Lippoldt, 1988; Ye and Lafer, 1995b).
Analysis of mutants of the AP180 orthologues in Drosophila and Caenorhabditis elegans shows that AP180 regulates synaptic vesicle size as well as the sorting of synaptic proteins such as synaptobrevin (Zhang et al., 1998; Nonet et al., 1999; Bao et al., 2005). In mammalian cells, reduction of AP180 results in irregular clathrin lattices, demonstrating an important role for AP180 in the assembly of clathrin into geometrically precise coated vesicles (Meyerholz et al., 2005). Furthermore, recent studies show that AP180 is involved in the internalization of some receptors like the EGF receptor, but not of others such as the transferrin receptor (Huang et al., 2004). In yeast, the role of AP180 in clathrin-mediated endocytosis is less clear. Deletion of both genes encoding the AP180 orthologues (yAP180a and yAP180b) show no defects in any clathrin-mediated processes (Huang et al., 1999).
In vitro experiments show that AP180 binds clathrin and phosphoinositides such as PIP2 and promotes clathrin assembly on lipid monolayers (Ford et al., 2001). Binding of AP180 to PIP2 is mediated by an NH2-terminal homology domain called the ANTH (AP180 N-terminal homology) domain that is conserved in all members of the AP180 family (Norris et al., 1995; Ye et al., 1995; Hao et al., 1997; Ford et al., 2001; Mao et al., 2001). Other endocytic proteins such as epsin have at their amino terminus a structurally similar ENTH domain (epsin N-terminal homology). Interestingly, binding of ENTH-domain–containing proteins such as epsin, to PIP2 induces curvature of a lipid monolayer, whereas AP180 fails to do so (Ford et al., 2002; Stahelin et al., 2003). This points to important mechanistic differences between various protein components of the endocytic machinery.
Here we examined the intracellular role of AP180 in the social amoeba, Dictyostelium discoideum. Although Dictyostelium AP180 colocalized with clathrin on the plasma membrane of wild-type cells, AP180 null cells displayed a normal distribution of clathrin on the plasma membrane. However AP180 knockouts were deficient in osmoregulation mediated by the contractile vacuole, a process where clathrin is also a key regulator. Collectively our results suggest that AP180 is a clathrin assembly protein with unique contributions to the regulation of contractile vacuole size.
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MATERIALS AND METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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) supplemented with 0.6% penicillin-streptomycin (GIBCO BRL, Gaithersburg, MD) at 20°C on Petri dishes. Clathrin heavy-chain mutants were derived from Ax2 wild-type cells, and clathrin light-chain mutants were derived from the wild-type axenic strain NC4A2 (Niswonger and O'Halloran, 1997a; Wang et al., 2003). Both mutant strains were grown in HL-5 media supplemented with 5 µg/ml blasticidin (Calbiochem, EMD Biosciences, La Jolla, CA).
Cloning of clmA and GFP-AP180 Construct
The clmA gene encoding the AP180 gene product was identified from a Dictyostelium genome database (www.dictybase.org) using a BLAST search (tBLASTn) with the first 300 amino acids of the mammalian neuronal AP180. Predicted protein domains at GeneDB identified an ANTH domain in the first 300 amino acids. Alignment and analysis of the predicted Dictyostelium AP180 protein sequence with protein sequences from other members of the AP180 family were performed using the Megalign program (DNAStar, Madison, WI). The percent identity between the Dictyostelium AP180 and those of other species was determined using the ClustalV parameters. A cDNA for the clmA gene was amplified using the PCR with primers selected from the genomic sequence (DDB0218102), 5'GGATCCATGTCGACACCAT GGGGAAAAGC3' and 5'CCCGGGCTCGAGTATTTAAAAGTAAATATTTTGAAC CTTTTGTTGTTG3'. The 2.1-kb amplified product was subcloned into the pTX-GFP expression vector (Levi et al., 2000) at the BamHI and XhoI sites. This plasmid, pTX-GFP-AP180, was then introduced into cells by electroporation and transformants were selected in HL-5 medium supplemented with 10 µg/ml G418 (geniticin; GIBCO BRL, Grand Island, NY).
Protein Expression and Generation of AP180 Polyclonal Antibody
The amplified AP180 cDNA was subcloned into the glutathione-S-transferase bacterial expression vector pGEX-2T (Smith and Johnson, 1988) using the BamHI and SmaI sites. GST-AP180 was transformed into Escherichia coli BL-21 cells, and the expressed protein was purified from bacteria lysates as previously described (O'Halloran and Anderson, 1992a). The purified protein was used to raise rabbit polyclonal antisera against AP180 (Cocalico Biologicals, Reamstown, PA).
Disruption of clmA by Gene Replacement
A 1.3-kb fragment from the 5'coding sequence of clmA was cloned into the pSP72-Bsr vector (Wang et al., 2002), a derivative of pBluescriptII that encodes a 1.4-kb gene for blasticidin resistance, using the BamHI and XbaI sites. Similarly, a 1.6-kb fragment from the 3' coding sequence of clmA was cloned into the pSP72-Bsr vector using the HindIII and XhoI sites. The two clmA fragments flanking the blasticidin (Bsr)-resistant gene cassette had 20 nucleotides missing from the clmA coding sequence, which were replaced by the Bsr gene. The resulting vector, pSP72-Bsr-AP180 was linearized with BamHI and XhoI and transformed into wild-type Ax2 cells via electroporation. Transformed cells were diluted in HL-5 media supplemented with 5 µg/ml blasticidin and plated in 96-well plates. Resulting clones were screened for the absence of clmA gene by PCR and verified for the absence of the AP180 protein by Western blot analysis.
Western Blot Analysis, Endocytosis Assay, and Differential Fractionation
Samples for Western blotting were prepared by resuspending cells in hot sample buffer and running 1 x 106 cells/lane on a 10% SDS polyacrylamide gel. The gel was transferred onto a nitrocellulose membrane (0.2 µm, Bio-Rad, Hercules, CA) and probed with a 1:2000 dilution of our rabbit anti-AP180 polyclonal antibody followed by a goat anti-rabbit Ig-HRP. Signal was detected using an ECL kit (Pierce Biotechnology, Rockford, IL).
For the fluid-phase uptake assay, 2 mg/ml FITC-Dextran (mw 70 kDa, Sigma-Aldrich, St. Louis, MO) was added to 3 x 106 cells/ml growing in HL-5 suspension cultures. Sodium azide (0.02%) was added to a control flask. To stop uptake of FITC-Dextran, cells were chilled on ice. Samples were taken at 0-, 15-, 30-, 60-, 90-, and 120-min time points and centrifuged at 1100 rpm at 4°C for 5 min. Cells were washed twice and resuspended in HL-5 containing 0.02% sodium azide and kept on ice until all samples were collected. All samples were centrifuged at 1100 rpm at 4°C for 5 min, and the pellet was resuspended in cold Na2HPO4 buffer. The cells were lysed with 20% Triton X-100, and fluorescence uptake was analyzed immediately using a Bio-Rad VersaFluor fluorometer. A sample of the lysate was taken after the addition of Triton X-100 and assessed for protein concentration using Bio-Rad protein Assay (Bio-Rad, Hercules, CA).
Differential centrifugation experiments were performed according to Wang et al. (2003). Briefly, cells were collected and washed in isolation buffer [(10 mM MES, pH 6.5, 50 mM KC2H3O2, pH 6.5, 0.5 mM MgCl2, 1 mM EGTA, 1 mM DTT, and 0.02% NaN3) with 1% protease inhibitors (Fungal Protease Inhibitor cocktail, Sigma-Aldrich, St. Louis, MO) and then lysed through a 0.5 µim polycarbonate membrane (GE Osmonics, Trevose, PA) fitted in a Gelman Luer-Lock-style filter (Gelman Sciences, Ann Arbor, MI). The cell lysates were then centrifuged at 3000 x g for 10 min at 4°C, and the resulting post-nuclear supernatant (PNS) was subjected to 100,000 x g ultracentrifugation for 60 min at 4°C to produce a high-speed supernatant (HSS) and a high-speed pellet (HSP; Wang et al., 2003).
Fluorescence Microscopy
Cells expressing GFP-AP180 (2 x 106 cells/ml) were allowed to attach on coverslips for 15 min at room temperature and washed briefly with PDF buffer (2 mM KCl, 1.1 mM K2HPO4, 1.32 mM KH2PO4, 0.1 mM CaCl2, 0.25 mM MgSO4, pH 6.7) and then overlaid with thin layer of 2% agar NA (Amersham Biosciences, Uppsala, Sweden; Fukui et al., 1987). For imaging the contractile vacuole, the agar layers were incubated in water. Cells were then fixed in 1% formaldehyde in methanol for 5 min at –20°C followed by two washes with phosphate-buffered saline (PBS), rinsed briefly with distilled water, and mounted on microscope slides with mounting media (MOWIOL, Calbiochem, EMD Biosciences, La Jolla, CA). The slides were allowed to dry overnight in the dark and analyzed the following day. For imaging clathrin on the contractile vacuole, we filmed live wild-type and AP180 null cells expressing clathrin light chain tagged with GFP in water. For colocalization studies, clathrin light-chain antibody (Wang et al., 2003) was prepared for immunofluorescence microscopy by preabsorption as follows. Clathrin light-chain mutant cells were grown to a density of 2 x 108 cells/ml and centrifuged at 1500 rpm for 5 min, and the cell pellet was resuspended in 2% formaldehyde in PBS. The cells suspension was incubated for 5 min at room temperature and then centrifuged at 2000 rpm for 5 min. The cell pellet was resuspended in 1% formaldehyde in methanol, incubated at –20°C for 5 min and then centrifuged at 2000 rpm for 5 min. The cell pellet was resuspended in 1.5 ml of 3% bovine serum albumin (Fisher Scientific, Fair Lawn, NJ) in PBS with 0.02% NaN3. Anti-clathrin light-chain serum was added to prepared cells at a 1:5 dilution and incubated at 4°C overnight. The antibody-cell suspension was centrifuged for 10 min at 2000 rpm, and the supernatant was added to the pelleted clathrin light-chain mutant cells and incubated at 4°C overnight for another round of preabsorption. This was repeated at least five times to ensure efficient absorption of nonspecific antibodies from the clathrin light-chain antibody serum. Preabsorbed clathrin light-chain antibody was added to the fixed cells and incubated for 1 h at 37°C in the dark. Cells were washed four times with PBS and incubated with Texas Red–conjugated goat anti-rabbit IgG antibody (30 µg/ml; Molecular Probes, Eugene, OR) for 1 h at 37°C in the dark. Cells were washed four times with PBS, rinsed briefly in water, and mounted on microscope slides as described above. To stain the actin cytoskeleton, wild-type Ax2 and AP180 null cells were allowed to attach to coverslips for 10 min at room temperature and then were fixed in 3.7% formaldehyde in PBS for 20 min at room temperature followed by permeabilization with 0.2% Triton X-100 in PBS for 5 min at room temperature. The cells were then incubated with Texas Red phalloidin (1 U/ml; Molecular Probes) in PBS for 20 min at room temperature. The cells were then washed twice with PBS and mounted on slides as described above.
Microscopy and Confocal Imaging
Cells were imaged using differential interference contrast microscopy and fluorescence microscopy on a Nikon Eclipse TE 200 microscope (Dallas, TX). GFP and Texas Red filters were used. Images were acquired on a Photometrics cooled CCD camera (Tucson, AZ), processed using Metamorph 5.0 software (Universal Imaging, West Chester, PA), and adjusted for better contrast using Photoshop 7.0 (Adobe Photosystems, San Jose, CA). When visualizing the contractile vacuole, exposure times for differential interference contrast (DIC) and GFP fluorescence were kept to a minimum because the activity of the contractile vacuole is sensitive to light. Fluorescent images from Nikon compiled into QuickTime movies (Apple, Cupertino, CA) were taken at 3-s intervals and played at 6 frames/s. Images of fruiting bodies during development were captured on a Zeiss SemiSR microscope (Thornwood, NY) with a 2.0x objective and using NIH image software. Confocal Z-series images (0.4 µm sections) of Ax2 cells expressing GFP-AP180 were obtained from Leica scanning laser confocal microscope (TCS-SP2; Deerfield, IL) and processed using Leica software.
For the quantification of AP180 association with the contractile vacuole, wild-type cells and clathrin light-chain mutant cells expressing GFP-AP180 were analyzed. Cells were fixed and flattened as described above and imaged under DIC and fluorescence optics. Contractile vacuoles were identified using the DIC images, and the presence of GFP-AP180 outlining most of the contractile vacuole was scored using fluorescent images.
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RESULTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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; Ye et al., 1995; Hao et al., 1997). Using the amino acid sequence for the ANTH domain of the mammalian neuronal AP180 protein (Ahle and Ungewickell, 1986), we searched the database of the Dictyostelium genome and found a single gene that showed high homology to this sequence. Analysis of the retrieved sequence showed a conserved lysine-rich motif 28KATx6PKxKH at the amino-terminus, which agreed with the ANTH domain consensus sequence (K/G)A(T/I)x6(P/L/V)KxK(H/Y) (Kay et al., 1999; Ford et al., 2001). The first 300 amino acids at the amino-terminus shared significant homology with the ANTH domain of the AP180 orthologues from D. melanogaster (Lap; 32.3%; Zhang et al., 1998); C. elegans (UNC-11; 28.7%; Nonet et al., 1999); H. sapiens (CALM; 32.7%; Tebar et al., 1999); the neuronal isoform from Bos taurus (AP180; 34%; Ahle and Ungewickell, 1986; Kohtz and Puszkin, 1988); and Saccharomyces cerevisiae (YAP180; 20%; Wendland and Emr, 1998). Analysis of the amino acid sequence carboxyl-terminal to the ANTH domain revealed a clathrin box, LINFD (amino acids 360–364; Morgan et al., 2000), closely followed by an AP-2 binding motif, DPF (amino acids 407–409; Hao et al., 1999; Owen et al., 1999, 2000; Traub et al., 1999) and an NPF motif (amino acids 459–461), which confers binding to EH-domain–containing proteins such as Eps15 (Iannolo et al., 1997; de Beer et al., 1998; Figure 1). Because of the amino acid sequence homology and the presence of all signature motifs common to AP180 family members, including the nonneuronal mammalian AP180 ortholog, CALM, we named this gene clmA. ClmA encoded a protein predicted to contain 695 amino acids with a molecular mass of 79.7 kDa. A BLAST search of the Dictyostelium genome database located clmA to chromosome 3.
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). In addition to the vacuoles, GFP-AP180 also decorated the tubules of the contractile network that fed into the main vacuole (Figure 2B). Costaining fixed cells with an anti-clathrin antibody revealed that these vacuoles contained punctae of clathrin and of GFP-AP180 (Figure 2C).
Clathrin Mutants Display an Altered Distribution of AP180
To test whether clathrin was required for the clustering of AP180 into punctae on the plasma membrane, we expressed GFP-tagged AP180 in cells that lacked either the clathrin heavy-chain gene or the clathrin light-chain gene (Ruscetti et al., 1994; Wang et al., 2003; Figure 3A). In clathrin heavy-chain null cells, the GFP-AP180 punctae at the plasma membrane remained visible; however, most of the cytoplasmic punctae were lost. In general, punctae on the membrane of clathrin heavy-chain null cells were not as bright and were more diffuse than those of wild-type cells. In most clathrin light-chain null cells, the GFP-AP180 remained associated with punctae on the plasma membrane and scattered throughout the cytoplasm, a distribution similar to wild-type cells. In 
20% of cells that lacked clathrin light chain, GFP-AP180 accumulated on one side of the plasma membrane, a pattern never seen in wild-type cells (Figure 3A, inset).
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Clathrin Localization on the Plasma Membrane Is Not Affected in Cells That Lack AP180
Members of the AP180 family associate with clathrin and assemble clathrin triskelia into cages in vitro (Ahle and Ungewickell, 1986; Ye and Lafer, 1995a). To test whether Dictyostelium AP180 is required for the association of clathrin with cellular membranes, we constructed AP180 null cells using homologous recombination to replace a portion of the coding sequence of the clmA gene with a blasticidin marker. Replacement within the clmA gene was confirmed by PCR and the absence of the AP180 protein in AP180 null mutants was verified by Western blot analysis (data not shown). To test whether Dictyostelium AP180 is required for the association of clathrin with cellular membranes, we assessed the distribution of clathrin in wild-type and AP180 null cells using an antibody against clathrin light chain and immunofluorescence microscopy. As shown previously in wild-type cells, clathrin localized as punctae at the plasma membrane, cytoplasm, and perinuclear region (Damer and O'Halloran, 2000). This localization pattern was unchanged in cells that lacked AP180 (Figure 4A). To examine directly the association of clathrin with intracellular membranes, we performed differential cell fractionation. In both wild-type cells and AP180 null cells, clathrin fractionated into the high speed (100,000 x g) pellet that contains membranes (Figure 4B). These results suggested that clathrin retained its ability to associate with intracellular membranes even in the absence of AP180.
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, 1997a; Wang et al., 2003). To examine fluid-phase endocytosis, we compared the ability of wild-type cells and AP180 null cells to internalize a fluid-phase marker, FITC-Dextran. We found that AP180 null cells were able to internalize FITC-Dextran as well as wild-type cells (Figure 6A). Both clathrin heavy-chain and clathrin light-chain mutants also display defects in development as shown by their inability to form fruiting bodies (Niswonger and O'Halloran, 1997a; Wang et al., 2003). In contrast, AP180 mutants formed robust fruiting bodies at the same rate as wild-type cells (Figure 6B). AP180 null cells grew well in suspension cultures without forming multinucleated cells, indicating that cytokinesis was also normal in these mutants (data not shown). In addition, imaging cells stained with Texas-Red–labeled phalloidin showed that wild-type and AP180 null mutants shared a similar organization of the actin cytoskeleton (Supplementary Figure 1S).
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). Similar to the response of AP180 null cells, clathrin light-chain mutants also formed enlarged contractile vacuoles when shifted to a hypo-osmotic environment. Like those of AP180 null cells, the contractile vacuoles in clathrin light-chain mutants were able to fill and discharge, but they grew to a much larger size than the contractile vacuoles of wild-type cells.
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). This marker allowed us to visualize the dynamics of the contractile vacuole in real time in a hypo-osmotic environment. We shifted wild-type cells and AP180 null cells expressing Dajumin-GFP from culture media to water and imaged active contractile vacuoles as they filled and discharged their contents. The contractile vacuole of wild-type cells filled quickly, reached its maximum size, and then discharged to the extracellular environment. Examination of contractile vacuole activity in wild-type cells showed an average cycle time of 44 ± 6.9 s (n = 16; Figure 9). In contrast, the activity of the contractile vacuoles of AP180 null cells persisted. After expanding to their maximum diameter, the enlarged contractile vacuoles in AP180 null cells lingered near the plasma membrane, stretched the membrane, and then fused and discharged their contents. Examination of contractile vacuole activity in AP180 mutants showed that these contractile vacuoles filled with kinetics similar to wild-type cells. However, because these contractile vacuoles became much larger and were delayed in fusion, the total cycle from filling to expulsion was 90 ± 15.6 s (n = 12), twice as long as the cycle displayed by wild-type cells (Figure 9).
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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Relationship between Clathrin and AP180 on the Plasma Membrane
The assembly of clathrin triskelions into ordered lattices on membranes is thought to be promoted by assembly proteins that bind to the plasma membrane through their interactions with phosphoinositides and specific cargo. In vitro assembly studies clearly show that AP180 binds PIP2 and that AP180 can efficiently assemble clathrin triskelia into lattices (Lindner and Ungewickell, 1992; Morris et al., 1993; Ye et al., 1995; Ye and Lafer, 1995b; Ford et al., 2001). In Dictyostelium clathrin heavy-chain null cells, AP180 was clustered into punctae on the plasma membrane. This association of AP180 with the plasma membrane in the absence of clathrin heavy chain is likely driven by the interaction between the ANTH domain of AP180 and the phosphoinositides at the plasma membrane or through interactions between binding motifs within AP180 for other adaptor proteins that reside at the plasma membrane such as AP-2 or EH-domain-containing proteins. The punctae for AP180 suggests that these other proteins might cross-link AP180 even in the absence of clathrin.
Reconstitution of clathrin-budding reactions with lipids and purified AP180, epsin, and clathrin show that AP180 is essential for binding clathrin to the liposomes, whereas epsin drives curvature of the lattice (Ford et al., 2001, 2002). In contrast, our study of living Dictyostelium AP180 null cells showed that clathrin could assemble into punctae on the plasma membrane even in the absence of AP180. Because the Dictyostelium genome contains only a single gene for AP180, other clathrin assembly proteins must drive clathrin assembly on the plasma membrane of AP180 null cells.
AP180 Null Cells Are Osmosensitive
The contractile vacuole system functions in osmoregulation, a particularly important adaptation for protists exposed to continuous osmotic changes in their environment. This organelle consists of an interconnected meshwork of tubules and bladders that fill with water, fuse with the plasma membrane and then contract to discharge water to the extracellular milieu. Normally, wild-type cells growing in culture media contain a few moderately active contractile vacuoles that maintain the osmotic balance of the cell. When the extracellular environment changes from iso-osmotic to hypo-osmotic, the number of contractile vacuoles and their activity increase to cope with increased osmotic pressure and thus prevent the cell from swelling and bursting. Among the fascinating features of the contractile vacuole is its ability to fill the bladder to a discrete size every time it goes though the cycle of expanding and contracting. The mechanism for the control of size for the contractile vacuole bladder is not known. As the bladder fills with water, the tubules connected to the bladder shorten as they are incorporated into the expanding bladder. After the emptying of the bladder, the tubules elongate to regenerate the contractile vacuole system and repeat the cycle (Gerisch et al., 2002; Heuser, 2006).
Clathrin is required for a functional contractile vacuole because clathrin heavy-chain mutant cells contain a dispersed contractile vacuole system without tubules and are osmosensitive (O'Halloran and Anderson, 1992b). Similarly, clathrin light-chain null cells are also osmosensitive and display abnormally large contractile vacuoles (Wang et al., 2003). Our results suggest that AP180 and clathrin cooperate in the cycle of contractile vacuole activity because of two observations: 1) the contractile vacuole phenotype shared between AP180 null cells and clathrin light-chain null cells; and 2) AP180 and clathrin are each found at the contractile vacuole. Both clathrin light chain and AP180 null cells show enlarged contractile vacuole bladders and prolonged contractile vacuole cycles. These deficiencies could be caused by defective clathrin lattices assembled without AP180 on the contractile vacuole. AP180 null cells continued to target clathrin on the contractile vacuole; indeed, clathrin localized even more prominently on the contractile vacuoles of AP180 null cells. This increase in clathrin could reflect an increased time for clathrin assembly without AP180, in view of in vitro studies that AP180 increases the efficiency of clathrin assembly into lattices (Hao et al., 1999). It is also possible that clathrin forms imperfect lattices on the contractile vacuoles of AP180 null cells, as shown recently for mammalian cells (Meyerholz et al., 2005). If clathrin vesicles must form efficiently into structured lattices on the contractile vacuole for full function, then inefficient clathrin assembly could account for the delayed cycle of the contractile vacuole seen in AP180 null cells.
How do clathrin and AP180 contribute to contractile vacuole function? One possibility is that AP180 null cells fail to sort proteins that are important for fusion of the contractile vacuole. The mechanism for the fusion of the Dictyostelium contractile vacuole with the plasma membrane is not known, but conceivably the fusion of this organelle could be regulated by SNARE proteins. In synapses, AP180 is thought to selectively retrieve a synaptic vesicle v-SNARE, synaptobrevin, into clathrin-coated vesicles, and AP180 mutants do not localize this v-SNARE properly (Nonet et al., 1999; Bao et al., 2005). By analogy, AP180 could function in Dictyostelium by retrieving a v-SNARE important for contractile vacuole fusion. In the absence of AP180, the regenerating contractile vacuole might lack sufficient v-SNARES for efficient fusion and consequently would expand to an abnormally large size. Alternatively, the presence of clathrin and AP180 assembled into punctae on the contractile vacuole suggests that AP180-associated coated vesicles function on the contractile vacuole membrane itself, perhaps by remodeling and preparing the contractile vacuole membrane so that it can fuse with the plasma membrane and efficiently discharge its contents.
The finding that clathrin light-chain null cells showed a diminished association of AP180 with the contractile vacuole may seem paradoxical. The standard view is that assembly proteins bind to the plasma membrane first and then recruit clathrin. However the decrease in AP180 localization on the contractile vacuole of clathrin light-chain cells suggests that clathrin could also influence AP180 distribution on membranes. It is possible that coated pits are built by the dynamic interaction of clathrin triskelions and AP180 proteins, as each recruits the other to stabilize the growing clathrin lattice. Without light chain, clathrin triskelia are crippled in function (Wang et al., 2003), and perhaps these compromised triskelia are unable to stabilize AP180 on the contractile vacuole. Thus the interplay between AP180 and clathrin to build a stable and regular lattice on this membrane could be impaired in clathrin light-chain null cells. Clearly, our results support current views that different clathrin-mediated trafficking pathways require different adaptor proteins.
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ACKNOWLEDGMENTS |
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Footnotes |
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The online version of this contains supplemental material at MBC Online (http://www.molbiolcell.org). 
This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-02-0144) on October 18, 2006.
Address correspondence to: Theresa J. O'Halloran (t.ohalloran{at}mail.utexas.edu)
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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) internalized FITC-Dextran at the same rate as wild-type cells (
). (B) Development of wild-type cells (Ax2) and AP180 null cells. Cells were plated on a lawn of E. coli. Following depletion of bacteria, wild-type (Ax2) and AP180 null cells differentiated into fruiting bodies consisting of a robust stalk topped by a round sorus. Scale bar, 0.5 mm.
