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Vol. 18, Issue 11, 4681-4689, November 2007

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Adenine Nucleotide (ADP/ATP) Translocase 3 Participates in the Tumor Necrosis Factor–induced Apoptosis of MCF-7 Cells

Ziqiang Yang^*, Wei Cheng, Lixin Hong, Wanze Chen, Yanhai Wang, Shengcai Lin, Jiahuai Han, Huamin Zhou, and Jun Gu^*

*National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China; The Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China; and Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037

Submitted December 29, 2006; Revised August 24, 2007; Accepted September 5, 2007
Monitoring Editor: John Cleveland

	ABSTRACT

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Mitochondrial adenine nucleotide translocase (ANT) is believed to be a component or a regulatory component of the mitochondrial permeability transition pore (mtPTP), which controls mitochondrial permeability transition during apoptosis. However, the role of ANT in apoptosis is still uncertain, because hepatocytes isolated from ANT knockout and wild-type mice are equally sensitive to TNF- and Fas-induced apoptosis. In a screen for genes required for tumor necrosis factor (TNF-)-induced apoptosis in MCF-7 human breast cancer cells using retrovirus insertion–mediated random mutagenesis, we discovered that the ANT3 gene is involved in TNF-–induced cell death in MCF-7 cells. We further found that ANT3 is selectively required for TNF- and oxidative stress–induced cell death in MCF-7 cells, but it is dispensable for cell death induced by several other inducers. This data supplements previous data obtained from ANT knockout studies, indicating that ANT is involved in some apoptotic processes. We found that the resistance to TNF-–induced apoptosis observed in ANT3 mutant (ANT3^mut) cells is associated with a deficiency in the regulation of the mitochondrial membrane potential and cytochrome c release. It is not related to intracellular ATP levels or survival pathways, supporting a previous model in which ANT regulates mtPTP. Our study provides genetic evidence supporting a role of ANT in apoptosis and suggests that the involvement of ANT in cell death is cell type– and stimulus-dependent.

	INTRODUCTION

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MCF-7 human breast cancer cells are widely utilized to study tumor necrosis factor (TNF-)-induced apoptosis. The apoptosis signaling in MCF-7 cells is initiated by TNF receptor I (TNF-RI) clustering that results from TNF and TNF-RI engagement. The clustered TNF-RI recruits the death domain (DD)-containing adapter protein TRADD (TNFRSF1A-associated via death domain) to their intracellular DDs. One function of TRADD is to bind to FADD (Fas-associating protein with death domain), which in turn stimulates caspase-8 autoactivation. Active caspase-8 is an initiator caspase that acts via cytochrome c (cyt c) release from the mitochondria to execute apoptosis in MCF-7 cells (Strasser et al., 2000). TNF-–induced reactive oxygen species (ROS) generation in mitochondria (Goossens et al., 1995) is involved in apoptotic cell death and increases in mitochondrial membrane permeability transition (MPT) are believed to be essential in many apoptosis pathways (Pastorino et al., 1996; Kroemer and Reed, 2000). The loss of inner mitochondrial membrane potential is associated with MPT and has been described as a common step in most apoptotic processes.

MPT is controlled by the mitochondrial permeability transition pore (mtPTP), which is believed to be formed by voltage-dependent anion channel (VDAC), members of the Bcl2 family, cyclophilin D, and adenine nucleotide (ADP/ATP) translocases (ANTs; Zamzami and Kroemer, 2001). The role of cyclophilin D in mtPTP has been confirmed by gene knockouts in mice (Baines et al., 2005; Nakagawa et al., 2005); however, the role of ANT remains controversial (Marzo et al., 1998; Kokoszka et al., 2004). Mitochondria from ANT knockout mouse livers still possess mtPTP activity, contradicting a widely accepted model for mtPTP (Kokoszka et al., 2004). Moreover, sensitivity to TNF- and Fas-induced death is comparable in both ANT-deficient and wild-type hepatocytes (Kokoszka et al., 2004). Nevertheless, the study did reveal using ANT-knockout hepatocytes that ANT has an essential role in modulating the sensitivity of mtPTP to Ca²⁺ activation and ANT ligands (Kokoszka et al., 2004). Therefore, though ANT may not be an essential mtPTP structural component, the interaction of ANT with cyclophilin D and VDAC is still important in regulating mtPTP and perhaps is still required for apoptosis in some cell systems.

In humans there are four ANT isoforms sharing 60–80% identical sequences. ANT1, ANT2, and ANT4 are expressed in a tissue-specific manner, whereas ANT3 is ubiquitously expressed (Stepien et al., 1992; Dolce et al., 2005). Overexpression of ANT1 or ANT3 has been reported to induce apoptosis with a concomitant decrease in mitochondrial inner membrane potential (m), whereas the overexpression of ANT2 had no effect on cell death (Bauer et al., 1999; Zamora et al., 2004). Apart from the potential role in MPT, ANT transports ADP and ATP across the mitochondrial inner membrane, which is essential for mitochondrial ATP synthesis and cytosol ATP supply. Interestingly, a recent study suggests that reducing ATP levels by inhibiting ANT enhances the necrotic process (Temkin et al., 2006). It appears that ANT's role in both MPT and nucleotide translocation are directly or indirectly involved in cell death. However, genetic evidence for the requirement of ANT in apoptosis is still lacking. As for TNF-–induced apoptosis, the role of ANT in cells other than hepatocytes has not been studied.

In search for genes that mediate apoptosis in TNF-–treated MCF-7 cells, we used a random gene disruption approach to generate a series of MCF-7 mutants that are resistant to TNF-–induced cell death (Ono et al., 2001; Wang et al., 2001; Zarubin et al., 2005; da Silva et al., 2006). The gene disrupted in one of the TNF-resistant lines was identified as ANT3. We show that hypomorphic expression of ANT3 in this mutant line is responsible for its low sensitivity to TNF-–induced cell death, providing genetic evidence that ANT3 is required for the TNF-–induced death of MCF-7 breast cancer cells. Our characterization of the ANT3-deficient MCF-7 cells suggests that ANT3 is involved in TNF-–induced MPT and influences ROS production.

	MATERIALS AND METHODS

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Reagents
Recombinant human TNF was purchased from Promega (Madison, WI). zVAD-fmk was purchased from Calbiochem (La Jolla, CA). Cycloheximide (CHX), propidium iodide (PI), valinomycin, oligomycin, H₂O₂, and diquat dibromide (DQ) were purchased from Sigma (St. Louis, MO). DQ, instead of H₂O₂, was used in some of the studies of oxidation stress–induced cell death because it does not need to be freshly made every time. Hydroethidine (HE), DCFH-DA (2',7'-dichlorofluorescin-diacetate), and JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl carbocyanine iodide) were purchased from Molecular Probes (Eugene, OR).

Retroviral Mutagenesis Screening
A clone of an MCF-7 cell, which exhibited a spontaneous survival rate of <1 in 10⁶, was randomly mutated with the retrovirus pDisrup, selected with TNF, and analyzed by the 3' rapid amplification of cDNA ends (RACE) technique as described previously (da Silva et al., 2006). The endogenous gene that was fused with the blasticidin⁺ gene was determined by DNA sequencing of the 3'RACE product. The primers used in the 3'RACE are as follows: primer room temperature (RT) 5'-CCA GTG AGC AGA GTG ACG AGG ACT CGA GCT CAA GC[T]₁₇-3'; nested PCR primers: P1/Q1 (5'-AAA GCG ATA GTG AAG GAC AGT GA-3' and 5'-CCA GTG AGC AGA GTG ACG-3') and P2/Q2 (5'-TGC TGC CCT CTG GTT ATG TGT GG-3' and 5'-GAG GAC TCG AGC TCA AGC-3'). P1 and P2 are located on the balsticidin⁺ gene, whereas Q1 and Q2 are on the anchor sequence of RT.

Constructs
cDNA of ANT3 was cloned into the KpnI and EcoRI sites of the vector pcDNA3 (Invitrogen, Carlsbad, CA), or it was cloned into the XbaI and XhoI sites of the vector pLenti. Annealed oligos for ANT2 and ANT3 RNA interference (RNAi) were cloned into the HpaI and XhoI sites of the vector pLentiLox3.7.

Cell Culture
Wild-type MCF-7 cells, ANT3^mut MCF-7 cells, and 293T cells were maintained in high-glucose DMEM (Invitrogen) supplemented with 10% newborn bovine serum, 100 U/ml penicillin, and 100 µg/ml streptomycin at 37°C and 5% CO₂ in a humidified incubator. In some experiments, cells were changed to glucose-free DMEM (Invitrogen) with 10% dialyzed newborn bovine serum.

Measurement of Cell Survival Rate
Cell survival rates were determined by flow cytometry with two parameters: plasma membrane integrity and cell size. The plasma membrane integrity was tested by the ability of cells to exclude PI. Cells were trypsinized, collected by centrifugation, washed once with phosphate-buffered saline (PBS), and resuspended in PBS containing 1 µg/ml PI. The levels of PI incorporation were quantified on a FACScan flow cytometer (EPICS XL; Beckman Coulter, Fullerton, CA). Cell size was evaluated by forward-angle light scattering. PI-negative cells with a normal size were considered living. The Annexin V-FITC kit (Roche Molecular Biochemicals, Indianapolis, IN) was used to measure PS flipping according to the manufacturer's protocol.

Measurement of ROS
ROS levels were determined by flow cytometry using the probes HE or DCFH-DA (Molecular Probes). HE is ready to be oxidized in the presence of ROS and gives a fluorescence emission that can be detected in the FL2 channel (575-nm filter). DCFH-DA, when inside in the cell, is cleaved by nonspecific esterase and releases carboxydichlorofluorescein. The released group is oxidized by ROS and gives a fluorescence emission that can be detected in the FL1 channel (525-nm filter). Cells were collected and washed once with PBS. The pellets were resuspended in PBS containing 6.6 µM HE or 10 µM DCFH-DA and 1 µg/ml PI, incubated for 45 min at 37°C. PI-negative cells were gated and analyzed by FL2 or FL1 channels, respectively.

Measurement of Mitochondrial m
Fluorescence microscopy or flow cytometry was used to analyze changes in m with the probe JC-1 (Molecular Probes), as described in Ferguson et al. (2003), following the manufacturer's instruction. The cationic dye JC-1 can exist as a monomer or as JC-1 aggregates (J-aggregates), respectively, giving green and red fluorescence emissions. For flow cytometry, cells were collected and then washed once with PBS. The pellets were resuspended in normal medium containing 2.5 µg/ml JC-1. After being incubated for 30 min at 37°C and 5% CO₂, cells were washed twice with PBS and resuspended in PBS. Forward scattering (FSC) versus side scattering (SSC) was used to gate live cells for analysis of green (FL1, 525-nm filter) and red (FL2, 575-nm filter) fluorescence emissions on a FACScan flow cytometer (EPICS XL; Beckman Coulter). The cell population with a large FL2 value and small FL1 value was counted as the percentage of J-aggregate–positive cells. Because the K⁺ ionophore valinomycin disrupts m, valinomycin (5 µg/ml)-treated cells were used as a standard for cells with disrupted m.

Measurement of ATP Levels
ATP was extracted by the boiling Method. Cells (n = 4 x 10⁵) were collected, washed once with PBS, resuspended in 100 µl boiling buffer (100 mM Tris, 4 mM EDTA, pH 7.75), and incubated at 100°C for 2 min. Samples were centrifuged at 10,000 x g for 60 s. ATP levels in the supernatants were determined using the ENLITEN ATP assay kit (Promega). Protein content was determined using the Coomassie brilliant blue G-250 assay kit. The ATP levels were normalized to protein content.

Cell Fractionation and Western Blot Analysis
Cell fractionation was performed mainly as described in Goldstein et al. (2000). Cells (n = 2 x 10⁷) were collected and washed once with ice-cold PBS. The pellets were resuspended in 1 ml ice-cold homogenization buffer (250 mM sucrose, 20 mM HEPES-KOH, pH 7.4, 10 mM KCl, 1.5 mM MgCl₂, 1 mM EDTA, 1 mM EGTA, 1 mM dithiothreitol [DTT], 1 mM phenylmethylsulfonyl fluoride [PMSF], and 10 µg/ml leupeptin). After sitting on ice for 15 min, the cells were disrupted by 200 strokes in a 1-ml Kontes douncer with the B-type pestle (Kontes Glass, Vineland, NJ). The nuclei were removed by centrifugation twice at 1000 x g for 10 min at 4°C. The supernatant was further centrifuged at 17,000 x g for 15 min. The resulting supernatant was the cytosol fraction and was used for Western blot (WB) analysis. For WB analysis, 30 µg of protein was resolved on a 15% SDS-polyacrylamide gel, transferred to a PVDF membrane, and then probed with anti-cyt c or anti-ANT antibodies (1:1000, Santa Cruz Biotechnology, Santa Cruz, CA) for 1 h at RT. After being incubated with horseradish peroxidase–conjugated secondary antibodies (1:2000) for 1 h at RT, the presence of a protein band was visualized using enhanced chemiluminescence (ECL) detection reagents (Amersham Pharmacia Biotech, Piscataway, NJ). The same membrane was stripped (three times using 2% SDS, 50 mM Tris, and 100 mM 2-mercaptoethanol for 20 min at RT and pH 6.5) and reprobed with anti-actin antibodies for 1 h at RT. The membrane was then incubated with secondary antibodies (1 h at RT) and visualized using the ECL system.

Virus Production and Infection
293T cells were used for virus production. Cells were plated in 10-cm dishes 18 h before transfection. The calcium phosphate precipitation method was used to transfect of 80% confluent cells with lentivirus vectors. Cells were changed to fresh medium 12 h later. The medium was collected 40 h later, filtered through a 0.45-µm filter, and centrifuged at 75,000 x g for 2 h at 4°C. The virus pellets were resuspended in fresh medium containing 10 µg/ml polybrene for infection.

Measurement of mRNA Levels
Semiquantitative PCR was performed as described previously (Ono et al., 2001). Forward and reverse primers for ANT1 PCR were as follows: 5'-CTGCCTACTTCGGAGTCTATG-3' and 5'-CTTTCAGTCTGGTTTTATCCC-3'. Primers for ANT2 PCR were as follows: 5'-TCCCAAGAACACTCACATCG-3' and 5'-ATGGAAATGGCTTTAGAGGA-3'. Primers for ANT3 PCR were as follows: 5'-GGGAAAGTCAGGCACAGAGCG-3' and 5'-CGTACAGGACCAGCACGAAGG-3'. For real-time PCR analysis, 0.5 µg of total RNA was used to prepare cDNA using oligo(dT)₁₂ as a primer. The SYBR green PCR Master Mix kit (Applied Biosystems, Foster City, CA) was used for real-time PCR analysis.

RNAi
Vector-based RNAi was used to knock down ANT2 or ANT3 expression. For ANT2 RNAi, the target sequence was GTATCTATGACACTGCAAA; for ANT3, the target sequence was CACATCGTGGTGAGCTGGA.

Electrophoretic Mobility Shift Assay
Nuclear Protein Preparation. Cells (n = 2 x 10⁶) in a 60-mm dish were washed once with PBS, scraped with 1 ml 2.5 mM EDTA-PBS solution and collected by centrifugation at 1500 x g for 5 min at 4°C. The cell pellets were resuspended in 160 µl of cold buffer A (10 mM HEPES, pH 7.9, 1.5 mM MgCl₂, 10 mM KCl, 1 mM DTT, 1 mM PMSF, and 10 µg/ml aprotinin) by gentle pipetting. The cells were allowed to swell on ice for 15 min, and then 40 µl of 2.5% NP-40 in buffer A was added and vigorously vortexed for 10 s. The nuclear pellets were collected by centrifugation at 1500 x g for 5 min at 4°C, resuspended in 40 µl of ice-cold buffer C (20 mM HEPES, pH 7.9, 0.4 M NaCl, 1 mM EDTA, 1 mM DTT, and 1 mM PMSF), vortexed for 25 min at 4°C, and then centrifuged at 15,000x g for 5 min at 4°C. The supernatant was frozen in aliquots at –70°C.

Gel Shift Assay. The probes were chemically synthesized, 3' end biotin-labeled using the Biotin 3' End DNA Labeling Kit (Pierce, Rockford, IL), and annealed following the manufacturer's instructions. Sense and antisense probe sequences for the nuclear factor-B (NF-B)-binding site were as follows: 5'-AGT TGA GGG GAC TTT CCC AGG C-3' and 3'-TCA ACT CCC CTG AAA GGG TCC G-5'. EMSA was performed following the LightShift Chemiluminescent EMSA Kit instruction (Pierce). Briefly, 8 µg nuclear protein, 1 µg poly(dI:dC) were incubated in the reaction buffer containing 10 mM Tris (pH 7.5), 50 mM KCl, 5 mM MgCl₂, 1 mM DTT, 2.5% glycerol, and 0.05% NP40 for 10 min at RT, and then 50 fmol biotin-labeled probes were added and incubated for another 20 min at RT. After incubation, 2 µl loading buffer containing 0.3% bromophenol blue/3% glycerol was added. The mixtures were separated by electrophoresis through a nondenaturing 4% polyacrylamide gel. Then the binding complexes were transferred to a nylon membrane. Transferred DNA was cross-linked to membrane by UV. The biotin labeled DNA was detected by chemiluminescence.

Southern Blot Analysis
Genomic DNA from wild-type and ANT3^mut MCF-7 cells was digested with EcoRI and XmnI. The DNA fragments were resolved by 1% agarose gel electrophoresis, blotted onto a nitrocellulose membrane, and detected with a random-primed ³²P-labeled probe obtained using a DNA fragment containing exon 4 of human ANT3.

Statistical Analysis
Student's t test was used.

	RESULTS

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ANT3 Is Involved in the TNF-–induced Apoptosis of MCF-7 Cells
Because the MCF-7 breast cancer epithelial cell line is frequently used as a model to study TNF-–induced apoptosis, we used it to identify genes required for TNF-–induced cell death by using a retroviral-induced mutagenesis approach (da Silva et al., 2006). Retroviral integration can generate mutant alleles resulting in diminished or abolished expression of the target gene. Cells with mutations in genes required for TNF-–induced killing may become TNF resistant and may be selected by TNF treatment. We selected a number of TNF resistance clones and determined that one of the resistant clones contained a disrupted ANT3 gene; we termed this clone ANT3^mut.

Figure 1A shows that ANT3^mut cells are resistant to TNF-–induced cell death unlike parental wild-type MCF-7 cells. The ANT3^mut cells are also resistant to TNF+CHX-induced cell death, though to a lesser extent (Figure 1B). The retroviral insertion in ANT3^mut cells was located 219 nucleotides downstream of the stop codon. Figure 1C shows a Southern blot analysis of the ANT3 gene disruption in ANT3^mut cells. A probe containing the exon 4 sequence of ANT3 detected a bigger fragment in EcoRI+XmnI-digested genomic DNA, indicating that one allele of ANT3 contains a DNA insertion. We described in our previous report that our specially designed retroviral vector contains a self-cleavage ribozyme sequence that would diminish gene expression by cleavage the fused mRNA if the insertion occurred within the 3' untranslated region (Zarubin et al., 2005). Semiquantitative PCR (Figure 1D) and real-time PCR (data not shown) confirmed the reduction of ANT3 mRNA in ANT3^mut cells, suggesting that a functional ANT3 allele was indeed disrupted. We analyzed the other two ANT family members in wild-type and ANT3^mut cells and found that ANT1 mRNA is undetectable in MCF-7 cells, and ANT2 mRNA is slightly higher in ANT3^mut cells (Figure 1D). We have obtained an anti-ANT antibody, which detects all forms of ANT. WB analysis of the lysates from wild-type and ANT3^mut cells with anti-ANT antibody did not reveal a difference between these two cells (data not shown). Because ANT2 and ANT3 are not distinguishable in molecular weight, we believe this result reflects no significant difference of the total ANT proteins between wild-type and ANT3^mut cells.

View larger version (35K): [in this window] [in a new window]   Figure 1. ANT3 mutation in MCF-7 cells led to a resistance to TNF-–induced cell death. (A) Parental wild-type (WT) and ANT3mut MCF-7 cells were treated with TNF- (50 ng/ml) for different periods of time, and cell survival rates were measured by PI exclusion. (B) WT and ANT3mut cells were treated with TNF- (50 ng/ml) + CHX (1 µg/ml) for different periods of time, and cell survival rates were measured by PI exclusion. (C) Southern blot analysis of genomic DNA digested with EcoRI and XmnI. A probe containing the exon 4 sequence of ANT3 was used. (D) Semiquantitative RT-PCR was performed using the total RNA from WT and ANT3mut cells to determine the mRNA levels of ANT1, ANT2, and ANT3. GAPDH was used as control. An ethidium bromide stain is shown. (E) siRNA of ANT2 or ANT3 was used to treat WT and ANT3mut cells for 3 d, and the ANT3 or ANT2 protein levels were analyzed by WB using anti-ANT antibody. (F) ANT3mut cells were infected with a lentivirus encoding GFP or ANT3, and the resulting cells were designated as ANT3mut+GFP or ANT3mut+ANT3, respectively. Thirty-six hours after infection, these cells were treated with TNF- (50 ng/ml) for different periods of time, and cell survival rates were measured by PI exclusion. ANT3 mRNA levels in these cells were determined by semiquantitative RT-PCR. The ectopic expression of ANT3 was confirmed by Western blotting with anti-ANT antibody. (G) WT cells were infected with lentiviruses encoding siRNA targeting ANT3 or a control lentivirus. The resulting cells were designated as siANT3 or siVector, respectively. Forty-eight hours after infection, these cells were treated with TNF- (50 ng/ml) for different periods of time, and the cell survival rates were measured. ANT3 mRNA levels in these cells were determined by semiquantitative RT-PCR. (H) The same as E except siANT2 was used. Comparable results were obtained in 2–5 independent experiments. The representative data are shown.

The ANT3^mut Line Is Resistant to Oxidative Stress–induced Cell Death
To establish whether ANT3^mut is selectively resistant to different death triggers, we examined its sensitivity to several death stimuli. In comparison with wild-type MCF-7 cells, ANT3^mut cells are 1) resistant to cell death induced by H₂O₂ and by the superoxide anion producer DQ (Farrington et al., 1973; Figure 2, A and B). 2) They are moderately resistant to cell death induced by glucose starvation and by the microtubule disruptor vincristine (Figure 2, C and D). 3) They are almost equally sensitive to cell death induced by the alkylating agent mitomycin, the pyrimidine analogue 5-fluorouracil, the mitochondrial ATPase inhibitor oligomycin, and the K⁺ ionophore valinomycin (Figure 2, E, F, G, and H). Restoring ANT3 expression in ANT3^mut cells increased TNF-, H₂O₂-, and glucose starvation–induced cell death (Figure 2I), which confirmed the role of ANT3. Because of the role of ANT in transporting ADP from the cytosol into mitochondria for ATP synthesis, it is worthwhile to note that oligomycin, which inhibits electron transport in mitochondria, and valinomycin, which induces a loss in the inner mitochondrial membrane potential, both induce similar levels of cell death in ANT3^mut and wild-type MCF-7 cells. Because ROS is involved in TNF-–induced apoptosis, the data in Figure 2 suggest that ANT3 participates in the death process mediated and/or triggered by free radicals.

View larger version (32K): [in this window] [in a new window]   Figure 2. ANT3mut MCF-7 cells are resistant to oxidative stress–induced cell death. WT and ANT3mut cells were either treated with different concentrations of H2O2 (A), DQ (B), vincristine (D), mitomycin (E), 5-fluorouracil (F), or valinomycin (H) for 18, 24, 96, 96, 96, and 72 h, respectively, or they were incubated with glucose-free medium (C) or 20 µM oligomycin (G) for different periods of time. Cell survival rates were measured by PI exclusion. (I) ANT3mut cells were infected with a lentivirus encoding GFP or ANT3. Thirty-six hours after infection, these cells were treated with TNF- (50 ng/ml), H2O2 (1 mM), or glucose starvation for 24 h, and cell survival rates were measured by PI exclusion. *p < 0.005, ** p < 0.05 (Student's t test). Data are the mean ± SD of triplicate samples.

View larger version (29K): [in this window] [in a new window]   Figure 3. ANT3 mutation–mediated death resistance is not related to cellular ATP levels or the NF-B pathway. (A) WT and ANT3mut cells were left untreated (Ctrl) or were treated with TNF- (50 ng/ml), DQ (0.5 mM), glucose starvation, or oligomycin (10 µM) for 12, 12, 9, and 9 h, respectively. Cells were collected and relative ATP levels were determined using the Enliten ATP assay kit (Promega). (B) WT and ANT3mut cells were treated with TNF- (50 ng/ml) for different periods of time. NF-B activity was measured by EMSA. Comparable results were obtained in three independent experiments. The representative data are shown.

Cyt c Release in Apoptosis Is Impaired in ANT3^mut MCF-7 Cells
We next sought to determine the location of ANT3 in the TNF-–induced apoptosis pathway. It is well established that TNF-–induced apoptosis is initiated by caspase-8 activation. As expected, the inhibition of caspases by the pan caspase inhibitor zVAD blocked TNF-–induced death in both wild-type and ANT3^mut MCF-7 cells (Figure 4A). Although TNF-–induced cell death was significantly different between wild-type and ANT3^mut cells, zVAD treatment eliminated this difference by preventing apoptosis in both cells. This is consistent with the fact that ANT3 is a mitochondrial protein and that the mitochondria functions downstream of the initiator caspase in the apoptosis pathway. For a comparison, we also treated wild-type and ANT3^mut cells with DQ alone or together with zVAD and examined cell viability (Figure 4B). zVAD only slightly inhibited cell death in both wild-type and ANT3^mut cells, and the levels of cell death were still different between wild-type and ANT3^mut cells. Because MCF-7 cells lack caspase-3, a major execution caspase, the cell death pathway downstream of the mitochondria in MCF-7 cells is not very dependent on caspase (Janicke et al., 1998). The modest effect of zVAD on DQ-induced cell death suggests that DQ-induced cell death is primarily effector caspases-independent. To confirm that ANT3 regulates mitochondrial events in apoptosis, we measured cyt c release from the mitochondria in TNF--treated wild-type and ANT3^mut cells. As with previous reports, the release of cyt c into the cytosol was detected in TNF-–treated wild-type MCF-7 cells (Figure 4C). We found that the TNF-–induced release of cyt c was totally impaired in ANT3^mut cells (Figure 4C). Similarly, DQ-induced cyt c release was also totally impaired in ANT3^mut cells (Figure 4D). Collectively, these observations indicate that the function of ANT3 in TNF-–induced but not DQ-induced apoptosis is controlled by signaling from an initiator caspase. Additionally, ANT3 is directly or indirectly involved in cyt c release in both TNF- and DQ-induced cell death in MCF-7 cells.

View larger version (30K): [in this window] [in a new window]   Figure 4. ANT3mut cells have impaired cyt c release in apoptosis. (A) WT and ANT3mut cells were treated with TNF- alone (50 ng/ml) or TNF- (50 ng/ml) + zVAD (1 µM) for different periods of time. Cell survival rates were measured by PI exclusion. (B) WT and ANTmut cells were treated with DQ (0.5 mM) alone or DQ (0.5 mM) + zVAD (1 µM) for different periods of time. Cell survival rates were measured by PI exclusion. (C) WT and ANT3mut cells were treated with TNF- (50 ng/ml) for different periods of time. Cells were collected and subjected to fractionation. cyt c levels in the cytosol fraction were analyzed by Western blotting. -actin levels were measured as a loading control. (D) WT and ANT3mut cells were treated with DQ (0.5 mM) for different periods of time. Cells were subjected to analysis of cyt c release as in C. Comparable results were obtained in 2–3 independent experiments. The representative data are shown.

TNF-–induced Mitochondrial _m Depolarization Is Attenuated in ANT3^mut Cells

View larger version (28K): [in this window] [in a new window]   Figure 5. Comparison of m changes and CsA's effect in WT and ANT3mut cells. WT and ANT3mut cells were treated with TNF- (50 ng/ml; A) or DQ (0.5 mM; B) or were subjected to glucose starvation (C) for different periods of time. Cells were collected, and the mitochondria inner membrane potential (m) in live cells (PI-negative cells) was analyzed. J-aggregate–positive cells are those with normal m values. Cell death was measured by PI exclusion (D) or annexin staining (E) in WT and ANT3mut cells that were treated with or without TNF- for 24 h in the presence or absence of 5 µM CsA. *p < 0.01 (Student's t test). Comparable results were obtained in two independent experiments. The representative data are shown.

Valinomycin is a K⁺ ionophore that can disrupt _m. At a concentration of 5 µg/ml, valinomycin does not cause significant cell death for up to 36 h in both wild-type and ANT3^mut MCF-7 cells (Figure 6A), but it is sufficient to induce _m depolarization (Figure 6B). As with its effect on cell death when higher doses or longer incubations were used (Figure 2H), valinomycin-induced _m depolarization is the same in wild-type and ANT3^mut cells (Figure 6B). In contrast, TNF-–induced _m depolarization was much less in ANT3^mut cells (Figure 5A). When a treatment of TNF together with 5 µg/ml valinomycin was used, significant cell death occurred and no difference was found between wild-type and ANT3^mut cells (Figure 6C). Similarly, _m was lost in TNF+valinomycin–treated cells, and no differences were observed between wild-type and ANT3^mut cells. Valinomycin appears to be able to eliminate the difference between TNF-–treated ANT3^mut and wild-type MCF-7 cells. These data support the idea that ANT3 mutation–mediated cell death resistance is related to its selective interference of TNF-–induced _m depolarization.

View larger version (29K): [in this window] [in a new window]   Figure 6. Disrupting m by valinomycin overcomes the difference between WT and ANT3mut cells with respect to TNF-–induced cell death. (A) WT and ANT3mut cells were treated with valinomycin (5 µg/ml) for different periods of time. Cell survival rates were measured by PI exclusion. (B) m in cells described in A were analyzed as described in Figure 5. (C) WT and ANT3mut cells were treated with TNF- (50 ng/ml) together with valinomycin (5 µg/ml) for different periods of time. Cell survival rates were measured. (D) Percentages of J-aggregate–positive cells in cells described in C. Comparable results were obtained in two independent experiments. The representative data are shown.

ANT3 Mutation Alters TNF-–induced ROS Response in MCF-7 Cells

View larger version (31K): [in this window] [in a new window]   Figure 7. ANT3 mutation in MCF-7 cells alters TNF- and glucose starvation–induced ROS production. (A) WT and ANT3mut cells were treated with TNF- (50 ng/ml) for different periods of time. Cells were collected, and live cells were gated for ROS analysis using HE or DCFH-DA. (B) WT and ANT3mut cells were changed to glucose-free medium for different periods of time. ROS levels were analyzed as in A. (C) siANT3- or control vector-treated MCF-7 cells were stimulated with TNF- for time periods as indicated. ROS levels in live cells were analyzed using DCFH-DA. (D) The same as C except ANT3 was ectopically expressed in MCF-7 cells before the analysis. Comparable results were obtained in 2–3 independent experiments. The representative data are shown.

	DISCUSSION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 
Although ANTs are dispensable for TNF-–induced death in hepatocytes (Kokoszka et al., 2004), our genetic screen revealed that ANT3 is involved in TNF-–induced MCF-7 cell death, suggesting that ANT participates in cell death in some systems. Cells with hypomorphic ANT3 expression are selectively resistant to some death stimuli, but not the others, which is consistent with the idea that ANT3 is dispensable for some types of cell death. A recent report that knockdown of ANT3 induces T-cell apoptosis (Jang and Lee, 2006) supports the involvement of ANT3 in cell death and further suggests that ANT3 can both positively and negatively influence cell death depending on cell systems. As with previously proposed models, ANT3 is indeed involved in the regulation of mtPTP in MCF-7 cells. This is evident by the observation that ANT3 mutation impaired the _m depolarization and subsequent release of cyt c from the mitochondria that normally follows exposure to TNF and other stimuli. Our study provides genetic evidence in support of the role of ANT3 in regulating mtPTP and cell death in some systems.

Although the role of mtPTP in cell death has been well established, gene knockout of cyclophilin D and ANT (Kokoszka et al., 2004; Nakagawa et al., 2005; Baines et al., 2005), as well as our work describe herein, suggest there is significant complexity in this death control pore. Cyclophilin D is required for some necrotic, but not apoptotic, forms of cell death that were tested. ANT is not required for TNF- and Fas-induced hepatocyte death, but it is involved in TNF- and oxidative stress–induced MCF-7 cell death. Perhaps cyclophilin D and ANTs are regulatory components of mtPTP, and each of them senses different signals. If this is true, then the complexity also exists in the upstream signal pathway, because ANT's role in cell death is clearly cell type– and stimulus-dependent.

A number of studies suggest that oxidative stress is involved in TNF-–induced cell death. Previous studies have shown that enhancing ROS production in TNF-–treated MCF-7 cells by overexpressing NAD(P)H:quinone reductase or by treating the cells with vitamin D increases TNF-–induced cell death (Siemankowski et al., 2000; Weitsman et al., 2003). However, TNF-–induced ROS production in MCF-7 cells has not been extensively studied. We show that TNF induces biphase ROS production in MCF-7 cells. This biphase ROS production may relate to cell death because ANT3 mutation impairs the biphase ROS response to TNF stimulation. It needs to be noted that although many studies suggest that sustained ROS production causes damage, ANT3^mut cells, which have a sustained ROS increase upon TNF treatment or glucose starvation, have less cell death. Although we do not know the exact link between cell death and the pattern and levels of ROS in our system, our data suggests that TNF-–induced apoptosis in MCF-7 cells is independent of the effect of ROS levels.

In vitro studies have shown that Ca²⁺ increases the generation of H₂O₂ by some unknown mechanism that is inhibited by CsA (Kanno et al., 2004), a drug that binds to cyclophilin D, which in turn prevents cyclophilin D from binding to ANT and strongly inhibits mtPTP, suggesting that ANT is involved in ROS production. In the presence of inorganic phosphate, Ca²⁺ increases oxygen consumption and ROS production in isolated mitochondria, while decreasing the content of free thiols in ANT (Kanno et al., 2004). We do not know if the role of ANT3 in the biphase ROS production is due to it counteracting ROS production or if it is targeted by ROS and thus decreases ROS in MCF-7 cells. It is hard to believe that more ROS in ANT3^mut cells leads to less cell death because it cannot be explained by current knowledge. Because of the possibility that ANT3 is a target of ROS, the ROS decrease observed in wild-type MCF-7 cells after 4 h of TNF treatment (Figure 7A) could be a result of the neutralization of ROS by ANT3. Therefore, it can be speculated that although ROS levels in wild-type cells were low at 4 h after TNF treatment, ANT3 had been damaged and MPT might have been induced. If this is true, we can also speculate that the loss of ANT3 might make the mtPTP insensitive to ROS so that even if TNF induced a sustained ROS elevation the cell death was still less.

Although cell death is associated with _m depolarization, the frequency of cell death in TNF-, DQ-, and valinomycin-treated cells does not precisely correlate with the level of _m depolarization. For example, TNF and DQ treatment resulted in similar cell death frequencies in MCF-7 cells (Figures 1A and 2B), but the percentage of cells positive for J-aggregates differs (Figure 5, A and B). In addition, 5 µg/ml valinomycin can effectively trigger _m depolarization, but its induced cell death is modest (Figure 6, A and B). Similarly, ROS is associated with cell death, but the level of ROS induction does not correlate well with cell death (Figure 7, A and B). The differences between ROS production in glucose-starved wild-type and ANT3^mut cells are bigger than that between TNF-–treated wild-type and ANT3^mut cells, but ANT3^mut cells are less protected against glucose starvation than against TNF treatment. Therefore, an apoptotic process could be influenced by multiple factors in the mitochondria. It is clear that much more work is needed to understand how mtPTP is regulated. Our study presented here demonstrates that ANT3 is required for some apoptotic cell death. Our data supports the idea that the role of ANT3 in cell death is to regulate MPT.

	ACKNOWLEDGMENTS

 
This work was supported in part by Grants NSFC30670442 and NSF30330260 and Grant NSFC-CIHR 30611120526 from Chinese National Science Foundation; Grants IRT0649 and J0630649 from Ministry of Education of China; and funds from Xiamen University.

	Footnotes

 
This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-12-1161) on September 12, 2007.

Address correspondence to: Huamin Zhou (huaminzhou{at}xmu.edu.cn) or Jun Gu (gj{at}pku.edu.cn)

Abbreviations used: ANT, adenine nucleotide translocase; MPT, mitochondrial permeability transition; TNF, tumor necrosis factor.

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