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Vol. 17, Issue 4, 1834-1844, April 2006
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Division of Cellular Biochemistry and Center for Biomedical Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
Submitted July 28, 2005;
Revised January 18, 2006;
Accepted January 24, 2006
Monitoring Editor: Carl-Henrik Heldin
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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. Inactivation or knockdown of Pyk2 inhibits LPA-induced (but not basal) tyrosine phosphorylation of GSK-3 and partially inhibits LPA-induced neurite retraction, similar to what is observed following GSK-3 inhibition. Thus, Pyk2 mediates LPA1-induced activation of GSK-3 and subsequent phosphorylation of microtubule-associated proteins. Pyk2-mediated GSK-3 activation is initiated by PIP2 hydrolysis and may serve to destabilize microtubules during actomyosin-driven neurite retraction. |
INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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; Doble and Woodgett, 2003; Jope and Johnson, 2004). Mammalian GSK-3 exists as two isoforms encoded by distinct genes, GSK-3
(51 kDa) and GSK-3 (47 kDa; Woodgett, 1990), with two splice variants of GSK-3 (Mukai et al., 2002; Schaffer et al., 2003). Highest expression of GSK-3 is observed in developing brain, where its expression correlates with the period of active neurite remodeling (Woodgett, 1990; Takahashi et al., 1994; Leroy and Brion, 1999). In accordance with this, studies on cultured neuronal cells point to an important role of GSK-3 in regulating neurite morphology (Eickholt et al., 2002; Sayas et al., 2002a; Zhou et al., 2004; Yoshimura et al., 2005; Jiang et al., 2005): inactivation and activation of GSK-3 promote neurite outgrowth and withdrawal, respectively, the key processes in nervous system development and plasticity. The ability of GSK-3 to regulate neuronal architecture is thought to rely on its ability to phosphorylate microtubule-binding proteins, particularly the neuron-specific proteins tau (Hanger et al., 1992; Wagner et al., 1996), MAP1B (Trivedi et al., 2005) and CRMP-2 (Yoshimura et al., 2005), and the widely expressed adenomatous polyposis coli protein (APC; Zhou et al., 2004). Phosphorylation of these proteins by GSK-3 promotes microtubule disassembly and destabilization, which inhibits or repels axon growth (Zhou and Snider, 2005). Importantly, hyperphosphorylated tau is a major component of the neurofibrillary tangles detected in certain neurodegenerative diseases including Alzheimer's disease (Grimes and Jope, 2001; Drewes, 2004), which provides a strong rationale for developing selective GSK-3 inhibitors for the treatment of such neuropathologies (Cohen and Goedert, 2004).
The activity of GSK-3 is controlled by phosphorylation and interaction with inhibitory proteins. Unlike other protein kinases, GSK-3 is constitutively active under resting conditions and is inactivated by extracellular signals through phosphorylation of an N-terminal serine residue, Ser-9 in GSK-3 and Ser-21 in GSK-3. Various kinases have been implicated in mediating serine phosphorylation and inactivation of GSK-3, including PI3-kinase (PI3K)-regulated Akt/PKB, protein kinase A and protein kinase C (PKC; Cross et al., 1995; Fang et al., 2000, 2002). GSK-3 also plays a central role in the canonical Wnt pathway, in which the enzyme is displaced from a multiprotein complex and thereby unable to phosphorylate its substrates such as -catenin (Doble and Woodgett, 2003).
In opposition to inhibitory serine phosphorylation, GSK-3 activity is increased by phosphorylation of a tyrosine residue, Tyr-216 in GSK-3 and Tyr-279 in GSK-3, located in the kinase domain. This phosphotyrosine is important for activity because its dephosphorylation diminishes activity (Hughes et al., 1993; Wang et al., 1994), but the mechanism responsible for tyrosine phosphorylation of GSK-3 remains unclear. In Dictyostelium discoideum, the tyrosine kinase ZAK1 mediates GSK-3 phosphorylation and activation in response to cAMP (Kim et al., 1999). In mammalian cells, the tyrosine kinases Fyn, Pyk2, and Csk have been implicated in phosphorylating GSK-3 (Lesort et al., 1999; Hartigan et al., 2001; Fan et al., 2003), but some of these claims have been questioned (Cole et al., 2004).
In neuronal cells, GSK-3 is tyrosine phosphorylated and activated during neurite retraction induced by the serum-borne lipid mediator lysophosphatidic acid (LPA; Sayas et al., 1999, 2002b), but how LPA activates GSK-3 is unclear. LPA acts on at least four distinct G protein-coupled receptors (GPCRs), termed LPA1-4 (Chun et al., 2002; Noguchi et al., 2003), that signal via multiple G proteins, including Gq/11, Gi/o, and G12/13, to induce a great diversity of cellular responses (Moolenaar et al., 2004). LPA-induced neurite retraction is primarily driven by actomyosin-based contractile forces initiated by G12/13-linked activation of RhoA and its downstream effector Rho-kinase (ROCK; Jalink et al., 1994; Hirose et al., 1998; Kranenburg et al., 1999). Activated GSK-3 may contribute to optimal neurite retraction by phosphorylating microtubule-binding proteins leading to microtubule destabilization. In the present study, we set out to identify the G protein-effector pathway and the tyrosine kinase that mediates phosphorylation and activation of GSK-3 in neuronal cells after stimulation of the prototypic LPA1 receptor. We show that GSK-3 is tyrosine phosphorylated by the Ca2+-sensitive tyrosine kinase Pyk2 as a direct consequence of phospholipase C activation.
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MATERIALS AND METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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). Neurite outgrowth was induced by exposing the cells to serum-free medium for >18 h or, in case of SH-SY5Y cells, Neurobasal medium containing B-27 supplement and 1 mM db-cAMP for 72 h (Sayas et al., 1999). Chemicals and reagents were obtained from the following sources: 1-oleoyl-LPA, Sigma (Zwijndrecht, The Netherlands); BAPTA-AM, tyrphostin A9, PP2, and AG-1296, Calbiochem (La Jolla, CA); SB-216763 and SB-415286, Tocris (Ballwin, MO); pertussis toxin, List Biological Laboratories (Campbell, CA). The following antibodies were used: anti-GSK-3/ monoclonal antibody (mAb) and rabbit anti-phospho-GSK-3/ (pTyr279/216), Biosource (Camarillo, CA) and Sigma; rabbit phospho-GSK-3/ (pSer21/9), Cell Signaling (Beverly, MA); anti-GSK-3 mAb, BD Transduction Laboratories (Lexington, KY). Monoclonal 7.51 against total tau was a gift from J. Avila (Centro de Biologia Molecular, Madrid, Spain). Antibody PHF-1 against phosphorylated tau (Ser396/404) was supplied by P. Davies (Albert Einstein College, New York). Rabbit anti-pTyr402-Pyk2 was obtained from Biosource and mAb against total Pyk2 was from BD Transduction Laboratories. Monoclonal anti-phosphotyrosine antibody 4G10 was from Upstate Biotechnology (Lake Placid, NY), monoclonal anti-Flag M2 from Sigma and polyclonal anti-GFP antibody from J. Neefjes (NKI, Amsterdam, The Netherlands).
Cell Morphology
Serum-starved cells in 35-mm dishes were placed in a heat chamber (37°C) with a constant CO2 infusion. Phase-contrast and corresponding fluorescence images were taken on a Zeiss inverted microscope (Thornwood, NY) equipped with a CCD camera. Neurites were defined as processes longer than one cell body diameter.
Immunoblotting
Total cell lysates were prepared by washing the cells with phosphate-buffered saline (PBS) followed by resuspension in lysis buffer containing 1% SDS, 1 mM EDTA, 1 mM EGTA, and 25 mM Tris (pH 7.5). Lysates were boiled for 10 min., spun down, and sonicated for 15 s. Proteins (25-50 µg) were separated by SDS-PAGE and transferred to nitrocellulose filters. The filters were blocked with 10% nonfat milk powder or 5% BSA in Tris-buffered saline (TBS)-0.1% Tween 20 (TBS-T), and subsequently incubated with primary antibodies overnight (4°C) followed by incubation with the corresponding peroxidase-conjugated secondary antibody (DAKO, Carpinteria, CA) for 1 h. Immunoreactivity was visualized by enhanced chemiluminescence detection (Amersham). Densitometric analysis was performed using TINA 2.0 software.
GSK-3 In Vitro Kinase Assay
To assay GSK-3 activity in cell extracts, control and LPA-treated B103-LPA1 cells were lysed in a buffer containing 100 mM NaCl, 100 mM NaF, 1 mM sodium orthovanadate, 5 mM EDTA, 100 nM okadaic acid, 20 mM HEPES (pH 7.4), 1% Triton X-100 and protease inhibitors. Lysates were centrifuged at 14000 rpm for 15 min at 4°C to obtain the soluble fraction, which was used as a source of GSK-3 activity. Soluble cell extracts (7 µg) were incubated in a buffer containing 25 mM Tris (pH 7.5), 1 mM DTT, 10 mM MgCl2, and the GSK-3-specific eIF2B-based substrate peptide 2B-SP (0.75 mg/ml; Welsh et al., 1997) in the presence of [
-32P]ATP. The reaction was stopped after 1 h by spotting aliquots on P81 phosphocellulose paper followed by scintillation counting. Assays were performed in the presence or absence of LiCl (20 mM; Sayas et al., 1999). The difference between the kinase activity in the presence or absence of LiCl was considered a measure of GSK-3 activity. Activity values were normalized with respect to GSK-3 expression levels.
Transfection and Immunoprecipitation
B103-LPA1 and Neuro2A cells were transfected using Lipofectamine Plus reagents (Invitrogen, Carlsbad, CA) or Fugene (Roche), respectively. The following constructs were used: GFP-tagged versions of human wild-type Pyk2 and kinase-dead Pyk2 (Pyk2 K457A; provided by Drs. Sancho and Sanchez-Madrid, Hospital de la Princesa, Madrid, Spain); HA-tagged human GSK-3 (a gift from J. Woodgett, The Ontario Cancer Institute, Toronto, Canada); Myc-tagged mouse GSK-3 and kinase-inactive GSK-3(R85) (KI-GSK-3-Myc; from F. Wandosell, Centro de Biologia Molecular "Severo Ochoa," Madrid, Spain) and Myc-tagged rat Pyk2 (a gift from H. Earp, University of North Carolina at Chapel Hill, North Carolina). Because B103 transfection efficiency is low, we cotransfected a plasmid bearing puromycin resistance. Transfected cells were maintained in the presence of puromycin (1 µg/ml) for 30-48 h to enrich the population of transfected cells. Human SH-SY5Y neuroblastoma cells were transfected with a GFP-tagged human Pyk2 construct (gift from Drs. Sancho and Sanchez Madrid, Hospital de La Princesa) or pEGFP (Clontech), using Lipofectamine Plus reagents (Invitrogen). Cells were then selected for 2 wk in the presence of G-418 (GIBCO, Invtrogen Cell Culture). Selected cells were grown for 2 more weeks to obtain stable SH-SY5Y-GFP and SH-SY5Y-Pyk2-GFP cell lines. SH-SY5Y cells were differentiated as described (Sayas et al., 1999). For immunoprecipitation, cells were washed twice with PBS and lysed in a buffer containing 50 mM Tris (pH 7.5), 1 mM EDTA, 150 mM NaCl, 10 mM NaF, 1% NP-40, 2 mM sodium orthovanadate, and protease inhibitors. Lysates were centrifuged at 14,000 rpm, precleared, and incubated with the appropriate primary antibodies overnight at 4°C. Thereafter, protein A or G was added to the lysates and incubated at 4°C for 1 h. Immunoprecipitates were analyzed by SDS-PAGE and immunoblotting.
Immunofluorescence and Quantification of Cell Contraction
B103-LPA1 cells were cotransfected with either pcDNA3-Myc and GFP or KI-GSK-3-Myc and GFP in a 3:1 ratio. Cells were treated with LPA for 15-30 min or left untreated. After treatments, cell cultures were fixed with PBS containing 4% (wt/vol) paraformaldehyde for 20 min. After several washes with PBS, the cells were preincubated in PBS, containing 0.1% Triton X-100 and 3% BSA for 30 min. Cells were then incubated overnight at 4°C with mAb anti-Flag M2. After washing, cultures were incubated with the appropriate secondary antibody, conjugated to Alexa-568 (Molecular Probes, Eugene, OR) for 45 min. After washing, coverslips were mounted with Vectashield (Vector Laboratories, Burlingame, CA). Cells were visualized with a Leica fluorescence microscope. Green cells with and without neurites were counted in each case. Pictures were taken with a CCD camera.
Pyk2 RNA Interference
Four different Pyk2 small interfering RNA (siRNA)-targeting vectors (pS-GFP-Pyk2) were based on four 19-mer sequences present in the coding sequence of rat Pyk2: 1) 5'-tgcacagtgcagacagaga-3'; 2) 5'-ctcattcaagggtggaaca-3'; 3) 5'-gatgtagttcttaaccgca-3'; 4) 5'-gatgcttggacccgatggt-3'. Blast search revealed no off-sequence targeting. 64-mer synthetic oligonucleotides for cloning into pSuperGFP (pS-GFP) were synthesized, annealed, and ligated into pS-GFP, which includes GFP under control of the pGK promoter (Brummelkamp et al., 2002; Mulder et al., 2004). Thus, detection of cells expressing the siRNAs was possible by searching for GFP-positive cells. Of the four different targeting constructs tested, pS-GFP-Pyk2-3 and pS-GFP-Pyk2-4 were most efficient in down-regulating Pyk2 mRNA. Adenoviral pAS constructs were designed as follows: pS-GFP-Pyk2-3 or pS-GFP were digested and the insert containing the RNAi targeting sequence and promotor was ligated into pENTR1A of the virapower adenoviral expression system (Invitrogen).
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1 fused to cyan and yellow fluorescent proteins (CFP and YFP), as described previously (van der Wal et al., 2001). PH-CFP and PH-YFP chimeric constructs were transiently transfected into B103-LPA1 cells at a 1:1 M ratio. After 24 h, cells were transferred to an inverted epifluorescence microscope and assayed for FRET by simultaneously monitoring the emission of CFP (475 nm) and YFP (530 nm), while exciting CFP at 425 nm. After hydrolysis of PIP2, the PH domains rapidly dissociate from the plasma membrane and the FRET signal decreases (van der Wal et al., 2001). Ca2+ mobilization was measured by loading the cells with the Ca2+-sensitive fluorescent dye Indo-1/AM (Molecular Probes) and recording fluorescence intensity (excitation 355 nm, emission 405 nm), as described previously (Jalink et al., 1995). |
RESULTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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). We therefore used B103-LPA1 neuronal cells, which stably express LPA1 at relatively low levels (Van Leeuwen et al., 2003). Removal of serum from these cells results in the formation of neurites, which rapidly retract in response to LPA and other GPCR agonists such as sphingosine 1-phosphate (S1P) and thrombin (Van Leeuwen et al., 2003 and unpublished data).
GSK-3 phosphorylation was examined using phospho-specific antibodies, notably anti-phosphotyrosine-GSK-3(/) (pTyr279/216) and anti-phosphoserine-GSK-3(/) (pSer21/9). Monoclonal anti-GSK-3(/) was used to detect total GSK-3. Treatment of B103-LPA1 cells with 1-oleoyl-LPA (5 µM) induced a rapid, transient increase in tyrosine phosphorylation of GSK-3 above basal levels, reaching a maximum at 2-5 min and accompanying cytoskeletal contraction (Figure 1A, top panel; see also Figure 9A). As expected, LPA receptor-deficient B103 cells failed to respond to LPA (unpublished data). Similar to LPA, other "collapsing" agonists such as S1P and thrombin receptor-activating peptide (TRP) also induced GSK-3 tyrosine phosphorylation (Figure 1B).
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rather than in GSK-3, at least with the antibodies used (from two distinct commercial sources): densitometric analysis showed an 
1.8-fold increase in GSK-3 phosphotyrosine (Figure 1A, bottom panels; a minor increase in GSK-3, detected in some experiments, was barely significant). For convenience, we combined the - and -isoform phosphotyrosine levels in our further analysis of GSK-3. As can be seen in Figure 1, A-C, a third band running below GSK-3 was consistently recognized by anti-phosphotyrosine-GSK-3 antibody; its appearance closely correlated with the increase in tyrosine phosphorylation of GSK-3. Treatment of the cells with the tyrosine phosphatase inhibitor pervanadate markedly enhanced the intensity of all three bands (Figure 1C), indicating that the third band, similar to GSK-3/, is a target for tyrosine kinases. Prolonged exposure of the blots probed with antibodies against total GSK-3/ likewise revealed an additional band running just below GSK-3 (unpublished data). We presume this band represents the GSK-3 splice variant that lacks exon 10 (encoding 33 residues; Schaffer et al., 2003), as it differs from "nonspliced" GSK-3 by the predicted molecular size.
LPA also induced serine phosphorylation of GSK-3 (again, particularly the isoform), with kinetics similar to those of tyrosine phophorylation (Figure 1B). Of note, serine phosphorylation of GSK-3 induced by LPA was weaker than that induced by insulin (Figure 1D), the classic agonist that phosphorylates GSK-3 via the PI3K-Akt/PKB pathway to inhibit its activity (Cross et al., 1995). Pharmacological inhibitor studies suggested that LPA-induced serine phosphorylation of GSK-3 involves the wortmannin-sensitive PI3K-Akt/PKB pathway as well as PKC (unpublished data), but we did not examine the responsible signaling pathways in further detail. The use of pharmacological PI3K and PKC inhibitors allowed an independent confirmation that the anti-GSK-3 phosphotyrosine antibodies were specific in that they did not recognize serine-phosphorylated GSK-3 (unpublished data). Taken together, these results indicate that LPA1 receptor stimulation leads to GSK-3 phosphorylation on both tyrosine and serine residues.
GSK-3 Activation and Tau Phosphorylation
Serine phosphorylation of GSK-3 inhibits its catalytic activity, whereas tyrosine phosphorylation has the opposite effect (Wang et al., 1994; Doble and Woodgett, 2003). To determine how dual Tyr/Ser phosphorylation of GSK-3 affects its enzymatic activity, we performed in vitro kinase assays using a GSK-3-specific substrate peptide. As shown in Figure 2A, LPA induces a net increase in GSK-3 activity, which reaches a maximum at 2 min and returns to basal levels after 10 min of LPA addition (Figure 2A). Thus, the net effect of dual Tyr/Ser phosphorylation of GSK-3 by LPA1 is a transient increase in activity. Our further experiments focused on GSK-3 tyrosine phosphorylation and activation, as serine phosphorylation was beyond the scope of the present study.
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). We examined the phosphorylation state of tau using phospho-specific antibody PHF-1 (anti-pSer396/404 tau) together with monoclonal 7.51 against total tau. Consistent with tau being a downstream target of GSK-3, LPA induced a transient increase in tau phosphorylation (Figure 2B), which was completely inhibited by the GSK-3 inhibitor SB-415286 (40 µM) and two other structurally unrelated inhibitors, SB-216763 (25 µM) and LiCl (20 mM; Cohen and Goedert, 2004; Figure 2C and unpublished data).
GSK-3 Tyrosine Phosphorylation via Gi-mediated PIP2 Hydrolysis and Ca2+ Mobilization
We next explored the LPA1-linked signaling pathway that leads to GSK-3 tyrosine phosphorylation in B103-LPA1 cells. Because intracellular Ca2+ has been implicated in GSK-3 activation (Hartigan and Johnson, 1999) and LPA is a potent Ca2+-mobilizing agonist for numerous cell types (Jalink et al., 1990), we analyzed phospholipase C (PLC) activation following LPA1 stimulation, using thrombin receptor-activating peptide (TRP) as a control. The levels of phosphatidylinositol 4,5-bisphosphate (PIP2) were monitored in real-time using FRET between the PH domains of PLC-1 fused to CFP and YFP, respectively (van der Wal et al., 2001). Figure 3A illustrates that LPA and TRP induced a rapid, transient decrease in PIP2 levels accompanied by a rise in cytosolic free Ca2+ (Figure 3B). Pretreatment of the cells with pertussis toxin (PTX), a selective inhibitor of Gi, largely abrogated LPA-induced PIP2 hydrolysis, Ca2+ mobilization and GSK-3 tyrosine phosphorylation, without affecting the responses to TRP (Figure 3C). Chelation of intracellular free Ca2+ using cell-permeable BAPTA-AM blocked LPA-induced GSK-3 tyrosine phosphorylation, but not basal tyrosine phosphorylation. Conversely, the Ca2+ ionophore ionomycin mimicked LPA in stimulating GSK-3 tyrosine phosphorylation (Figure 3D). From these results we conclude that tyrosine phosphorylation of GSK-3 is mediated by the PLC-Ca2+ mobilization pathway.
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; Dikic et al., 1996; Yu et al., 1996; Avraham et al., 2000; Park et al., 2000). Hence Pyk2 is a strong candidate for mediating tyrosine phosphorylation of GSK-3. Pyk2 is highly expressed in brain and neuronal cell lines (Menegon et al., 1999) and has been implicated in synaptic plasticity and neurite outgrowth (Ivankovic-Dikic et al., 2000; Huang et al., 2001). Activation of Pyk2 by GPCR agonists, including LPA, has previously been documented (Dikic et al., 1996; Andreev et al., 2001; Wu et al., 2002); moreover, it has been reported that Pyk2 can phosphorylate GSK-3 in vitro and in an CHO cell overexpression system (Hartigan et al., 2001).
Among various tyrosine kinase inhibitors, we found tyrphostin A9 to block the LPA-induced tyrosine phosphorylation of GSK-3 (but not basal tyrosine phosphorylation) in B103-LPA1 cells. Tyrphostin A9 is an inhibitor of Pyk2 signaling (Fuortes et al., 1999; Loeser et al., 2003), although it was originally identified as an inhibitor of the platelet-derived growth factor (PDGF) receptor tyrosine kinase (Levitzki and Gilon, 1991). Other tyrosine kinase inhibitors had no effect, including AG1296 (specific for the PDGF receptor), PP2 (selective for Src family members) and STI571 (Gleevec), an inhibitor of c-Abl and modulator of neurite morphology (Woodring et al., 2002; Figure 4A). The inhibitory effect of tyrphositin A9 was not unique for LPA as it was also observed with TRP and S1P (Figure 4B).
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; Dikic et al., 1996). Using Tyr-402 phosphorylation as a readout, we found that LPA rapidly activates Pyk2 in B103-LPA1 cells (Figure 5A), which was confirmed by immunoprecipitation using anti-phosphotyrosine antibody followed by Western blotting using anti-Pyk2 antibody (Figure 5B). LPA-induced Pyk2 activity was blocked by PTX and BAPTA-AM (Figure 5, C and D), consistent with LPA1 mediating Pyk2 activation through a Gi-mediated rise in cytosolic Ca2+.
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(Figure 6A). The reciprocal precipitation yielded inconsistent results, however. We therefore turned to Neuro2A cells, in which the presence of GSK-3 in Pyk2 precipitates (and vice versa) was more readily detectable (Figure 6B). The interaction between Pyk2 and GSK-3 did not require Pyk2 activity, as kinase-dead Pyk2(K457A) also interacted with GSK-3 (Figure 6A).
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To further substantiate the involvement of Pyk2 in LPA1-mediated GSK-3 tyrosine phosphorylation, we used "dominant-negative" Pyk2(K457A) and siRNAs against Pyk2. As shown in Figure 7A, transient introduction of Pyk2(K457A) into B103-LPA1 cells blocked GSK-3 tyrosine phosphorylation in response to LPA. Knockdown of Pyk2 expression was achieved by using the expression vector pSUPER (pS) that directs stable expression of siRNAs (Brummelkamp et al., 2002). Four different GFP-containing constructs were created to target rat Pyk2 mRNA (with GFP expressed from a distinct PGK promotor), termed pS-GFP-Pyk2. Targeting efficacy was tested in HEK293 cells by cotransfecting Pyk2-Myc and pS-GFP-Pyk2. Constructs pS-GFP-Pyk2-3 and pS-GFP-Pyk2-4 were found to knockdown Pyk2 expression by >90% (4 d after transfection; unpublished data). Because knockdown of endogenous Pyk2 expression was 50% in B103-LPA1 cells, these constructs were cotransfected with Myc-tagged Pyk2 to examine the effect on GSK-3 tyrosine phosphorylation. As shown in Figure 7B, both targeting constructs inhibited LPA1-mediated tyrosine phosphorylation of GSK-3 in B103-LPA1 cells.
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), whereas PC12 cells show high expression levels (Figure 8A). In the latter cells, LPA induces GSK-3 tyrosine phosphorylation concurrent with Pyk2 activation (Figure 8B), although the response is relatively weak. Knockdown of endogenous Pyk2, using an adenoviral construct expressing RNAi3, abolished LPA-induced GSK-3 tyrosine phosphorylation in PC12 cells (and partially inhibited basal GSK-3 tyrosine phosphorylation; Figure 8C). In Pyk2-deficient SH-SY5Y cells, LPA failed to increase GSK-3 tyrosine phosphorylation above basal levels (Figure 8D); but after forced expression of Pyk2, LPA-induced GSK-3 tyrosine phosphorylation was readily detectable (Figure 8E). Thus, Pyk2 mediates LPA-induced tyrosine phosphorylation of GSK-3 in different neuronal cell types.
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Inhibition of Pyk2 and GSK-3 Interferes with LPA1-mediated Neurite Retraction
Because GSK-3 activation by LPA coincides with neurite retraction, we examined whether the Pyk2-GSK-3 pathway is involved in neurite remodeling in B103-LPA1 cells. Three different pharmacological inhibitors of GSK-3 (lithium, SB-415286 and SB-216763) suppressed LPA1-mediated neurite retraction and cell rounding (Figure 9, A and B). The number of contracted cells (at 15 min after LPA addition) dropped from 65% in untreated cells to 35% in cells treated with the GSK-3 inhibitors (Figure 9A). In addition, expression of "dominant-negative" (kinase-inactive [KI]) GSK-3 also interfered with LPA-induced contraction (Figure 9C). Thus, GSK-3 activity is required for optimal neurite retraction.
Similar to what is observed with the GSK-3 inhibitors, the Pyk2 inhibitor tyrphostin A9 partially inhibited LPA-induced neurite retraction (Figure 10A, left panel). About 90% of the control cells underwent neurite retraction within 30 min of LPA addition, whereas only 47% of the A9-pretreated cells showed a contractile response (Figure 10A, right panel). Knockdown of Pyk2 mRNA in B103-LPA1 cells, using construct pS-GFP-Pyk2-4, induced the formation of neuritelike extensions that were partially resistant to LPA (Figure 10B). Together, these findings indicate that the Pyk2-GSK-3 pathway is required for optimal neurite retraction in response to LPA.
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSREFERENCES |
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, 2002b), a potent inducer of G12/13-RhoA-mediated growth cone collapse and neurite retraction. However, the signaling events and the tyrosine kinase(s) responsible for GSK-3 activation by LPA have remained unclear.
Growth cone collapse and neurite retraction in response to repulsive cues are essential to achieve correct neural connectivity during nervous system development; furthermore, neurite retraction occurs during neurodegeneration and could also be important during traumatic brain injury, when neurons are suddenly exposed to blood-borne factors such as LPA. In the present study, we have examined how LPA1, the predominant LPA receptor in the nervous system, induces stimulatory tyrosine phosphorylation of GSK-3, a response leading to phosphorylation of tau and accompanying neurite retraction. LPA1 signaling has been implicated in neurogenesis and suppression of apoptosis during brain development (Fukushima et al., 2001; Kingsbury et al., 2003), while it leads to neurite retraction and cell migration in cultured neuronal cells (Van Leeuwen et al., 2003). We show here that LPA1 stimulates tyrosine phosphorylation of GSK-3 through the Ca2+-sensitive tyrosine kinase Pyk2 downstream of the Gi-linked PLC-Ca2+ mobilization pathway. Our conclusion is based on the following findings: 1) LPA-induced tyrosine phosphorylation of GSK-3 is inhibited by chelation of intracellular Ca2+ and mimicked by the Ca2+ ionophore ionomycin; 2) LPA activates Pyk2 in a pertussis toxin- and Ca2+-dependent manner and with kinetics similar to those of GSK-3; moreover, Pyk2 and GSK-3 exist in a complex as both enzymes can be coimmunoprecipitated from cell lysates; 3) pharmacological inhibition, kinase-dead Pyk2 as well as siRNAs against Pyk2 interfere with LPA1-mediated tyrosine phosphorylation of GSK-3; and 4) LPA fails to increase GSK-3 tyrosine phosphorylation in Pyk2-deficient SH-SY5Y neuroblastoma cells, whereas the response is restored after forced expression of Pyk2; yet LPA does activate GSK-3 in SH-SY5Y cells (Sayas et al., 1999), suggesting the existence of an alternative mechanism of GSK-3 activation, presumably serine dephosphorylation as is observed with the axonal repellent Sema3A (Eickholt et al., 2002). Other collapsing factors, notably thrombin and S1P, provoke the same signaling events as LPA, suggesting that the Pyk2-GSK-3 pathway is a general component of the neurite retraction response. Thus, different repulsive/collapsing agonists converge at GSK-3 to increase its activity through tyrosine phosphorylation or/and serine dephosphorylation.
We find that pharmacological inhibition or knockdown of Pyk2 partially inhibits LPA1-mediated neurite retraction in B103-LPA1 cells, as is also observed with three structurally unrelated inhibitors of GSK-3 and a dominant-negative (kinase-dead) version of GSK-3, suggesting that the Pyk2-GSK-3 pathway is necessary for full neurite retraction. Previous studies on PC12 cells have implicated Pyk2 in the regulation of neurite outgrowth following costimulation of growth factor receptors and integrins (Ivankovic-Dikic et al., 2000; Haglund et al., 2004), whereas another study has suggested involvement of Pyk2 in neurite retraction (Park et al., 2000). Supporting evidence for a role for Pyk2 in cytoskeletal contraction comes from Pyk2 knockout studies showing that Pyk2 is essential for lamellipodial contractile activity in macrophages (Okigaki et al., 2003). It thus seems that the activity of Pyk2 may increase during retraction as well as outgrowth of developing neurites, likely depending on its subcellular localization and specific binding partners.
Precisely how Pyk2-activated GSK-3 participates in neurite retraction remains to be clarified, but it seems likely that GSK-3 primarily acts by promoting microtubule dynamics through its ability to phosphorylate microtubule-binding proteins implicated in axon growth, notably tau, CRMP-2, APC, and MAP1B (Zhou and Snider, 2005). Microtubules constitute the main cytoskeletal component in the shaft of developing neurites and should disassemble during neurite retraction. We propose that the Pyk2-GSK-3-microtubule destabilization pathway functions in parallel and cooperates with the force-generating RhoA-ROCK-actomyosin pathway to induce optimal neurite retraction, as schematically illustrated in Figure 11.
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; Cole et al., 2004). It has been argued that basal tyrosine phosphorylation of GSK-3 represents an intramolecular autophosphorylation event (Cole et al., 2004) but, as we show here, agonist-induced tyrosine phosphorylation is not. Pharmacological inhibition of GSK-3, using either LiCl or SB-216763, did not suppress LPA-induced GSK-3 tyrosine phosphorylation (unpublished data), indicating that basal kinase activity is not required for GSK-3 to become tyrosine phosphorylated by LPA. Furthermore, we find that tyrosine kinase inhibitors interfere with agonist-induced GSK-3 tyrosine phosphorylation, but not with basal tyrosine phosphorylation. In other words, agonist-induced tyrosine phosphorylation of GSK-3 can be dissociated from its basal tyrosine phosphorylation. (Yet in PC12 cells, Pyk2 knockdown did reduce basal GSK-3 phosphotyrosine levels). Whether basal tyrosine phosphorylation of GSK-3 represents primarily an autocatalytic event or is mediated by another kinase remains to be established.
In a previous study, using activated G subunits, it was reported that GSK-3 activation in neuronal cells occurs downstream of G12/13 through Rho-dependent and -independent pathways (Sayas et al., 2002a); however, possible involvement of the PLC-Ca2+ mobilization pathway was not examined. Yet it remains possible that LPA can bypass the PLC pathway to activate Pyk2/GSK-3 via enhanced Ca2+ influx (as opposed to Ca2+ mobilization from internal stores). For example, LPA evokes a prominent G13-mediated membrane depolarization (Postma et al., 2001), which in turn could stimulate voltage-dependent Ca2+ entry and subsequent activation of Pyk2/GSK-3.
We find that LPA phosphorylates GSK-3(/) not only on tyrosine but also on serine (Ser-21 and Ser-9, respectively). But compared with the strong and sustained phosphorylation induced by insulin, LPA is relatively weak in stimulating GSK-3 serine phosphorylation in B103-LPA1 cells. Probably only a small proportion of GSK-3 molecules is serine phosphorylated by LPA. Thus, the net result of LPA1-mediated dual tyrosine/serine phosphorylation of GSK-3 is an increase in catalytic activity above basal levels. Interestingly, treatment of primary neurons with a neurotoxic prion peptide also induces dual serine/tyrosine phosphorylation of GSK-3 with a net increase in activity (Perez et al., 2003). By contrast, in Swiss 3T3 cells LPA is a relatively strong inducer of GSK-3 serine phosphorylation leading to inactivation of the enzyme (Fang et al., 2002). Reconstitution experiments in HEK293 cells suggest that GSK-3 serine phosphorylation is mediated by LPA2 and LPA3, but not LPA1 (Fang et al., 2002). Although the authors did not analyze the tyrosine phosphorylation state of GSK-3, the discrepancy with the present results is most likely due to differences in cell type (neuronal vs. nonneuronal), LPA receptor expression profile and the relative strength of the corresponding intracellular signals.
In conclusion, we have defined a PLC-Ca2+-Pyk2 pathway that couples LPA1 to GSK-3 tyrosine phosphorylation and activation in neuronal cells. As one would predict, other collapsing (i.e., RhoA-activating) and PIP2-hydrolyzing GPCR agonists, notably thrombin and S1P, also induce GSK-3 tyrosine phosphorylation concomitant with neurite retraction. It thus appears that transient tyrosine phosphorylation and activation of GSK-3, with subsequent phosphorylation of microtubule-associated proteins, is one of the initial steps during neurite retraction induced by GPCRs that signal via both RhoA and PLC. Both Pyk2 and GSK-3 are highly expressed in the developing nervous system as well as adult brain, where they are thought to play important roles in synapse formation and plasticity (Henley and Nishimune, 2001; Tokuoka et al., 2002). In cultured neuronal cells, GSK-3 and Pyk2 appear to be enriched in neurites and growth cones (Menegon et al., 1999; Zhou et al., 2004). Pyk2 may regulate neuronal GSK-3 activity in vivo, because ischemic brain injury leads to Pyk2 activation (Tian et al., 2000) as well as GSK-3 tyrosine phosphorylation (Bhat et al., 2000), consistent with Pyk2 phosphorylating GSK-3 under pathological conditions. It will be of interest to examine whether Pyk2 activity in brain is deregulated in Alzheimer patients or after brain injury and thereby may lead to undue activation of GSK-3.
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Abbreviations used: GSK-3, glycogen synthase kinase-3; LPA, lysophosphatidic acid; Pyk2, proline-rich tyrosine kinase 2.
* Present address: Department of Cell Biology and Genetics, Erasmus University, 3000 DR Rotterdam, The Netherlands. 
Address correspondence to: C. Laura Sayas (c.sayascasanova{at}erasmusmc.nl).
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REFERENCES |
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6; error bars, SD). (B) Immunoblot analysis of total GSK-3 tyrosine phosphorylation in cells treated for 5 min with either LPA (5 µM), thrombin receptor-activating peptide (TRP, 100 µM) or S1P (200 nM). (C) Cells were treated with sodium pervanadate (PV) for 5 min and GSK-3 phosphotyrosine was analyzed by immunoblotting as in A. (D) Immunoblot analysis of GSK-3
