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Vol. 17, Issue 6, 2592-2603, June 2006
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Department of Molecular Immunology, Institute of Development, Aging, and Cancer, Tohoku University, Sendai 980-8575, Japan
Submitted October 3, 2005;
Revised March 20, 2006;
Accepted March 20, 2006
Monitoring Editor: Suzanne Pfeffer
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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; Lewin and Mellman, 1998; Scales et al., 2000). Thereafter, monomeric adaptor proteins such as AP180 and CALM induce clathrin assembly, thereby generating clathrin-coated vesicles (Hao et al., 1999; Tebar et al., 1999; Ford et al., 2001). Subsequently, the budded vesicles fuse to the acceptor membrane where Hsc70 and auxilin disassemble the clathrin coat (Cremona, 2001; Lemmon, 2001; Morgan et al., 2001; Fotin et al., 2004).
Studies on clathrin have mainly focused on secretion from the TGN and endocytosis from the plasma membrane. However, recent studies revealed that clathrin is also an indispensable coat component of vesicles that bud from the endosome (Meyer et al., 2000; Rohn et al., 2000; Hinners and Tooze, 2003). One endosome-to-TGN pathway utilizes AP-1 as a tetrameric adaptor for clathrin. In addition, monomeric adaptor proteins such as EpsinR, TIP47, and PACS-1 are responsible for selecting cargo proteins in endosome-to-TGN transport (Diaz and Pfeffer, 1998; Wan et al., 1998; Saint-Pol et al., 2004). For example, one early endosome-to-TGN pathway that transports TGN38 and Shiga toxin is regulated by EpsinR. In contrast, a late endosome-to-TGN pathway that transports mannose-6-phosphate receptor and furin convertase is regulated by TIP47 and PACS-1.
Vesicle formation not only requires the abovementioned vesicle components (namely, clathrin and its adaptors) but also enzymatic activity. This is supplied by small GTPase ADP-ribosylation factor (Arf; Nie et al., 2003b). Like other small GTPases, Arf cycles between a GTP-bound active state and a GDP-bound inactive state. Its activation and inactivation are regulated by guanine-nucleotide exchange factor (GEF) and GTPase-activating protein (GAP), respectively (Randazzo and Hirsch, 2004; Shin and Nakayama, 2004). The six Arfs are classified into three groups: Arf1, 2, and 3 are class I, Arf4 and 5 are class II, and Arf6 is class III. Among them, Arf1 functions on the Golgi/TGN/endosome. In its active state, Arf1 recruits coat and adaptor proteins such as COPI, AP-1, and AP-3 to the membrane and induces the assembly and nucleation of coated vesicles (Donaldson et al., 1991; Serafini et al., 1991; Traub et al., 1993; Ooi et al., 1998). This localization of the coat/adaptor proteins is sensitive to brefeldin A (BFA) because BFA inactivates Arfs of classes I and II by inhibiting the interaction between Arf and its GEF (Donaldson et al., 1992; Peyroche et al., 1999). Therefore, treatment of cells with BFA causes the disintegration of the Golgi complex and tubulation of the endosomal membrane. In contrast, Arf6 regulates the endocytosis of vesicles from the plasma membrane and the recycling of endosome to the plasma membrane (Donaldson, 2003), and the GEFs for Arf6, ARNO, and EFA are insensitive to BFA (Franco et al., 1999; Peyroche et al., 1999).
The ArfGAP family also contains several members and to date 16 mammalian genes have been reported (Bernards, 2003; Randazzo and Hirsch, 2004). Importantly, it appears that distinct Arf and ArfGAP combinations participate in different intracellular vesicle transport pathways. For example, GAP1 is an Arf1GAP that forms a complex with COPI in an Arf1-dependent manner (Liu et al., 2005) and interacts directly with its cargo protein, a KDEL receptor (Aoe et al., 1999). This interaction is essential for the sorting of cargo proteins into COPI-coated vesicles on the Golgi complex. In contrast, AGAP1, another Arf1GAP, interacts with AP-3 and functions in a late endosome-to-lysosome pathway (Nie et al., 2003a). Moreover, we recently identified SMAP1 as a novel Arf6GAP (Tanabe et al., 2005). SMAP1 interacts with clathrin and regulates the clathrin- and AP-2–dependent endocytosis of transferrin receptors from the plasma membrane.
In this study, we identified a homologue of SMAP1 that we named SMAP2. Like SMAP1, SMAP2 possesses a GAP domain and interacts with clathrin heavy chain (CHC). Furthermore, we show that SMAP2 interacts with the clathrin assembly protein CALM. Unlike SMAP1, however, SMAP2 appears to be a regulator of Arf1 and is involved in clathrin- and AP-1–dependent trafficking in the early endosome-to-TGN pathway. Thus, the SMAP gene family constitutes an important ArfGAP subfamily and each SMAP member exerts common as well as distinct functions in vesicle trafficking.
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MATERIALS AND METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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-adaptin, 100/3) were from Sigma-Aldrich, the mouse mAbs against CHC (X22) and AP-2 (
-adaptin, AP.6), and the rabbit polyclonal antibodies (pAb) against 
-COP and furin convertase were from Affinity BioReagents (Golden, CO), the mouse mAb against AP-3 (
-adaptin) was from Transduction Laboratories (Lexington, KY), the anti-CD25 mouse mAb (7G7/B6) was from Upstate Biotechnology (Lake Placid, NY), the anti-TGN46 sheep pAb was from Serotec (Oxford, United Kingdom), the anti-6His mouse mAb was from Clontech Laboratories (Palo Alto, CA), the Cy3-conjugated goat anti-rat IgG was from Chemicon (Temecula, CA), and the Alexa488-conjugated goat anti-mouse and anti-rabbit IgGs, Alexa488-conjugated donkey anti-sheep IgG and Alexa488-conjugated human-transferrin were from Molecular Probes (Eugene, OR).
Plasmid Construction
A fragment that corresponds to a portion of BC052413
[GenBank]
(GenBank/NCBI) was PCR-amplified from cDNA from a murine erythroleukemic cell line. By using this fragment as a hybridization probe, we screened a cDNA library prepared from the same cells and obtained cDNA clones encoding SMAP2. A cDNA encoding CALM was isolated from the same cDNA library by a PCR method. The various mutations described below were introduced into the coding region of SMAP2 by a PCR method. cDNAs encoding rat GAP1, murine SMAP1, Arf1, and Arf6 were isolated as described previously (Tanabe et al., 2005). TGN38 cDNA was isolated from murine liver-derived cDNA by a PCR method. The human CHC, GGA1, and CD25 cDNAs were provided by Drs. T. Nagase, K. Nakayama, and H. Asao, respectively. The human furin convertase (79823) and EpsinR (KIAA0171) cDNAs were obtained from ATCC and the Kazusa DNA Research Institute, respectively. A chimeric gene encoding an extracellular domain of CD25 fused to the transmembrane and cytoplasmic domains of TGN38 was constructed by a PCR method.
To express these cDNAs in mammalian cells, they were inserted into pcDNA3 (Invitrogen, Carlsbad, CA). For immunodetection, each protein was tagged with the epitope at its amino-terminus as follows: HA-SMAP2, Myc-SMAP2, HA-GAP1, Myc-CALM, Myc-EpsinR, Myc-GGA1, and Myc-furin convertase.
Yeast Two-Hybrid Screening
The Matchmaker Two-Hybrid System 3 (Clontech Laboratories) was used according to the instructions in the manufacturer's manual. The bait plasmid was constructed by inserting the murine SMAP2 cDNA next to the GAL4 DNA-binding domain of the vector pGBKT7. A cDNA library prepared from murine brain was fused to the GAL4 DNA-activation domain of the vector pGADT7 and served as the source of prey plasmids. AH109 cells were used as host cells. The selective medium was devoid of Trp, Leu, His, and Ade and added by 2.5 mM 3-amino-1,2,4-triazole. Plasmid DNAs were recovered from positive clones and sequenced by using an ABI PRISM 310 genetic analyzer (Applied Biosystems, Foster City, CA).
Preparation of Recombinant Proteins
To express cDNAs in bacteria, they were inserted into pGEX5X-3 (Amersham Pharmacia Biotech, Piscataway, NJ). The sequences expressed were the amino-terminal 163 residues of SMAP2, the amino-terminal 255 residues of SMAP1, the amino-terminal 246 residues of GAP1, and the entire coding sequences of Arf1 and Arf6. All were fused at their amino-termini to glutathione S-transferase (GST) and tagged with hexahistidines at their carboxy-termini.
Recombinant proteins were prepared as described previously (Tanabe et al., 2005). Briefly, the Escherichia coli strain BL21 was transformed with one of the expression plasmids and cultured in LB medium containing ampicillin. Isopropyl--D-thiogalactoside was then added at 0.4 mM to induce protein expression. The cells were harvested, and the GST fusion proteins were purified by using a B-PER GST-spin purification kit (Pierce Chemical, Rockford, IL). The fusion protein was digested with Factor Xa (Novagen, Madison, WI) and the GST-free protein was purified by using a 6*His spin purification kit (Pierce Chemical). The protein concentrations were assayed by a BCA protein assay kit (Pierce Chemical). The purity of the recovered proteins was 
95% as judged by SDS-PAGE.
GAP Assay
GAP assays were performed as described in our previous study (Tanabe et al., 2005). Briefly, recombinant nonmyristoylated Arf1 or Arf6 proteins were loaded with [-32P] GTP, and GTP hydrolysis was initiated by the addition of GAP for the indicated time. The reaction was then terminated and unbound nucleotides were removed. GTP and GDP were eluted from the Arf protein and separated by TLC. The plate was exposed to a phosphorimager screen and radioactivity was detected by a Bio Imaging Analyzer (Fuji Photo Film, Tokyo, Japan).
Immunoprecipitation Analysis
Cos-7 cells were transfected with the SMAP2 expression plasmid using Effectene (QIAGEN, Chatsworth, CA) and incubated for 24 h at 37°C. The cells were then lysed with a buffer consisting of 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1% (vol/vol) Nonidet P-40, and a mixture of protease inhibitors (Complete; Roche Diagnostics). The cell lysate was precleared by protein G-Sepharose beads (Amersham Pharmacia) and then incubated with the primary antibody, and the immunoprecipitates were adsorbed to the beads. The beads were washed five times with lysis buffer in which the concentration of NaCl was raised to 200 mM. Proteins were eluted by boiling the beads in SDS sample buffer. Gel electrophoresis of the proteins, transfer of the proteins from the gel to a filter, and immunoreaction with the secondary antibody were performed as described previously (Chiba et al., 1997). Products were visualized by use of the ECL Plus reagent (Amersham Pharmacia).
Immunofluorescence Microscopy
Cos-7 or HeLa cells on coverslips were transfected with plasmid DNA using Effectene and incubated for 24 h at 37°C. They were then fixed with 4% (wt/vol) paraformaldehyde in phosphate-buffered saline (PBS) for 15min at room temperature (RT), washed three times with PBS, and blocked with 1% (wt/vol) bovine serum albumin and 0.1% Triton X-100 in PBS for 15 min at RT. In some cases, the transfected cells were treated with 5 µg/ml BFA for 2 min before fixation. The fixed cells were incubated with appropriately diluted primary and then secondary antibodies for 45 min each at RT. After washing with PBS, the coverslips were mounted on slide glasses using Vectashield (Vector Laboratories, Burlingame, CA) and observed with a confocal microscope (LSM-401, Zeiss, Thornwood, NY). To detect the internalization and transport of transferrin or CD25-TGN38, cells were rinsed with PBS and preincubated in 0.1% (wt/vol) bovine serum albumin in DMEM for 30 min at 37°C. The cells were then incubated at 37°C with 25 µg/ml Alexa488-conjugated human transferrin or 10 µg/ml anti-CD25 mAb for the indicated time, rinsed with PBS, fixed, and processed for immunofluorescence as described above.
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RESULTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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The polypeptide encoded by SMAP2 is composed of 428 aa residues and has a predicted molecular mass of 47 kDa. The alignment of the aa sequences of SMAP2 and SMAP1 is shown in Figure 1A. SMAP2 and SMAP1 share 50% overall sequence homology but four distinct regions in SMAP2, namely, aa 16-125, aa 173-212, aa 244-277, and aa 369-428, show a marked degree of conservation with SMAP1 (85, 74, 69, and 48% homology, respectively; Figure 1B). As described in the following sections, we sought to determine the functions of these conserved regions.
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) and is also conserved in SMAP2 as the 56th residue. When this R56 residue was replaced with glutamine, it reduced or abolished the GAP activity of SMAP2. Therefore, domain aa 1-163 of SMAP2 was determined to have ArfGAP activity (Figure 1B).
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; note that a DLL motif is not conserved in SMAP1). We therefore examined whether SMAP2 also binds CHC (Figure 3A). Thus, Cos-7 cells were transfected or not with SMAP2 tagged with a Myc-epitope, and the cell lysate was immunoprecipitated with anti-CHC antibody (top panel). Probing of the filter with anti-Myc antibody revealed that the transduced SMAP2 was coimmunoprecipitated by the anti-CHC antibody. Conversely, when cells were transfected with SMAP2 tagged with HA, CHC was coimmunoprecipitated by the anti-HA but not the irrelevant anti-Myc antibodies (bottom panel). This indicates the SMAP2 and CHC molecules physically associate inside the cells. In addition, replacement of all the residues in both LLGLD and DLL motifs by alanine abolished the coimmunoprecipitation of CHC (see the lane of SMAP2CBm). Thus, the LLGLD and DLL motifs are essential for SMAP2 to interact with CHC. It must be noted that, unlike the case of SMAP1, mutation of the sole LLGLD motif was not sufficient to disrupt the association of SMAP2 with CHC (unpublished data).
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Collectively, the immunofluorescent results complement those of immunoprecipitation. Thus, based on both, we designate the aa 163-231 region a clathrin-interacting domain (Figure 1B).
Identification of the CALM-interacting Domain in SMAP2
The other two conserved regions, namely, aa 244-277 and aa 369-428, did not show any significant homology to known motifs or domains when we performed BLAST and SMART domain analysis. Therefore, to determine the functions of these regions, we sought to identify molecules that may interact with them by yeast two-hybrid analysis. SMAP2 lacking the amino-terminal aa 1-130 was used as a bait and the prey was a cDNA library prepared from a murine brain. Screening of four million independent clones yielded several positive clones, including a cDNA that encodes a portion of the CALM protein. Consequently, a cDNA clone that harbors the entire CALM protein was reisolated from a library and used in the following studies. This clone was first used to confirm that intact SMAP2 (aa 1-428) and CALM proteins indeed interact in yeast cells (Figure 4A). We then tried to define the CALM-interacting domain in SMAP2 and transduced into yeast cells a portion of SMAP2 that consisted of aa 339-428. This region also interacted with CALM. The observed interaction was specific, because no colony grew on a selective medium if either the bait or prey plasmid harbored no cDNA insert.
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In summary, as shown in Figure 1B, the SMAP2 protein harbors three distinct domains that are responsible for GAP activity, clathrin interaction, and CALM interaction, respectively. The functional significance of the remaining conserved region (aa 244-277) is not known at present.
Subcellular Localization of SMAP2 Protein
In the above experiments of cDNA transfection, we used Cos-7 cells in which transduced SMAP2 was excessively produced and appeared to form aggregates. This was convenient to show whether SMAP2 interacts with CHC or CALM by immunohistochemistry. However, aggregate-like structures are not likely to represent the physiological distribution of SMAP2 inside the cell. The anti-SMAP2 antibody we generated was useful to detect the endogenous protein only by immunoblot but not immunofluorescence (Supplementary Figure S1). Therefore, we transfected HeLa cells with an HA-tagged intact SMAP2; in this case, the SMAP2 protein expression level was moderate (Figure 5). This revealed that SMAP2 was distributed on multiple dotlike as well as juxtanuclear structures. Staining for endogenous CHC displayed a similar pattern to that of SMAP2, and at least some of the CHC fluorescence colocalized with that of SMAP2. However, SMAP2 did not overlap with endogenous COPI, which is a coatmer protein that forms vesicles on the Golgi apparatus.
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). To test whether SMAP2 also overlaps with EpsinR, HeLa cells were cotransfected with HA-SMAP2 and Myc-EpsinR. We found that EpsinR staining colocalized with that of SMAP2. In contrast, HA-SMAP2 did not colocalize with Myc-GGA1, a clathrin-adaptor protein that is utilized in a TGN-to-endosome pathway (Ghosh and Kornfeld, 2004). Thus, SMAP2 is probably involved in AP-1– and EpsinR-mediated vesicle transport. The multiple dot-like fluorescence likely represents the SMAP2 located on the early endosomes, whereas the juxtanuclear fluorescence probably reflects the SMAP2 on endosomes that were located adjacent to the TGN and could not be distinguished from the TGN itself by optical microscopy.
SMAP2 Functions on Arf1 In Vivo
We have shown so far that SMAP2 colocalizes with clathrin, AP-1, and EpsinR on the early endosome/TGN. Judging from its subcellular localization, SMAP2 probably acts as an Arf1GAP in the cell. However, our in vitro GAP assays showed that SMAP2 was equally active with both Arf1 and Arf6 (Figure 2A). Thus, in this section, we examined whether SMAP2 acts on Arf1 in vivo by transfecting HeLa cells with HA-tagged SMAP2R56Q, a GAP-negative mutant. The cells were then processed for double immunofluorescence analysis (Figure 7A). In drug-untreated cells, regardless of the SMAP2R56Q expression, endogenous AP-1 was detected on the endosome/TGN as shown above. The transfected cells were then treated by BFA, an inhibitor of Arf1. In the cells that were not transduced by SMAP2R56Q (indicated by arrowheads), the accumulation of AP-1 on the endosome/TGN was no longer seen, and the AP-1–derived fluorescence was dispersed and abolished. This confirms that the accumulation of AP-1 on the endosome/TGN is an Arf1-dependent process. In contrast, in the SMAP2R56Q-expressing cells (indicated by arrows), the accumulation of AP-1 on membranes persisted and was resistant to BFA treatment. Arf1 in such cells may remain as a GTP-bound form on membranes where SMAP2 is normally the primary ArfGAP. Possible mechanisms how SMAP2R56Q could lead the differently localized AP-1 to become resistant to BFA are discussed later. In any case, the above observations indicate that SMAP2 is likely to function in an Arf1 (and/or the other Arf of classes I and II)-dependent process that involves the endosome.
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Cargo Protein Candidates in the SMAP2-mediated Pathways
We searched which molecules are likely to be transported by the SMAP2-mediated pathways. Judging from the subcellular localization of SMAP2 on the early endosome/TGN, likely candidates are human TGN46 or murine TGN38 (the two are orthologous proteins). Thus, HeLa cells were doubly stained for endogenous TGN46 and the transfected intact HA-SMAP2 protein (Figure 8A). At least some of the TGN46 proteins colocalized with SMAP2 on the TGN. Because endogenous TGN46 was mainly detected on the TGN and not the endosome, we also analyzed cells cotransfected with SMAP2 and CD25-tagged TGN38 (Figure 8B). The fluorescence of TGN38 on the early endosome overlapped with that of SMAP2. These results suggest that TGN38/46 is a likely cargo protein via a SMAP2-dependent process.
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We also found that furin convertase, which is transported from the late endosome to the TGN (Rohn et al., 2000), is not likely to be a cargo protein carried by a SMAP2-dependent pathway, because cotransfected SMAP2 and furin convertase did not colocalize at all (Figure 8D).
Overexpression of SMAP2 Delays the Transport of CD25-TGN38
Finally, we assessed directly whether overexpression of SMAP2 affects the transport of CD25-TGN38 protein from the early endosome to the TGN. Cos-7 cells were cotransfected with HA-SMAP2 and CD25-TGN38 and incubated with anti-CD25 antibody, which triggers the internalization of the chimeric molecule. After the incubation, the cells were fixed and processed for double immunofluorescence (Figure 9). The photos presented in the panels A to F represent the cells after 15 min incubation. The TGN38 protein appeared to reach and accumulate on the TGN in the untransduced cells, whereas the protein remained on the endosomes and did not reach the TGN in the SMAP2-transduced cells (indicated by arrows in A and B). We counted the number of cells that showed or did not show the TGN accumulation of TGN38, respectively. Among the SMAP2-transduced cells, the inhibition of TGN-accumulation was as high as 35% (see the lane of SMAP2 at 15 min in panel G). In contrast, TGN38 reached the TGN in most of mock-transfected cells (see the inhibition of only 4%). Thus, overexpression of intact SMAP2 significantly impairs the transport of CD25-TGN38 from the early endosome to the TGN.
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Possible mechanisms that might explain the differential effects caused by the wild-type as well as mutated SMAP2 proteins are discussed below. At this moment, we conclude that SMAP2 can function in a retrograde transport of CD25-TGN38 from the early endosome to the TGN.
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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SMAP2, SMAP1, and GAP1 all exhibited a lack of preference for both Arf1 and Arf6 in our in vitro GAP assay. This suggests that the ArfGAP domain itself does not necessarily direct the substrate specificity of an ArfGAP toward a particular isoform of Arf. Therefore, to determine which substrate is preferred by SMAP2, we first assessed the subcellular localization of SMAP2. We found that SMAP2 is localized on the AP-1– and EpsinR-positive subcellular structures, but not on the AP-3- or COPI-positive intracellular organelles. Because the combination of AP-1 and EpsinR indicates a specific adaptor for the clathrin-coat on the early endosome (Saint-Pol et al., 2004), the observed colocalization pattern implies that SMAP2 functions in an early endosome-to-TGN pathway that is known to be regulated by Arf1. Indeed, we then found that SMAP2 colocalizes with the TGN38/46 protein and with transferrin molecules on endosomes. Furthermore, we could demonstrate that overexpression of SMAP2 delays the TGN-accumulation of CD25-TGN38, a cargo protein known to be transported from the early endosome to the TGN. This is probably due to the inactivation of Arf1 by SMAP2 and may reflect the impairment of vesicle budding. The observed effects of R56Q and CBm mutants on the transport need some discussion though. A GAP-negative R56Q mutation should keep Arf1 as an active state, thereby likely promoting the vesicle budding. On the other hand, the lack of GAP activity may not allow vesicles to be uncoated. Thus, the apparent accumulation of TGN38 may represent not the TGN itself but actually the endosomes located near the TGN. In the case of CBm mutation, the clathrin molecules may not be recruited to the membranes, thereby resulting in the significant impairment of vesicle budding. In any case, the present study indicates that SMAP2 can function in a retrograde early endosome-to-TGN pathway. This also substantiates the notion proposed by ourselves as well as others that each ArfGAP member acts specifically in a distinct route of vesicle transport inside the cell.
Both SMAP2 and SMAP1 harbor a clathrin box and we showed that both indeed interact with CHC. That both SMAPs interact with clathrin is feasible because the pathways involving SMAP2 (endosome-to-TGN) and SMAP1 (endocytosis from the plasma membrane) are known to utilize clathrin-coated vesicles. The clathrin box in SMAP2 may mediate the indirect interaction between Arf1 and clathrin on the early endosome, similar to the case of SMAP1, as we previously discussed (Tanabe et al., 2005). It must be noted though that, unlike SMAP1, both the classical LLGLD and the atypical DLL motifs appear to contribute to the clathrin-binding in the case of SMAP2. In addition to the SMAPs, Git1 is another example of an ArfGAP that is involved in clathrin-dependent transport. Ligand-dependent endocytosis of G protein–coupled receptors is mediated by Git1 (Claing et al., 2000), and we could detect a canonical clathrin box in Git1 (aa 607-611, unpublished data). A TGN-to-endosome pathway also utilizes clathrin-coated vesicles, but the candidate ArfGAP that may act in this pathway has not been identified so far. Instead, GGA1 is reported to function in this process, and this GGA1 binds not only to clathrin but also to Arf1 (Ghosh and Kornfeld, 2004). Therefore, the presence of clathrin box may be a common feature of the ArfGAPs and GGA1 that are involved in clathrin-dependent vesicle transport.
We could also demonstrate that SMAP2 interacts with CALM and identified a CALM-interacting domain in the carboxy-terminal portion of SMAP2. CALM and AP180 are isoforms and are expressed ubiquitously and specifically in the brain, respectively (Tebar et al., 1999). In cells that are deficient for LAP (an AP180-homologue in Drosophila), the uniformity of clathrin-coated vesicle size is lost (Zhang et al., 1998; Hao et al., 1999). Thus, CALM/AP180 is considered to induce clathrin assembly and to shape the vesicles into an uniform size. Although it is not yet clear whether CALM is involved in vesicle transport from the endosome, it is possible that SMAP2, Arf1, clathrin, and CALM together form a higher order complex at the site of vesicle budding from the endosome.
Treatment of cells with BFA disperses the AP-1 molecules that are accumulated on the early endosomes. We did not see this effect in SMAP2R56Q-transfected cells, although AP-1 and SMAP2R56Q had different localization. One possibility is that the mutant displaces the endogenous SMAP2 from membranes, thereby stabilizing the Arf. An alternative possibility is that the mutant forms a complex with the endogenous SMAP2 and trap it in a nonfunctional state. Observations supporting the latter possibility are described in Supplementary Figure S2. Whichever the mechanisms might be, the observation strongly favors the notion that SMAP2 functions as an Arf1GAP on the early endosomes. In contrast, SMAP1 regulates Arf6-dependent endocytosis from the plasma membrane. Therefore, a reasonable question would be why the homologous SMAP2 and SMAP1 behave so differently in vivo. We found four domains that were conserved between SMAP2 and SMAP1. These correspond to a GAP domain, a clathrin-interacting domain, a domain of unknown function and a CALM-interacting domain. These conserved domains probably do not contain the putative region that is responsible for the different activities of SMAP2 and SMAP1 in vesicle transport. This putative disparate region in the SMAP proteins may be responsible for their different subcellular localizations (SMAP2 on the early endosome and SMAP1 on the plasma membrane) and eventually their specificities for different Arf isoforms (SMAP2 for Arf1 and SMAP1 for Arf6). Thus, these two SMAP proteins, which appear to act in important and distinct pathways of vesicle transport, may help to further dissect the molecular mechanisms involved in these pathways.
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ACKNOWLEDGMENTS |
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Footnotes |
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The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org). 
* These authors contributed equally to this work.
Address correspondence to: Masanobu Satake ( satake{at}idac.tohoku.ac.jp)
Abbreviations used: Arf, ADP-ribosylation factor; BFA, brefeldin A; CHC, clathrin heavy chain; GAP, GTPase-activating protein; GEF, guanine-nucleotide exchange factor; GST, glutathione S-transferase; HA, influenza hemagglutinin; TGN, trans-Golgi network.
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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) or 3 µg (
) of SMAP2 protein, or 6 µg of mutated SMAP2R56Q protein (
) were incubated with 1 µg of GTP-loaded Arf1 (B) or Arf6 (C) for the indicated times. After separating the GDP from GTP on thin-layer chromatograms, the degree of GTP hydrolysis was calculated and is shown as a percentage as in A. Each data point shows the averages and SD values of three independent experiments.
