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Vol. 17, Issue 2, 966-976, February 2006
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Department of Structural Analysis, National Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan
Submitted July 19, 2005;
Revised November 28, 2005;
Accepted November 29, 2005
Monitoring Editor: Martin A. Schwartz
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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-catenin and that MAGI-1 bound to a guanine nucleotide exchange factor for Rap1, PDZ-GEF1. Depletion of MAGI-1 suppressed the cell-cell contact-induced Rap1 activation and the VE-cadherin-mediated cell-cell adhesion after Ca2+ switch. In addition, relocation of vinculin from cell-extracellular matrix contacts to cell-cell contacts after the Ca2+ switch was inhibited in MAGI-1-depleted cells. Furthermore, inactivation of Rap1 by overexpression of Rap1GAPII impaired the VE-cadherin-dependent cell adhesion. Collectively, MAGI-1 is important for VE-cadherin-dependent Rap1 activation upon cell-cell contact. In addition, once activated, Rap1 upon cell-cell contacts positively regulate the adherens junction formation by relocating vinculin that supports VE-cadherin-based cell adhesion. |
INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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).
VE-cadherin has an extracellular domain constituted by five cadherin domains, a transmembrane domain, and a cytoplasmic domain connected to p120 catenin and -catenin (Iyer et al., 2004). Through -catenin, VE-cadherin is linked to 
-catenin that is associated with the actin cytoskeleton, which results in the maintenance of cell-cell adhesion in conjunction with cytoskeleton (Herren et al., 1998; Navarro et al., 1998; Kobielak and Fuchs, 2004). Tyrosine-phosphorylated VE-cadherin in its cytoplasmic domain provides docking sites for signal-transmitting molecules (Esser et al., 1998; Zanetti et al., 2002; Hudry-Clergeon et al., 2005). Conversely, cytoplasmic domain modified by phosphorylation or associated with signaling molecules triggers the inside-out signal that regulates the VE-cadherin-mediated cell adhesion (Nwariaku et al., 2004). -catenin binds to other signaling molecules including PI3-K and MAGUK with inverted domain structure-1 (MAGI-1) as well as -catenin (Kotelevets et al., 2005).
MAGI-1 consists of six PSD95/DiscLarge/ZO-1 (PDZ) domains, a guanylate kinase domain and two WW domains flanked by the first and second PDZ domain (Dobrosotskaya et al., 1997). Because PDZ domains are docking domains for PDZ-binding molecules, MAGI-1 associates with a variety molecules such as NMDA (N-methyl-D-aspartate) receptors, PTEN, BAI-1, 
-catenin, mNET1, and -catenin (Hirao et al., 1998; Ide et al., 1999;Mino et al., 2000; Dobrosotskaya, 2001). These MAGI-1-associating molecules function at cell-cell contacts (Laura et al., 2002). MAGI-1, therefore, functions as a scaffold molecule by localizing to cell-cell contacts. Recently, MAGI-1 is reported to biochemically form a complex with E-cadherin and -catenin (Kawajiri et al., 2000). However, the role of the E-cadherin/-catenin-MAGI-1 complex in cell-cell junctional formation remains elusive.
Rap1 regulates cell-cell adhesion as well as cell-extracellular matrix (cell-ECM) adhesion (Bos, 2005). We have previously demonstrated that Epac-Rap1 signaling enhances VE-cadherin-dependent cell adhesion, thereby stabilizing vascular endothelial cell junctions (Fukuhara et al., 2005). On cell-cell contact, C3G, a guanine nucleotide exchange factor (GEF) for Rap1, is involved in the signaling mediated by E-cadherin and nectin in epithelial cells (Hogan et al., 2004; Fukuyama et al., 2005). Rap1 cycles between GDP-bound inactive form and GTP-bound active form; Rap1-specific GEFs and GTPase activating proteins (GAPs) activate and inactivate Rap1, respectively. Rap1 GEF family consists of C3G (RAPGEF1), PDZ-GEF1 (RAPGEF2), PDZ-GEF2, CalDAG-GEF1, Epac, and Epac2 (Bos et al., 2001).
We here investigate the involvement of MAGI-1-PDZ-GEF1 in the activation of Rap1 on vascular endothelial cell contact and demonstrate that MAGI-1 recruited to cell-cell junctions by associating -catenin contributes to cell-cell contact-dependent activation of Rap1. In addition, the MAGI-1-mediated signal evoked upon cell-cell contact augments VE-cadherin-dependent endothelial cell adhesion. Thus, engagement of VE-cadherin activates Rap1 via MAGI-1, resulting in positive regulation of VE-cadherin-mediated cell adhesion.
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MATERIALS AND METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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). Adenoviruses encoding Rap1GAPII and LacZ were obtained from S. Hattori (The Institute of Medical Science, University of Tokyo) and M. Matsuda (Research Institute for Microbial Disease, Osaka University, Osaka, Japan), respectively. Endothelial cells were infected with adenovirus at the appropriate multiplicity of infection for more than 24 h before imaging. The coding sequences of human MAGI-1b (hereafter MAGI-1) and PDZ-GEF1 were amplified by PCR using human heart cDNA library as a template and resultant DNAs were inserted into p3x FLAG-CMV-10 (Sigma, St. Louis, MO) and pEGFP-C1 (Clontech, Palo Alto, CA). cDNAs encoding truncated MAGI-1 as indicated in Figures 3B and 4A were similarly inserted into pEGFP-C1. pCALwL-FLAG-C3G, a FLAG-tagged mammalian expression vector, was obtained from M. Matsuda (Research Institute for Microbial Disease, Osaka University, Osaka, Japan; Ohba et al., 2001). pIRM21-PDZ5 expressed FLAG-tagged PDZ domain 5 of MAGI-1 and internal ribosomal entry site-driven dsFP593 (Nagashima et al., 2002). HcRed-p120 catenin-expressed HcRed-tagged p120 catenin was described previously (Kogata et al., 2003).
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-catenin from BD Bioscience (San Jose, CA); anti-ZO-1 from Zymed (South San Francisco, CA), Alexa 488- or Alexa 546-labeled secondary antibodies from Molecular Probes (Eugene, OR); horseradish peroxidase-coupled goat anti-mouse and anti-rabbit IgG from Amersham Biosciences.
Cell Culture and Transfection
Human umbilical vein endothelial cells (HUVECs) and human arterial endothelial cells (HAECs) were purchased from Kurabo (Kurashiki, Japan). The cells were maintained in HuMedia-EG2 with a growth additive set as described previously (Nagashima et al., 2002). Bovine aortic endothelial cells (BAECs), MDCK and 293T cells were maintained in DMEM (Nissui, Tokyo, Japan) supplemented with 10% fetal bovine serum and antibiotics (100 µg of streptomycin and 100 U of penicillin/ml). Endothelial cells and 293T cells were transfected by LipofectAMINE plus reagent (Invitrogen, Carlsbad, CA) and by the calcium phosphate method, respectively.
FRET Imaging and Fluorescence Imaging
HUVECs cultured on collagen-coated glass-base dishes were infected with Adeno-Raichu-Rap1 or transfected with pRaichu-Rap1. The structure of Raichu-Rap1 and the principle of FRET are illustrated as in Figure 1A. Cells were imaged on an Olympus IX-81 inverted fluorescence microscope (Lake Success, NY) as described previously (Nagashima et al., 2002). Dual images for cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) were obtained through an XF1071 excitation filter, an XF2034 dichroic filter, and an XF3075 emission filter for CFP and an XF 3079 for YFP (Omega Scientific, Tarzana, CA), respectively. The ratio image of YFP/CFP were created by MetaMorph 5.0 software (Universal Imaging, West Chester, PA) and displayed as an intensity-modulated display image as described previously (Nagashima et al., 2002).
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Calcium Switch
HUVECs serum-starved for 10 h in medium 199 (Invitrogen) containing 1% bovine serum albumin (BSA) were transiently exposed to 4 mM EGTA for 30 min to chelate extracellular calcium and disrupt Ca2+-dependent intercellular junctions (Volberg et al., 1986). After washing, the cells were allowed to recover in complete cell calcium-containing culture media for the time indicated in the figure (Figures 1D, 6, and 7).
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). Briefly, cells starved in medium 199 containing 1% BSA for 10 h were subjected to a calcium switch or stimulated with 10 µg/ml VE-cadherin ectodomain-Fc (VEC-Fc) protein for the time indicated at the top of the figure (Figure 1E). The cells were lysed at 4°C in pulldown lysis buffer (20 mM Tris-HCl [pH 7.5], 100 mM NaCl, 10 mM MgCl2, 1% Triton X-100, 1 mM EGTA, 1 mM dithiothreitol, 1 mM Na3VO4, 1x protease inhibitor cocktail). Precleared lysates were incubated with GST-Rap1 binding domain of RalGDS precoupled to glutathione-Sepharose beads. Proteins collected on the beads were subjected to SDS-PAGE followed by immunoblotting with anti-Rap1 antibody.
Immunocytochemistry and Confocal Imaging
Cells cultured on glass-bottom dishes were fixed with 2% formaldehyde in phosphate-buffered saline (PBS) for 30 min and permeabilized with 0.1% Triton X-100 for 10 min. Cells were blocked with 3% BSA for 30 min and incubated with anti-MAGI-1b, anti-VE-cadherin, anti-ZO-1, anti-vinculin, or anti--catenin antibody for 1 h at room temperature. Immunopositive reaction was visualized with Alexa 488- or Alexa 546-labeled secondary antibodies. Confocal images were obtained by an Olympus BX50WI microscope controlled by Fluoview.
Immunoprecipitation Assay
HUVECs were lysed with lysis buffer (100 mM NaCl, 50 mM Tris-HCl [pH 7.5], 1% Triton X-100, 2 mM Na3VO4, 1x protease inhibitor cocktail). Precleared cell lysates by centrifugation at 15,000 x g were incubated with antibodies. Immunoprecipitates collected on protein A- or G-Sepharose were subjected to SDS-PAGE and immunoblotting with antibodies as indicated in the figure (Figures 2D, 3, C, D, and E, and 4C).
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Cell Adhesion Assay
Recombinant VEC-Fc chimeric protein was prepared as described previously (Fukuhara et al., 2005). Twenty-four-well tissue culture plates were coated with 10 µg VEC-Fc or Fc protein/ml in PBS-Ca2+/Mg2+ overnight at 4°C followed by blocking with 1% heat-inactivated BSA in PBS (inactivated at 85°C for 12 min) for 1 h at room temperature. HUVECs treated with control siRNAs or MAGI-1 siRNAs were cultured for 48 h and then suspended in 0.5% BSA-containing Medium 199. Resuspended cells, 2.0 x 105, were plated and adhered onto each VEC-Fc- or Fc-coated well at 37°C for the indicated time. To analyze cell adhesion to a collagen-covered surface, a collagen-coated 24-well plate (Asahi Technoglass, Chiba, Japan) was used instead of the VEC-Fc-coated plate. After washing with PBS-Ca2+/Mg2+ four times to remove nonadherent cells, adherent cells and input cells were quantified by measuring endogenous alkaline phosphatase (ALP) activity by using Atto-Phos AP fluorescent substrate system (Promega, Madison, WI).
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RESULTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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; Wittchen et al., 2005). However, it remains elusive where Rap1 is activated on cell contacts. To monitor the spatiotemporal activation of Rap1 on vascular endothelial cell-cell contact, HUVECs expressing Raichu-Rap1 were time-lapse FRET-imaged. Raichu-Rap1 consists of YFP, Rap1, the Ras-binding domain of Raf, CFP, and a CAAX box of Ki-Ras. The intramolecular binding of GTP-Rap1 to Raf induces FRET from CFP to YFP (Figure 1A), whereas the dissociation of Rap1 from Raf reduces FRET. Increased FRET indicated by a red hue was observed at cell-cell contacts during spontaneous movement (Figure 1B and Supplementary Movie 1). Rap1 was constantly activated in the perinuclear region of the cells irrespective of cell-cell contact.
In vascular endothelial cells, the peripheral membrane of cells contacting each other was overlapped. Thus, AJs and TJs are intermingled (Dejana, 2004). To ascertain Rap1 activation at the adherens junctions, HUVECs expressing both Raichu-Rap1 and HcRed-tagged p120 catenin were imaged (Figure 1C). Most of Rap1 activation as indicated by red hue was observed at AJs where p120 catenin was localized. No remarkable Rap1 activation was detected within the protruding membrane overlapping region.
We further quantitatively examined whether the Rap1 is activated during cell adhesion after de-adhesion by chelating extracellular calcium and restoring calcium (hereafter, calcium switch). GTP-bound Rap1 was rapidly increased within 5 min and to a greater extent than the predisruption level by restoration of Ca2+ (Figure 1D, top panel). The quantitative results obtained from three independent experiments were shown (Figure 1D, bottom panel). These results suggest that the cell-cell contact triggers the Rap1 activation in a manner dependent on extracellular Ca2+. Although Rap1 is reported to be activated in a manner dependent on nectin, which is independent of extracellular Ca2+ (Fukuyama et al., 2005), we assumed that Ca2+-dependent cell-cell contact triggers Rap1 activation besides nectin-triggered Rap1 activation. We, therefore, examined the VE-cadherin engagement-dependent Rap1 activation. To mimic the VE-cadherin engagement in nascent cell-cell contacts, we used VEC-Fc chimeric protein, which consisted of the extracellular domain of VE-cadherin fused to the Fc portion of Ig. GTP-bound Rap1 was increased when cells were treated with VEC-Fc, but not with control Fc (Figure 1E).
To examine the requirement of VE-cadherin for Rap1 activation upon cell-cell contact, we imaged Rap1 activation in VE-cadherin-depleted HUVECs. Quantitative FRET imaging analysis upon cell-cell contacts demonstrated that Rap1 activation at the cell-cell contacts was less in VE-cadherin-depleted cells than those observed in control siRNA-treated cells (Figure 1F). Collectively, these data indicate that the engagement of VE-cadherin induces Rap1 activation.
MAGI-1 Localizes to Cell-Cell Contacts and Binds to -Catenin
MAGI-1 constitutes a complex with E-cadherin/-catenin and associates with a GEF for Rap1, PDZ-GEF1 (Kawajiri et al., 2000), implying that MAGI-1 may link the cadherin-mediated signal to PDZ-GEF1 for the activation of Rap1. To investigate the involvement of MAGI-1 in Rap1 activation on VE-cadherin-mediated cell-cell contact, we first developed an anti-MAGI-1 antibody and examined the expression of MAGI-1 in vascular endothelial cells. MAGI-1 was expressed in all cultured vascular endothelial cells we tested, because it was found in MDCK epithelial cells used as a positive control (Figure 2A). Next, we examined the localization of MAGI-1 in vascular endothelial cells by immunostaining. MAGI-1 was localized to the cell-cell contacts (Figure 2B) and colocalized with VE-cadherin (Figure 2C). The immunopositive reaction in the nucleus appeared to be non-specific, because it was detected in the nucleus by immunostaining using preabsorbed anti-MAGI-1 (unpublished data) and after knockdown of MAGI-1 (see Figure 5B).
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-catenin. We examined this link by immunoprecipitation assay (Figure 2D). -catenin bound to both VE-cadherin (Figure 2D, top panel) and MAGI-1 (Figure 2D, bottom panel). These results indicate that MAGI-1 appears to localize to VE-cadherin-based cell adhesion through -catenin in vascular endothelial cells.
MAGI-1 Interacts with PDZ-GEF1 in Vascular Endothelial Cells
It has been shown that MAGI-1 binds to PDZ-GEF1 localized to cell-cell contacts in epithelial cells (Dobrosotskaya and James, 2000; Kawajiri et al., 2000). We hypothesized that PDZ-GEF1 is associated with MAGI-1 in vascular endothelial cells and that it is involved in the activation of Rap1 on VE-cadherin-mediated cell-cell contact. PDZ-GEF1 was expressed in vascular endothelial cells similarly to MAGI-1 (Figure 3A). The interaction between MAGI-1 and PDZ-GEF1 was examined by the immunoprecipitation using the full-length and the truncated mutants of MAGI-1 (Figure 3, B and C). The PDZ-GEF1 bound to the N-terminus of MAGI-1 (Figure 3C). EGFP-tagged MAGI-1 coimmunoprecipitated PDZ-GEF1, but not other GEFs for Rap1, C3G, Epac1, and CalDAG-GEF-I (Figure 3D). We further examined the interaction between endogenous MAGI-1 and PDZ-GEF1 in HUVECs. Both MAGI-1 and PDZ-GEF1 were coimmunoprecipitated from the lysate of HUVECs (Figure 3E), indicating that PDZ-GEF1 associates with MAGI-1 in vascular endothelial cells.
Localization of MAGI-1 to Cell-Cell Contact is Important for Rap1 Activation on Cell Contact
To understand the role of MAGI-1 in activating Rap1 when forming cell-cell contacts, we proceeded to investigate the localization of MAGI-1 using EGFP-tagged MAGI-1 in motile endothelial cells. EGFP-MAGI-1 was accumulated at cell-cell contacts (Figure 4B, left panels, and Supplementary Movie 2). Removal of the carboxy terminal PDZ domain (delta PDZ5) resulted in the dissociation of MAGI-1 from cell-cell contacts (Figure 4B, right panels, and Supplementary Movie 3). Because it was reported that MAGI-1 binds to -catenin through PDZ5 (Dobrosotskaya and James, 2000), we tested the requirement of PDZ5 for the association of MAGI1- with -catenin. -catenin was coimmunoprecipitated with EGFP-tagged full-length MAGI-1 but not with MAGI-1 lacking PDZ5 (Figure 4C). These results suggest that MAGI-1 localizes to vascular endothelial cell-cell contacts in a manner dependent on -catenin. We further revealed that MAGI-1 was dislocated from the cell-cell contact of the PDZ5-expressing cells as marked by red fluorescence but not from that of wild-type cells (Figure 4D), indicating that PDZ5 is important for the localization of MAGI-1 to cell-cell contacts.
To examine the requirement of the association of MAGI-1 with -catenin for the cell-cell contact-induced Rap1 activation, we checked the effect of disconnection of MAGI-1 to -catenin by overexpressing MAGI-1 PDZ domain 5 on Rap1 activation. In HUVECs expressing MAGI-1 PDZ domain 5, as marked by dsFP593 (Figure 4E and Supplementary Movie 4), Rap1 activation upon cell-cell contacts was not observed in FRET imaging. These results indicate that the dislocation of MAGI-1 from the cell-cell contact inhibits Rap1 activation at the cell-cell contacts in motile vascular endothelial cells.
To confirm the requirement of MAGI-1 in Rap1 activation upon vascular endothelial cell-cell contact, we knocked down MAGI-1 in HUVECs using RNA interference. MAGI-1 was almost completely reduced, as examined by Western blotting (Figure 5A). MAGI-1 at the cell-cell junction was not found in the cells treated with siRNA, as examined by immunostaining (Figure 5B). In the same setting, siRNA-introduced HUVECs expressing Raichu-Rap1 were subjected to FRET imaging. Rap1 activation upon cell-cell contact was significantly suppressed in MAGI-1-depleted cells (Figure 5C and Supplementary Movie 5). Quantitative FRET imaging analysis was performed to quantitatively analyze the activation of Rap1 at the cell-cell contacts in MAGI-1-depleted cells (Figure 5D and Supplementary Figure 2). We notice that Rap1 activation was detected at the free ruffled membrane without cell-cell contacts, similarly to control cells (Supplementary Movies 1 and 5), even in the MAGI-1-depleted cells. Collectively, these data suggest that the localization of MAGI-1 to cell-cell contacts through binding to -catenin is involved in Rap1 activation.
Depletion of MAGI-1 Results in Impairment of VE-Cadherin-based Cell Adhesion
To elucidate the role of activated Rap1 downstream of MAGI-1 upon cell-cell contact, we examined the effect of depletion of MAGI-1 on VE-cadherin/-catenin-based cell-cell contact after calcium switch. The localization of VE-cadherin and -catenin at cell-cell contacts in confluent monolayer-cultured HUVECs was unchanged by MAGI-1 siRNA treatment. Because calcium switch induces cadherin-mediated cell junction after its disruption, we looked at the localization of VE-cadherin and -catenin during calcium switch by immunostaining for VE-cadherin and -catenin. VE-cadherin was reaccumulated at cell-cell junctions together with -catenin within 20 min in control HUVECs. In clear contrast, there was a significant impairment of the formation of VE-cadherin/-catenin-based cell junction in MAGI-1-depleted HUVECs (Figure 6).
TJ formation was not affected by MAGI-1 depletion and calcium switch (Figure 7A), whereas the recovery of VE-cadherin-based cell adhesion was substantially impaired in MAGI-1-depleted cells. These results indicate that MAGI-1-mediated signal is important for VE-cadherin/-catenin-based cell adhesion.
We and others have previously reported that Rap1 activation enhances cell adhesions (Fukuhara et al., 2005; Kooistra et al., 2005). Cortical actin formation is enhanced by Rap1 activation and strengthens VE-cadherin-based cell-cell adhesion. Vinculin supports the cortical actin by linking -catenin to -actinin and by directly functioning as an actin-bundling molecule (Kobielak and Fuchs, 2004). Thus we investigated the vinculin localization after calcium switch by immunostaining. Vinculin was observed at the cell-ECM contacts presumably by translocating from cell-cell contacts after calcium depletion. Calcium restoration induced the relocation of vinculin from cell-ECM to cell-cell contact in control siRNA-treated cells. In clear contrast, vinculin remained at the focal adhesions in MAGI-1-depleted cells after calcium switch (Figure 7B). These data suggest that MAGI-1-dependent Rap1 activation at cell-cell contact may affect the vinculin localization, thereby regulating VE-cadherin-based cell adhesion.
MAGI-1 Is Required for VE-Cadherin-mediated Cell Adhesion
Vascular endothelial cell adhesion entails VE-cadherin-based adhesion and other cell adhesion molecules-based cell adhesion. To directly assess the involvement of MAGI-1 in VE-cadherin-mediated cell adhesion, we examined the adhesion of control siRNA-treated HUVECs and MAGI-1-depleted HUVECs onto VEC-Fc-coated dishes. The adhesion was quantified by the ALP activity of cells attaching to the dish after washing. Control HUVECs adhered to the VEC-Fc-coated dish in a time-dependent manner, whereas MAGI-1-depleted HUVECs exhibited significantly impaired adhesion to the VEC-Fc-coated dish (Figure 8A). No cells attached to the Fc-coated dish. MAGI-1-depleted HUVECs adhered to the collagen-coated dish comparably to control HUVECs (Figure 8B). We proceeded to examine the effect of inactivation of Rap1 on VE-cadherin-dependent adhesion. Control adenovirus-infected HUVECs adhered to VEC-Fc-coated dish, whereas Rap1GAPII-expressing adenovirus-infected HUVECs did not (Figure 8C). These results indicate that MAGI-1 and Rap1 activation is required for VE-cadherin-dependent cell adhesion.
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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). Rap1, vice versa, regulates E-cadherin-mediated cell adhesion (Price et al., 2004). In addition to E-cadherin, homophilic dimerization of nectin at the AJs triggers Rap1 activation downstream of Src-Crk (Fukuyama et al., 2005). During the calcium switch experiment, which requires extracellular Ca2+, we found that Rap1 was activated (Figure 1C), indicating extracellular Ca2+-dependent signal, namely cadherin- and nectin-independent signal, appears to be involved in Rap1 activation upon cell-cell contact. In the present study, we propose the involvement of the MAGI-1/PDZ-GEF1 complex in Rap1 activation, besides nectin-mediated Rap1 activating signal and the subsequent positive feedback regulation of VE-cadherin-mediated cell adhesion.
Rap1 is responsible for maintenance and maturation of AJs. The establishment of cadherin-dependent cell-cell contacts is attributable to Rap1 in Drosophila melanogaster and mammalian cells (Knox and Brown, 2002; Price et al., 2004). Consistently, we show here that VE-cadherin-dependent cell adhesion triggers a signal implicating MAGI-1 in Rap1 activation, presumably the MAGI-1-PDZ-GEF1-Rap1 pathway. VE-cadherin engagement-induced Rap1 activation may contribute to AJ formation in addition to homophilic engagement of nectin-dependent Rap1 activation (Fukuyama et al., 2005).
Here we demonstrate VE-cadherin-dependent Rap1 activation besides nectin-dependent Rap1 activation. Calcium switch does not alter localization of nectin at the cell-cell contacts (Yamada et al., 2005). We found that Rap1 was activated after calcium restoration in the calcium switch experiment that mimics nascent cell-cell contact formation (Figure 1), indicating that Ca2+-dependent cell-cell contact is involved in Rap1 activation. We first assumed that VE-cadherin is responsible for Rap1 activation. Indeed, VE-cadherin depletion inhibited cell-cell contact-mediated Rap1 activation (Figure 1F).
These two AJ molecules, VE-cadherin and nectin, are linked by their cytoplasmic domain-associating proteins (Tachibana et al., 2000). L-afadin, a nectin cytoplasmic domain-binding molecule, binds to -catenin and subsequently locates cadherin to AJs without the transinteraction of cadherin (Tanaka et al., 2003). L-afadin, s-afadin (AF-6), and Canoe (Drosophila orthologue of AF-6) contain a Rap1-binding domain (Boettner et al., 2000, 2003). Thus, afadin regulated by activated Rap1 at cell-cell contacts may enhance AJ formation constituted by both cadherin and nectin.
We explored the requirement of MAGI-1 for Rap1 activation at cell adhesion. The association of MAGI-1 with -catenin via the PDZ domain 5 is critical for its localization to VE-cadherin-based cell-cell contact. MAGI-1 also interacts with endothelial cell-selective adhesion molecule (ESAM) and JAM-4 at TJs (Hirabayashi et al., 2003). Other JAM family members (JAM-A, B, and C) do not bind to MAGI-1 (our unpublished data). The carboxy-terminal sequence of ESAM and JAM-4 provides the class I PDZ-binding motif, whereas that of JAM family members contains the class II PDZ-binding motif (Hung and Sheng, 2002). Thus, ESAM-mediated MAGI-1 recruitment may contribute to Rap1-regulated cell adhesion at TJs as -catenin recruits MAGI-1 at AJs. It will be interesting to explore the TJ-dependent Rap1 activation.
MAGI-1 together with MAGI-2 (S-SCAM), and MAGI-3 constitute the MAGI family (Hirao et al., 2000; Franklin et al., 2005). It has been shown that MAGI-2 binds to -catenin and that MAGI-3 colocalized to -catenin in astrocytes expressing E-cadherin (Adamsky et al., 2003; Subauste et al., 2005). Although MAGI-2 is exclusively expressed in the brain, MAGI-3 is ubiquitously expressed. Although we cannot exclude the involvement of MAGI-3 in the activation of Rap1 in vascular endothelial cells, the disconnection of MAGI family members from -catenin by overexpressing the PDZ domain 5 of MAGI-1 perturbed the Rap1 activation upon cell-cell contact (Figure 4, D and E). Furthermore, depletion of MAGI-1 by siRNA hampered the Rap1 activation (Figure 5), suggesting that MAGI-1 is indispensable for Rap1 activation based on the linkage between VE-cadherin--catenin complex and MAGI-PDZ-GEF1 complex in vascular endothelial cells.
To delineate the VE-cadherin engagement-triggered Rap1 activation signal, we tried to test the requirement of PDZ-GEF1 for Rap1 activation because we found that MAGI-1 associated with PDZ-GEF1 in vascular endothelial cells (Figure 3), as this association reported previously (Dobrosotskaya and James, 2000; Kawajiri et al., 2000). PDZ-GEF1-depleted cells seemed to be detached from the collagen-coated dishes and the adhesive activity to collagen-coated dish was significantly inhibited. Thus we assumed that there might be other signaling besides MAGI-1-PDZ-GEF1-mediated signal for cell adhesion and thus concluded that PDZ-GEF1-depleted cells were not appropriate for further evaluation to delineate the signaling. At least, VE-cadherin engagement-triggered Rap1 activation requires MAGI-1.
Activated Rap1 upon cell-cell contact further strengthens the VE-cadherin-dependent cell-cell adhesion. Rap1 activation is required for VE-cadherin-mediated cell adhesion (Figure 8B). We previously reported that the inside-out signal regulated by cAMP-Epac-Rap1 signal enhances the VE-cadherin-mediated cell-cell contacts by regulating cortical actin (Fukuhara et al., 2005). Although we did not observe significant cortical actin distribution after calcium switch experiment (unpublished data), we noticed that the relocation of vinculin from cell-ECM to cell-cell contacts was inhibited in MAGI-1-depleted cells. Because vinculin supports the cadherin-based cell contacts by linking actin to cytoplasmic domain of cadherin through -catenin, inhibition of Rap1 activation by MAGI-1 depletion might affect AJ formation. These results imply a positive feedback loop in cell-cell contact regulated by Rap1; namely, cell-cell contact promotes transdimerization of cell surface adhesion molecules, inducing Rap1 activation followed by further tightening of VE-cadherin-mediated cell adhesion.
In conclusion, we revealed that MAGI-1 is required for Rap1 activation upon cell-cell contacts and in turn for AJ formation. The translocation of MAGI-1 to cell-cell contacts is ascribed to its association with -catenin. The MAGI-1-associating molecule, PDZ-GEF1, may account for Rap1 activation.
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ACKNOWLEDGMENTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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Footnotes |
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Abbreviations used: AJ, adherens junction; CFP, cyan fluorescent protein; ECM, extracellular matrix; EGFP, enhanced green fluorescent protein; FRET, fluorescence resonance energy transfer; GEF, guanine nucleotide exchange factor; GAP, GTPase activating protein; HAEC, human aortic endothelial cell; HUVEC, human umbilical vascular endothelial cell; JAM, junctional adhesion molecule; MAGI-1, MAGUK with inverted domain structure-1; GFP, green fluorescent protein; PBS, phosphate-buffered saline; PDZ, PSD95/DiscLarge/ZO-1; PECAM-1, platelet and endothelial cell adhesion molecule-1; siRNAs, small interfering RNAs; TJ, tight junction; VE-cadherin, vascular endothelial cadherin; VEC-Fc, recombinant VE-cadherin ectodomain-Fc chimera; YFP, yellow fluorescent protein.
The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org). 
Address correspondence to: Naoki Mochizuki (nmochizu{at}ri.ncvc.go.jp).
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REFERENCES |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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